Case report Yeo-Jin Song1*, Bongyoung Kim1*, Young-eun Kim2, Yangsoon Lee2, Hyunjoo Pai1 1Department of Internal Medicine, 2Department of Laboratory Medicine, Hanyang University College of Medicine, Seoul, Korea *These authors contributed equally to this work. Corresponding to Hyunjoo Pai, E-mail: paihj@hanyang.ac.kr Ann Clin Microbiol 2020;23(4):211-216. https://doi.org/10.5145/ACM.2020.23.4.6Received on 19 February 2020, Revised on 10 April 2020, Accepted on 10 April 2020, Published on 20 December 2020.Copyright © Korean Society of Clinical Microbiology. Abstract Rothia spp. are aerobic, gram-positive cocci belonging to the family Micrococcaceae, and are a part of the normal microbial flora of the human oropharynx and upper respiratory tract. We present the first case of the prosthetic valve endocarditis with cerebral hemorrhage caused by Rothia mucilaginosa in South Korea. A 65-year-old man with a prosthetic aortic valve visited the outpatient clinic with a complaint of fever. R. mucilaginosa was identified in one among four sets of blood culture bottles obtained

Book review Young Ah Kim1,2, Hyunsoo Kim1,3, Chulhun L. Chang1,4 1Korean Society of Clinical Microbiology, 2Department of Laboratory Medicine, National Health Insurance Service Ilsan Hospital, Goyang, 3Department of Laboratory Medicine, National Police Hospital, Seoul, 4Department of Laboratory Medicine, Pusan National University Yangsan Hospital, Yangsan, Korea Corresponding to Chulhun L. Chang, E-mail: cchl@pusan.ac.kr Ann Clin Microbiol 2020;23(3):159-163. https://doi.org/10.5145/ACM.2020.23.3.6Received on 27 April 2020, Revised on 19 August 2020, Accepted on 21 August 2020, Published on 20 September 2020.Copyright © Korean Society of Clinical Microbiology. Abstract The Coronavirus Disease 19 (COVID-19), which emerged as pneumonia from an unknown agent for the first time at the end of 2019, has dramatically transformed our world into one that is highly unrecognizable today. Newly emerging infectious diseases have been occurring more frequently than ever. Opportunities of such deadly microorganisms to adapt to humans—as well as spread between people on a massive scale—are growing because of active

Original article Hyunmi Cho1, Jong-Bae Kim2, Young Uh1 1Department of Laboratory Medicine, Wonju Severance Christian Hospital, Yonsei University Wonju College of Medicine, Wonju, 2Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Korea Corresponding to Young Uh, E-mail: u931018@yonsei.ac.kr Ann Clin Microbiol 2020;23(1):21-31. https://doi.org/10.5145/ACM.2020.23.1.21Received on 12 June 2019, Revised on 19 August 2019, Accepted on 19 August 2019, Published on 20 March 2019.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Rapid and accurate detection of Mycobacterium tuberculosis (MTB) is of primary importance for infection control and selection of anti-tuberculosis drugs. However, most clinical laboratories report MTB complex (MTC) without reporting MTB because MTC comprising MTB, Mycobacterium bovis, Mycobacterium africanum, Mycobacterium microti, Mycobacterium capraei and Mycobacterium pinnipedii have 99.9% similarity at the nucleotide level and identical 16S rRNA sequences. This study was conducted to analyze the species frequency of MTC isolates obtained from clinical specimen. Methods: Of

Case report PDF Hyun Lim, M.D., Dal Sik Kim, M.D., Hye Soo Lee, M.D. and Sam Im Choi, M.D. Department of Clinical Pathology, Chonbuk National University Medical School, Chonju, Korea Corresponding to Hye Soo Lee Ann Clin Microbiol 1999;2(2):207-211.Copyright © Korean Society of Clinical Microbiology. Abstract Thelazia callipaeda is a slender, long, and cylindrical nematode which parasitizes in the conjunctival sac of human and causes conjunctivitis. The animals such as the dog, rabbit, horse, deer, and cow were revealed as its reservoir and some species of the fly suspected as its vector. We experienced a case of T. callipaeda isolated from human conjunctival sac of a 41-year old man who lived in Wanju-gun, Chonbuk province and raised the dogs. He complained of an irritation, itching and foreign body sensation on his right eye and the two worms were picked out of his right eye by forceps from conjunctival sac. General features of the

Original article PDF Sook Won Ryu, M.D., Chang Kyu Lee, M.D., Chang Su Kim M.D., Do Hyun Lee, M.T., Young Kee Kim, M.D. and Kap No Lee, M.D. Department of Clinical Pathology, College of Medicine, Korea University, Seoul, Korea Corresponding to Chang Kyu Lee Ann Clin Microbiol 1999;2(2):199-206.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The incidence of reported nontyphoidal Salmonellosis has increased during last decade in Korea. Salmonella typhimurium and Salmonella enteritidis are two major serotypes in nontyphoidal Salmonella. To determine the nature of potential outbreak S. typhimurium infection in a community, we retrospectively evaluated clinical and epidemiologic features of S. typhimurium infections and performed pulsed-field gel electrophoresis (PFGE) to investigate a genetic relatedness of S. typhimurium isolated in Guro Hospital. Methods: From May 1998 to April 1999, a total of 20 S. typhimurium strains were isolated from 18 patients. PFGE patterns were analyzed for 20 S. typhimurium strains. Clinical and epidemiological features were evaluated from their medical records. Results:

Original article PDF Yoon Hee Kang, M.D., Soo Jin Choi, M.D., Sang Hyun Hwang, M.D., Young Wook Cho, M.D., Duck-Hee Kim, M.T.*, Mi-Na Kim, M.D. and Chik Hyun Pai, M.D. Department of Clinical Pathology, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Korea Corresponding to Mi-Na Kim Ann Clin Microbiol 1999;2(2):158-166.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Escherichia coli and Klebsiella pneumoniae resistant to 3rd generation cephalosporin have been reported with increasing frequency in tertiary-care hospital in Korea. MicroScan Neg Combo Panel Type 21 (Type 21) contains a 1 µg/mL cepfoxodime (POD) in addition to other screen wells containing ceftazidime, cefotaxime, ceftriaxone, and aztreonam, which are designed for detecting extended-spectrum β-lactamase (ESBL)-producing E. coli and Klebsiella species. We evaluated the Type 21 panel for its ability to detect ESBL. Methods: From November to December in 1998, 496 E. coli and 326 K. pneumoniae strains isolated from clinical specimens were tested with Type 21 panel. The isolates

Original article PDF Jongwook Lee, M.D., Soo Hwan Pai, M.D., Jong Hyun Nahm, M.D., Jong Won Choi M.D., Bum Su Kim*, M.D., Won Choi*, M.D., Don Haeng Lee*, M.D., Hyung Gil Kim*, M.D. and Young Soo Kim*, M.D. Departments of Clinical Pathology, Internal medicine*, Inha University Medical College, Inchon, Korea Corresponding to Jongwook Lee Ann Clin Microbiol 1999;2(1):54-57.Copyright © Korean Society of Clinical Microbiology. Abstract Background：The rapid urease test has been widely used for detection of Helicobacter pylori infection because it is easy, simple and rapid result. The CLO test and PyloriTek test observe color changes after the gastric biopsy specimens are inerted into the test kits. Because H. pylori is not evenly distributed in the gastric mucosa, grinding or mincing of gastric specimens prior to culture enhances isolation rate of H. pylori. Therefore, we evaluated the effects of rubbing the gastric biopsy specimens onto the urea soaked filter paper in this home-made rapid urease

Original article PDF Young Uh*, Soon Deok Park, Jeong Seog Son*, Hyeun Gyeo Lee*, An Suk Jeoung*, Hyun Mi Cho*, and Kap Jun Yoon*, and Hyo Youl Kim Departments of Clinical Pathology* and Internal Medicine**, Yonsei University Wonju College of Medicine, Wonju, and Research Institute of Bacterial Resistance***, Yonsei University College of Medicine, Seoul, Korea Corresponding to Young Uh Ann Clin Microbiol 2000;3(2):99-110.Copyright © Korean Society of Clinical Microbiology. Abstract Background: In recent years, the incidence of extended-spectrum β-lactamase (ESBL) producing Klebsiella has been steadily increased, and the newer species K. planticola and K. terrigena, formerly regarded as nonpathogen, have been reported with astonishing frequency from human infectious processes by some investigators. The aim of this study is to elucidate the isolation rate and antimicrobial susceptibility of recent clinical Klebsiella isolates. Method: For the clinical Klebsiella isolates during the period of June 1999 to May 2000, isolation frequency of Klebsiella species by specimen, departments, age, and sex were analyzed. And antimicrobial susceptibilities

Original article PDF Eui Chong Kim, M.D.¹,², Dong Young Lee, M.D.¹, Hae Shim Choi, M.S.¹, Se Ik Joo, M.S.¹, Jung Hee Lee, M.S.³, Sang Hyun Kim, M.D.² and Sung Hwan Ban, M.D.³ ¹Department of Clinical Pathology, Seoul National University College of Medicine; ²Clinical Research Institute, Seoul National University Hospital, Seoul; ³Department of Pediatrics, Kumi Hospital, Soonchunhyang University College of Medicine, Kumi, Korea Corresponding to Eui Chong Kim, E-mail: euichong@plaza.snu.ac.kr Ann Clin Microbiol 2000;3(2):94-98.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Shiga toxin-producing Escherichia coli (STEC) was found in several serotypes of E. coli including O157 serotype. Sorbitol-MacConkey agar may be useful for the detection of E. coli O157, but is not helpful for the detection of sorbitol-fermenting STEC other than O157. Moreover, some strains of E. coli O157 can ferment sorbitol. In this study, in situ hybridization using DNA probe of shiga toxin was used for the isolation of STEC from the PCR-positive stool and sequence analysis