Original article PDF Hee-Jung Kim1, Nam Yong Lee1, Sunjoo Kim2,3, Jeong Hwan Shin4,5, Mi-Na Kim6, Eui-Chong Kim7, Sun Hoi Koo8, Nam Hee Ryoo9, Jae-Seok Kim10, Ji-Hyun Cho11 1Department of Laboratory Medicine & Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, 2Department of Laboratory Medicine, 3Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju, 4Department of Laboratory Medicine, 5Paik Institute for Clinical Research, Inje University College of Medicine, Busan, Department of Laboratory Medicine, 6Asan Medical Center and University of Ulsan College of Medicine, 7Seoul National University Hospital, Seoul, 8College of Medicine, Chungnam National University, Daejeon, 9School of Medicine, Keimyung University, Daegu, 10Hallym University College of Medicine, Seoul, 11College of Medicine, Wonkwang University, Iksan, Korea Corresponding to Nam Yong Lee, E-mail: micro.lee@samsung.com Ann Clin Microbiol 2011;14(2):48-54. https://doi.org/10.5145/KJCM.2011.14.2.48Copyright © Korean Society of Clinical Microbiology. Abstract Background: Blood culture is important for determining the etiologic agents of bacteremia and fungemia.

Case report PDF Eui Chong Kim1, Jeong Hwan Shin2, Sunjoo Kim3, Nam Yong Lee4, Ji-Hyun Cho5, Sun Hoe Koo6, Nam Hee Ryoo7, Sae Ick Joo1 Departments of Laboratory Medicine, 1Seoul National University College of Medicine, Seoul, 2Inje University College of Medicine, Busan, 3Gyeongsang National University School of Medicine, Jinju, 4Sungkyunkwan University School of Medicine, Samsung Medical Center, Seoul, 5Wonkwang University Medical School, Iksan, 6Chungnam National University College of Medicine, Daejeon, 7Keimyung University School of Medicine, Daegu, Korea Corresponding to Sunjoo Kim, E-mail: sjkim8239@hanmail.net Ann Clin Microbiol 2012;15(2):67-69. https://doi.org/10.5145/KJCM.2012.15.2.67Copyright © Korean Society of Clinical Microbiology. Abstract The authors calculated the number of blood cultures per 1,000 admitted patient days at seven university-affiliated hospitals in 2010, which ranged from 65 to 129 (mean 110). The number of blood cultures per 1,000 patient days could possibly be a good parameter for assessing the appropriateness of blood culture. (Korean J Clin Microbiol 2012;15:67-69) Keywords

Original article PDF Hyo Jin Kim1, Younghee Seo1, Wan Hee Kim1, Yangsoon Lee1, Hyukmin Lee2, Kyungwon Lee1, Yunsop Chong Department of Laboratory Medicine, 1Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 2Kwandong University College of Medicine, Incheon, Korea Corresponding to Hyukmin Lee, E-mail: hmlee.labmed@gmail.com Ann Clin Microbiol 2013;16(4):182-187. https://doi.org/10.5145/ACM.2013.16.4.182Copyright © Korean Society of Clinical Microbiology. Abstract Background: Antimicrobial resistance of Neisseria gonorrhoeae has become a serious problem worldwide, and ceftriaxone non-susceptible isolates have been recently reported from Japan and Europe. In this study, we evaluated the antimicrobial susceptibilities and molecular epidemiological characteristics of isolates from Korea in 2013. Methods: Sixty strains of N. gonorrhoeae were collected from Korean patients and prostitutes. Antimicrobial susceptibility was tested by the agar dilution and disk diffusion methods. N. gonorrhoeae multi-antigen sequence typing (NG-MAST) was performed in order to determine the molecular epidemiologic relatedness. Results: All of isolates were non-susceptible to penicillin

Original article PDF In Joo Cho1, Jisook Yim1, Yangsoon Lee1,2, Myung Sook Kim1, Youkyung Seo1, Hae-Sun Chung1, Dongeun Yong1, Seok Hoon Jeong1, Kyungwon Lee1, Yunsop Chong1 1Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 2Department of Laboratory Medicine, National Health Insurance Service Ilsan Hospital, Goyang, Korea Corresponding to Kyungwon Lee, E-mail: leekcp@yuhs.ac Ann Clin Microbiol 2013;16(1):45-51. https://doi.org/10.5145/ACM.2013.16.1.45Copyright © Korean Society of Clinical Microbiology. Abstract Background: Trends in the isolation of enteropathogenic bacteria may differ depending on environmental sanitation. The aims of this study were to determine trends in the isolation and antimicrobial resistance patterns of enteropathogenic bacteria over the last 10 years. Methods: We analyzed stool cultures of Salmonella spp., Shigella spp., Plesiomonas shigelloides, Yersinia spp., Vibrio spp., and Campylobacter spp. collected at Severance Hospital between 2001 and 2010. Antimicrobial susceptibility testing was performed using the disk diffusion method for nontyphoidal Salmonella (NTS)

Original article PDF Hae-Sun Chung1,2, Yangsoon Lee2, Eun Suk Park3, Dong Suk Lee3, Eun Jin Ha3, Myungsook Kim2, Dongeun Yong2, Seok Hoon Jeong2, Kyungwon Lee2,3, Yunsop Chong2 1Department of Laboratory Medicine, Ewha Womans University School of Medicine, 2Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, 3Department of Infection Control, Severance Hospital, Seoul, Korea Corresponding to Dongeun Yong, E-mail: deyong@yuhs.ac Ann Clin Microbiol 2014;17(2):29-34. https://doi.org/10.5145/ACM.2014.17.2.29Copyright © Korean Society of Clinical Microbiology. Abstract Background: Acinetobacter spp. is an important nosocomial pathogen for which increasing resistance to multiple antimicrobial agents has been observed. Prevalence of multidrug-resistant (MDR) Acinetobacter spp. in the intensive care unit (ICU) at a teaching hospital in Korea started to increase in 2008. The aim of this study was to determine the source of pathogen spread and to characterize the emerging strains at an early stage of outbreak. Methods: Samples from respiratory instruments

Original article PDF Ki Ho Hong Department of Laboratory Medicine, Seoul Medical Center, Seoul, Korea Corresponding to Ki Ho Hong, E-mail: kihohongmd@gmail.com Ann Clin Microbiol 2017;20(2):27-34. https://doi.org/10.5145/ACM.2017.20.2.27Copyright © Korean Society of Clinical Microbiology. Abstract As many emerging human infections are caused by viruses, laboratory-acquired viral infection will become more common. However, additional knowledges is needed, including actual incidence, disinfectant, and prevention. Although the general biosafety principles of viruses do not differ from those of other microorganisms, biosafety guidelines and programs are not immutable and could vary according to virus and laboratory environment. Most laboratory-acquired viral infections reported in the literature were caused by violation of biosafety principles. (Ann Clin Microbiol 2017;20:27-34) Keywords Laboratory infection, Occupational diseases, Virus diseases

Original article PDF Da Young Kang1,2, Jun Sung Hong1, Byeol Yi Park1,2, In-Ho Jang2, Dokyun Kim1, Hyukmin Lee1, Seok Hoon Jeong1 1Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 2Department of Clinical Pathology, Sangji University College of Health Science, Wonju, Korea Corresponding to Dokyun Kim, E-mail: kyun-sky@yuhs.ac Ann Clin Microbiol 2019;22(3):61-70. https://doi.org/10.5145/ACM.2019.22.3.61Copyright © Korean Society of Clinical Microbiology. Abstract Background: Antimicrobial resistant continues to pose a threat to public health. Therefore, rapid and accurate antimicrobial susceptibility testing is very important. The objectives of this study were to evaluate the performance of the MicroScan system (Beckman Coulter, USA) with newly developed Korean Antimicrobial Susceptibility Testing Panels (KSCM panels) for antimicrobial susceptibility testing (AST) against clinical isolates in South Korea. Methods: Three KSCM panels were designed in this study. For the performance evaluation, a total of 1,325 clinical isolates including 1,027 of Gram-negative bacilli

Original article Jun Sung Hong1*, Byeol Yi Park1*, Dokyun Kim1, Kunhan Kim1, Kyoung Hwa Lee2, Nan Hyoung Cho3, Seok Hoon Jeong1 1Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 2Division of Infectious Disease, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, 3Department of Infection Control, Gangnam Severance Hospital, Seoul, Korea. * These authors contributed equally to this work. Corresponding to Dokyun Kim, E-mail: kyunsky@yuhs.ac Ann Clin Microbiol 2020;23(2):81-92. https://doi.org/10.5145/ACM.2020.23.2.5Received on 23 October 2019, Revised on 6 January 2020, Accepted on 6 January 2020, Published on 20 June 2020.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The prevalence of carbapenemase-producing Enterobacteriaceae (CPE), especially the KPC-2-producing Klebisella pneumoniae, is rapidly increasing and becoming a menace to global public health. This study aims to present the molecular epidemiology of the KPC-2-producing K. pneumoniae isolates emerged in a tertiary hospital in South Korea

Original article Viga Rolly1*, Eun Jung Lee2*, Eun-Jeong Yoon3, Seok Hoon Jeong3 1Global Health Security, Graduate School of Public Health, Yonsei University, Seoul, 2Sangji University College of Science, Wonju, 3Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University, College of Medicine, Seoul, Korea *These authors contributed equally to this work. Corresponding to Eun-Jeong Yoon, E-mail: ejyoon@yuhs.ac Ann Clin Microbiol 2020;23(2):73-80. https://doi.org/10.5145/ACM.2020.23.2.4Received on 24 July 2019, Revised on 19 November 2019, Accepted on 19 November 2019, Published on 20 June 2020.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Ideal dose of the antimicrobials should be decided by considering their killing dynamics since sufficient elimination of the causative microorganisms is critical for proper antimicrobial treatment. In this study, the bactericidal activities of carbapenems by resistance mechanisms were assessed for carbapenem-resistant Pseudomonas aeruginosa. Methods: Minimal inhibition concentrations (MICs) of carbapenems were determined by broth dilution method and the resistance

Case report Jae-Seok Kim, Sung Hee Chung, Jung-Min Kim, Hyun Soo Kim, Han-Sung Kim, Wonkeun Song Department of Laboratory Medicine, Hallym University College of Medicine, Seoul, Korea Corresponding to Jae-Seok Kim, E-mail:  jaeseok@hallym.ac.kr Ann Clin Microbiol 2021;24(4):135-140. https://doi.org/10.5145/ACM.2021.24.4.4Received on 28 June 2021, Revised on 27 September 2021, Accepted on 11 November 2021, Published on 20 December 2021.Copyright © Korean Society of Clinical Microbiology. Abstract The application of whole genome sequencing on SARS-CoV-2 viral genome is essential for our understanding of the molecular epidemiology and spread of viruses in the community. The portable whole genome sequencer MinION (Oxford Nanopore Technologies, ONT, UK) could be feasibly used in a clinical microbiology laboratory without the need of vast resources or stringent operating conditions. We used the MinION sequencer to analyze the viral genome sequence of one SARS-CoV-2 strain. In June 2020, nasopharyngeal specimen from one patient was subjected to whole-genome analysis using the