Original article PDF Ji Hae Kang1, Il Kwon Bae2, Su Bong Kwon2, Seok Hoon Jeong2*, Jongwook Lee3, Wee Gyo Lee4, Jung Oak Kang5, Ji Young Ahn6, Seong Geun Hong7, Jong Hee Shin8, Young Uh9, Yeon Jun Park10, Eui-Chong Kim11, Kyungwon Lee12, Dongeun Yong12, and Gun Jo Woo13 Departments of Pediatrics1and Laboratory Medicine2, Kosin University College of Medicine, Busan; Department of Laboratory Medicine3, Keonyang University College of Medicine, Daejeon; Department of Laboratory Medicine4, Ajou University College of Medicine, Suwon; Department of Laboratory Medicine5, Hanyang University College of Medicine, Guri; Department of Laboratory Medicine6, Sooncheonhyang University College of Medicine, Gumi; Department of Laboratory Medicine7, Pochon Cha University College of Medicine, Sungnam; Department of Laboratory Medicine8, Chonnam National University Medical School, Gwangju; Department of Laboratory Medicine9, Yonsei University Wonju College of Medicine, Wonju; Department of Laboratory Medicine10, The Catholic University of Korea College of Medicine, Seoul; Department of Laboratory Medicine11, Seoul National University

Original article PDF Wee Gyo Lee1, Il Joong Park1, Ji Young Huh2, Eui-Chong Kim2, Kyungwon Lee3, Mi-Na Kim4, Chulhun L. Chang5, Sunjoo Kim6, Young Uh7, Insoo Rheem8, Gyoung Yim Ha9, Hye Soo Lee10 Department of Laboratory Medicine1, Ajou University School of Medicine, Suwon; Department of Laboratory Medicine2, Seoul National University College of Medicine; Department of Laboratory Medicine3, Yonsei University College of Medicine; Department of Laboratory Medicine4, University of Ulsan College of Medicine, and Asan Medical Center, Seoul; Department of Laboratory Medicine5, Pusan National Uinversity School of Medicine, Busan; Department of Laboratory Medicine6, Gyeongsang University College of Medicine, Jinju; Department of Laboratory Medicine7, Yonsei University Wonju College of Medicine, Wonju; Department of Laboratory Medicine8, Dankook University College of Medicine, Chunan; Department of Laboratory Medicine9, Dongguk University College of Medicine, Gyungju; and Department of Laboratory Medicine10, Chonbuk National University College of Medicine, Chonju, Korea Corresponding to Wee Gyo Lee, E-mail: weegyo@ajou.ac.kr Ann

Original article PDF Il Kwon Bae1, Yu Nae Lee1, Seok Hoon Jeong1, Kyungwon Lee2, Dongeun Yong2, Jongwook Lee3, Seong Geun Hong4, Eui-Chong Kim5, Yeon Jun Park6, Tae Yeal Choi7, Young Uh8, Jong Hee Shin9, Wee Gyo Lee10, Ji young Ahn11, Sung-Hee Lee12, Gun-Jo Woo13, Hyo-Sun Kwak13 Department of Laboratory Medicine1, Kosin University College of Medicine, Busan; Department of Laboratory Medicine2, Yonsei University College of Medicine, Seoul; Department of Laboratory Medicine3, Keonyang University College of Medicine, Daejeon; Department of Laboratory Medicine4, Pochon Cha University College of Medicine, Sungnam; Department of Laboratory Medicine5, Seoul National University College of Medicine; Department of Laboratory Medicine6, The Catholic University College of Medicine; Department of Laboratory Medicine7, Hanyang University College of Medicine, Seoul; Department of Laboratory Medicine8, Yonsei University Wonju College of Medicine9, Wonju; Department of Laboratory , Chonnam National University College of Medicine, Gwangju; Department of Laboratory Medicine10, Ajou University College of Medicine, Suwon; Department

Case report PDF Chang Ki Kim1 , Injoo Cho1 , Youn Hee Park1, Kyoung Ho Roh1, Dongeun Yong1,3,4, Kyungwon Lee1,3,4 , June Myung Kim2,3,4, Yunsop Chong1,3 Departments of Laboratory Medicine1, Internal Medicine2; Research Institute of Bacterial Resistance3; and Brain Korea 21 Project for Medical Sciences4, Yonsei University College of Medicine, Seoul, Korea Corresponding to Dongeun Yong, E-mail: deyong@yumc.yonsei.ac.kr Ann Clin Microbiol 2006;9(1):71-75.Copyright © Korean Society of Clinical Microbiology. Abstract Haemophilus aphrophilus is a facultative anaerobic, gram-negative coccobacillus or bacillus and its growth is stimulated by 5 to 10% CO2 . Most Haemophilus species require either exogenous X or V factor or both to grow, but H. aphrophilus can grow without these factors. H. aphrophilus rarely causes invasive infections such as endocarditis, septicemia, pneumonia and peritonitis in human. Two cases of infective endocarditis by H. aphrophilus have been reported in Korea. However, there has been no report of polymicrobial endocarditis by H. aphrophilus and other bacteria. We isolated H. aphrophilus and coagulase-negative staphylococci (CNS) from

Case report PDF Eun-Mi Koh1,3, Chang Ki Kim1,3, Myungsook Kim3, Shin-Moo Kim4, Seung-Woo Park2, Hyun Cheol Chung2, Dongeun Yong1,3, Kyungwon Lee1,3, Yunsop Chong1,3 Departments of 1Laboratory Medicine and 2Internal Medicine, and 3Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul; 4Department of Clinical Laboratory Science, Wonkwang Health Science College, Iksan, Korea Corresponding to Kyungwon Lee, E-mail: leekcp@yumc.yonsei.ac.kr Ann Clin Microbiol 2007;10(2):164-167.Copyright © Korean Society of Clinical Microbiology. Abstract Vibrio fluvialis is a haplophilic gram-negative bacterium normally found in coastal water and seafood and causes gastroenteritis. There have been a few reports on V. fluvialis gastroenteritis in Korea, but no previous report of isolation from blood. We isolated V. fluvialis from the blood of two patients undergoing cancer chemotherapy. (Korean J Clin Microbiol 2007;10:164-167) Keywords Vibrio fluvialis, Blood culture, Bacteremia

Original article PDF Hyukmin Lee1, Eun Mi Koh2, Myung Sook Kim2, Jong Hwa Yum2, Dongeun Yong2, Wee Gyo Lee3, Kyungwon Lee2, Yunsop Chong2 1Department of Laboratory Medicine, Kwandong University College of Medicine, Goyang; 2Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul; 3Department of Laboratory Medicine, Ajou University School of Medicine, Suwon, Korea Corresponding to Kyungwon Lee, E-mail: leekcp@yumc.yonsei.ac.kr Ann Clin Microbiol 2007;10(2):114-118.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Enterococcus gallinarum, an organism often found in the intestine, is intrinsically resistant to low level vancomycin, but some of them are highly resistant to vancomycin due to acquisition of vanA gene. We occasionally detected both vanA carrying E. gallinarum and E. faecalis or E. faecium in the same patients, suggesting transfer of the resistance gene from the latter. In this study, the structures of Tn1546-like elements in E. gallinarum, and E. faecalis or E. faecium from the same patients were compared to determine the clinical significance of the vanA genotype E. gallinarum isolates. Methods: Six

Original article PDF Hyukmin Lee1, Chang Ki Kim2, Jongwook Lee3, Sung-Hee Lee4, Ji Young Ahn5, Seong Geun Hong6, Yeon Jun Park7, Seok Hoon Jeong8, Eui-Chong Kim9, Wee Kyo Lee10, Young Uh11, Jong Hee Shin12, Tae Yeal Choi13, Hyo-Sun Kwak14, Kyungwon Lee2 Department of Laboratory Medicine, 1Kwandong University College of Medicine, Goyang; 2Yonsei University College of Medicine, Seoul; 3Keonyang University Medical College, Daejeon; 4Cheju Hanmaeum Hospital, Jeju; 5Sooncheonhyang University College of Medicine, Gumi; 6Pochon CHA University College of Medicine, Seongnam; 7 The Catholic University of Korea College of Medicine, Seoul; 8Kosin University College of Medicine, Busan; 9Seoul National University College of Medicine, Seoul; 10Ajou University College of Medicine, Suwon; 11Yonsei University Wonju College of Medicine, Wonju; 12Chonnam National Univeristy Medical School, Gwangju; 13Hanyang University College of Medicine, Seoul; 14Food Microbiology Division, Center for Food Safety Evaluation, Korea Food and Drug Administration, Seoul, Korea Corresponding to Kyungwon Lee, E-mail: leekcp@yumc.yonsei.ac.kr Ann Clin Microbiol 2007;10(1):59-69.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Emergence and spread of antimicrobial

Original article PDF Chang-Ki Kim1, Jong Hwa Yum2, Dongeun Yong3,4, Seok Hoon Jeong3,4, Kyungwon Lee3,4, Yunsop Chong3,4 1Korean Institute of Tuberculosis, The Korean National Tuberculosis Association, Seoul; 2Department of Biomedical Laboratory Science, Dong-Eui University College of Natural Science, Busan; 3Department of Laboratory Medicine and 4Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea Corresponding to Kyungwon Lee, E-mail: leekcp@yuhs.ac Ann Clin Microbiol 2008;11(2):90-97.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Clinical isolates of AmpC β-lactamase- producing Enterobacteriaceae were evaluated to determine the prevalence of CTX-M extended-spectrum β-lactamases (ESBLs) and their genetic environments. Methods: A total of 250 non-duplicate isolates of Eneterobacter aerogenes, E. cloacae, Citrobacter freundii, Serratia marcescens and Morganella morganii were collected at a Korean hospital. ESBL production was determined by double disk synergy test. For ESBL producers, bla genes were sequenced and blaCTX-Menvironment was characterized by PCR mapping and sequencing. Results: Among the 250 isolates 29 (11.6%) produced ESBL, and 14 of the 29 isolates produced CTX-M ESBLs, including CTX-M-9 by

Original article PDF Sue Jung Kim1, Heejung Kim1, Myung Sook Kim1, Eunmi Koh1, Chang-Ki Kim2, Seok Hoon Jeong1, Yunsop Chong1, Kyungwon Lee1 1Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine; 2Korean Institute of Tuberculosis, Seoul, Korea Corresponding to Kyungwon Lee, E-mail: leekcp@yuhs.ac Ann Clin Microbiol 2008;11(2):112-116.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Toxin immunoassay is widely used for rapid diagnosis of Clostridium difficile-associated diarrhea. The aim of this study was to evaluate the performance of Tox A/B Quik Chek test (TECHLAB, Blacksburg, VA, USA) compared to toxigenic culture. Methods: From September 2006 to August 2007, 959 stools were examined by Tox A/B Quik Chek test and toxigenic culture (C. difficile culture plus tcdB PCR using colonies obtained from culture). Results: Compared to the results of toxigenic culture, the sensitivity and specificity of Tox A/B Quik Chek test were 47.5% and 97.5%, respectively. Conclusion: The sensitivity