Ann Clin Microbiol 2020;23:135-148. Evaluation of the Performance of ASTA MicroIDSys, a Novel Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry System, in Identification of Bacterial Clinical Isolates Download table Reference identification (number of isolates) ASTA MicroIDSys, number of isolates   Bruker Biotyper, number of isolates Correctly identified Mis-ID Invalid-ID   Correctly identified Mis-ID Invalid-ID Genus level Species level   Genus level Species level Staphylococcus                   S. aureus (45) 45 45       45 45     S. epidermidis (42) 42 42       42 42     S. capitis (9) 9 9       9 9     S. haemolyticus (8) 8 8       8 8     S. lugdunensis (8) 8 8       8 8     S. caprae (6) 6 6       6 6     S. hominis (5) 5 5

Original article Changseung Liu1*, Eunjung Lee2*, Dokyun Kim1, Seok Hoon Jeong1 1Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 2Department of Clinical Pathology, Sangji University College of Science, Wonju, Korea *These authors contributed equally to this work. Corresponding to Dokyun Kim, E-mail: kyunsky@yuhs.ac Ann Clin Microbiol 2020;23(3):135-148. https://doi.org/10.5145/ACM.2020.23.3.3Received on 31 January 2020, Revised on 10 April 2020, Accepted on 10 April 2020, Published on 20 September 2020.Copyright © Korean Society of Clinical Microbiology. Abstract Background: We evaluated the performance of ASTA MicroIDSys (ASTA, Korea) and Bruker Biotyper (Bruker Daltonics, Germany) systems in the identification of bacterial isolates from clinical microbiology laboratory specimens during the study period. In addition, species for which the identification accuracy using MALDI-TOF MS systems was previously reported to be poor were also identified by comparing the MS results with those obtained using molecular identification. Methods: A total of

Original article Young-Suk Sohn1, Jung-Hyun Byun2, Young Ah Kim3, Dong-Chun Shin4, Kyungwon Lee1 1Department of Laboratory Medicine, Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 2Department of Laboratory Medicine, Gyeongsang National University Hospital, Gyeongsang National University College of Medicine, Jinju, 3Department of Laboratory Medicine, National Health Insurance Service Ilsan Hospital, Goyang, 4Department of Environmental Health Graduate School of Public Health, Yonsei University, Seoul, Korea Corresponding to Young Ah Kim, E-mail: yakim@nhimc.or.kr Ann Clin Microbiol 2020;23(1):33-43. https://doi.org/10.5145/ACM.2020.23.1.33Received on 8 July 2019, Revised on 23 August 2019, Accepted on 23 August 2019, Published on 20 March 2019.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The weather has well-documented effects on infectious disease and reports suggest that summer peaks in the incidences of gram-negative bacterial infections among hospitalized patients. We evaluated how season and temperature changes affect bloodstream infection (BSI) incidences of major pathogens to understand BSI trends with

Original article Hyunmi Cho1, Jong-Bae Kim2, Young Uh1 1Department of Laboratory Medicine, Wonju Severance Christian Hospital, Yonsei University Wonju College of Medicine, Wonju, 2Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Korea Corresponding to Young Uh, E-mail: u931018@yonsei.ac.kr Ann Clin Microbiol 2020;23(1):21-31. https://doi.org/10.5145/ACM.2020.23.1.21Received on 12 June 2019, Revised on 19 August 2019, Accepted on 19 August 2019, Published on 20 March 2019.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Rapid and accurate detection of Mycobacterium tuberculosis (MTB) is of primary importance for infection control and selection of anti-tuberculosis drugs. However, most clinical laboratories report MTB complex (MTC) without reporting MTB because MTC comprising MTB, Mycobacterium bovis, Mycobacterium africanum, Mycobacterium microti, Mycobacterium capraei and Mycobacterium pinnipedii have 99.9% similarity at the nucleotide level and identical 16S rRNA sequences. This study was conducted to analyze the species frequency of MTC isolates obtained from clinical specimen. Methods: Of

Ann Clin Microbiol 2024;27:93-130. Anisakidosis in humans and animals and detection of anisakid larvae in fish and cephalopods in Korea: a literature review (1971-2022) Download table Species of fish/cephalopods Korean common name Chun et al. (1968) [169] Lim (1975) [170] Kim et al. (1988) [171] Woo and Kim (2000) [125] Song et al. (2001) [177] Choi et al. (2009) [178] Choi et al. (2011) [182] Cho et al. (2012) [183] Locality surveyed West/ South Sea West/South sea Fish market in Seoul Jeju Island South Sea East/South/West Seas Fish market in Busan East/South/West Sea Acanthopagrus schlegeli 감성돔 ○ ● Apogon lineatus 열동가리돔 ○ Arctoscopus japonicus 도루묵 ● Argyrosomus argentatus 보구치 ● ● ● Bodianus oxycephalus 사랑놀래기 ● Branchiostegus japonicus 옥돔 ● Chelidonichthys sp. 성대 ● ● Chromis notata 자리돔 ● ● ○ Clupea palassii 청어 ● ● ● Clyptocephalus stellari 기름가자미 ● Cololobis saira 꽁치 ● ○ Conger myriaster 붕장어 ●

Original article PDF Eun Gyung Ko, M.D., Chang Jung Kim, M.D., Key Earn Lee, M.D., Jihyun Cho, M.D., and Young Hoe Moon, M.D. Department of Clinical Pathology, College of Medicine, Wonkwang University, Iksan, Korea Corresponding to Jihyun Cho Ann Clin Microbiol 1998;1(1):57-62.Copyright © Korean Society of Clinical Microbiology. Abstract Background: In developed countries, food-borne diseases have decreased and hospital laboratory have taken more simple method rather than complex enrichment-selective methods. But detection rate of pathogenic bacteria in stool culture was not so high. Methods: We mixed 4 pathogenic bacteria (S. typhi, S. flexneri, V. cholerae and Y. enterocolitica) with 3 stool specimens from healthy persons (for Y. enterocolitica, 5 specimens) and inoculated directly or after enrichment (10^5 bacteria/plate). After proper incubation, we counted suspected colonies and calculated true positive rate after identification of each colonies. Results: For S. typhi, in the case of direct innoculation on the MacConkey, XLD and SS agar, positive rate of selected

Original article PDF Young Uh, In Ho Jang, Gyu Yel Hwang, Kap Jun Yoon and Hyung-Hoan Lee* Department of Clinical Pathology, Yonsei University Wonju College of Medicine, Wonju, Korea, and Department of Biology, Kon-Kuk University, Seoul, Korea* Corresponding to Young Uh, E-mail: u931018@wonju.yonsei.ac.kr Ann Clin Microbiol 1999;2(1):58-63.Copyright © Korean Society of Clinical Microbiology. Abstract Background：The accurate and rapid identification of enterococci can provide clinician’s decision making of antimicrobial therapy because enterococci are usually multiresistant to commonly used antimicrobial agents and antimicrobial resistance patterns are different according to enterococcal species. Accuracy of identification system depends mainly on data base such as positive rate of biochemical reactions, relative frequency of occurrence of biotype, and isolation frequency of microorganisms. The purpose of this study was to analyze the isolation rate and biotype frequency of enterococci isolated from clinical specimens. Methods：We used a simplified identification system for the identification of the enterococci from clinical