Original article PDF Seung Hwan Oh1,2, Young Jin Kim1, Seung Kyu Park2, Sang Hyun Hwang1, Hyung Hoi Kim1, Eun Yup Lee1, Chulhun L. Chang1,3 1Department of Laboratory Medicine, Pusan National University School of Medicine, Busan; 2National Masan Hospital, Masan; 3Korean Institute of Tuberculosis, Seoul, Korea Corresponding to Chulhun L. Chang, E-mail: cchl@pusan.ac.kr Ann Clin Microbiol 2008;11(1):43-48.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The purposes of the current study were to evaluate the concordant rates of anti-mycobacterial drug susceptibility test (DST) results in different solid media performed in different institutes, and to determine reliable susceptible testing methods. Methods: One hundred and twenty two Mycobacterium tuberculosis strains were isolated from patients in A Hospital in 2005. DSTs were performed by the absolute concentration method using Löwenstein Jensen medium in both A Hospital (method A-1) and B Institute (method B-1) and by the proportion method using Middlebrook 7H10 agar in B Institute (method B-2). Nine

Original article PDF Young-Sook Cho1, Young-Hoon Kim1, Kyung-Hee Lee1, Hye-Sun Jang1, Kyung-Ah Hwang1, Yoo-Li Kim2, Hyun-Young Chi1 1Samkwang Medical Laboratories, 2Biosewoom Institute of Bioscience & Biotechnology, Seoul, Korea Corresponding to Hyun-Young Chi, E-mail: chumgang@smlab.co.kr Ann Clin Microbiol 2009;12(2):72-77.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Hepatitis C virus (HCV) RNA quantification is necessary for predicting the therapeutic response and assessing treatment results in patients with chronic HCV infection. Recently, real-time PCR technology for HCV RNA quantification displayed good linearity within the dynamic range. Thus, it is gradually replacing branched-DNA (bDNA) and PCR- hybridization assays. In this study, we evaluated the performance of the Real-QTM HCV quantification kit (biosewoom. Inc., Seoul, Korea) developed in Korea. Methods: We evaluated the HCV quantification kit for detection limit, specificity, linearity, accuracy, and recovery rate of HCV RNA standard material. The results were analyzed for a correlation with those of Cobas Amplicor HCV Monitor 2.0. Results:

Original article PDF Young-Eun Kim, Hee Jae Huh, Yu-Yean Hwang, Nam Yong Lee Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Koreaa Corresponding to Nam Yong Lee, E-mail: micro.lee@samsung.com Ann Clin Microbiol 2013;16(3):134-139. https://doi.org/10.5145/ACM.2013.16.3.134Copyright © Korean Society of Clinical Microbiology. Abstract Background: Due to a reduction in the number of parasite infections, attention paid to the importance of intestinal parasites has decreased. However, intestinal parasite infections remain ubiquitous and have reappeared as a growing problem in recent decades due to changing lifestyles such as increased overseas travel. In this study, we evaluated trends in intestinal parasite infection using stool examination in a single institute.  Methods: From January 2003 to December 2012, we reviewed all stool examination results performed at Samsung Medical Center. Fecal examinations were performed by formalin-ether sedimentation.  Results: A total 429,866 stool examinations were performed resulting in 14,672 cases with positive

Original article PDF Jae-Seok Kim1, Tae-Jung Sung2, Hong Kyu Park2, Ji Young Park1, Hyoun Chan Cho1, Il Tae Hwang2, Hae-Ran Lee2 Departments of 1Laboratory Medicine, 2Pediatrics, Hallym University College of Medicine, Seoul, Korea Corresponding to Hae-Ran Lee, E-mail: drran@hallym.or.kr Ann Clin Microbiol 2014;17(3):73-79. https://doi.org/10.5145/ACM.2014.17.3.73Copyright © Korean Society of Clinical Microbiology. Abstract Background: The intestinal microflora varies according to the factors such as age, diet and environment. It is debated whether the changes of microbiota after birth are associated with atopic disease. The purpose of this study was to investigate colonization rates of some intestinal microflora during the initial 9 months after birth, and their association with the development of atopy. Methods: Stool specimens were collected at 1, 3, 7 days and at 1, 2, 4, 6, 9 months after birth, and Escherichia coli, Lactobacillus, Bifidobacterium, Staphylococcus aureus were cultured with selective media. Diagnosis for atopy was accomplished via clinical history

Original article PDF Wonkeun Song1, Gilsung Yoo2, Gyu Yul Hwang2, Young Uh2 1Department of Laboratory Medicine, Hallym University College of Medicine, Seoul, 2Department of Laboratory Medicine, Wonju Severance Christian Hospital, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Young Uh, E-mail: u931018@yonsei.ac.kr Ann Clin Microbiol 2016;19(3):59-64. https://doi.org/10.5145/ACM.2016.19.3.59Copyright © Korean Society of Clinical Microbiology. Abstract Background: Extended-spectrum β-lactamase (ESBL)- producing Enterobacteriaceae are resistant to most β-lactam antibiotics except carbapenems. In recent years, infrequently isolated Enterobacteriaceae that produce carbapenemase pose a serious threat in the selection of appropriate therapeutic antibiotics. The rapid detection method of carbapenemase-producing Enterobacteriaceae (CPE) is necessary to prevent the spread of CPE into healthcare facilities. Methods: One hundred clinical Enterobacteriaceae isolates (Klebsiella pneumoniae 40, Escherichia coli 40, others 20) showing susceptibility to carbapenems and positivity in the CLSI ESBL phenotypic test from November 2015 to March 2016 and 59 stocked Enterobacteriaceae isolates harboring resistance genes producing

Original article PDF Sunyoung Ahn1, Ji Yeon Sung1, Hyunsoo Kim2, Myung Sook Kim1, Younjee Hwang3, Sori Jong1, Younghee Seo1, Eunjin Ha4, Eun Suk Park4, Jun Yong Choi4,5, Dongeun Yong1,4, Kyungwon Lee1 1Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, 2Department of Laboratory Medicine, National Police Hospital, 3Brain Korea 21 PLUS Project for Medical Science, Yonsei University, 4Department of Infection Control, Severance Hospital, 5Division of Infectious Diseases, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea Corresponding to Ji Yeon Sung, E-mail: jysung80@yuhs.ac Ann Clin Microbiol 2016;19(2):39-47. https://doi.org/10.5145/ACM.2016.19.2.39Copyright © Korean Society of Clinical Microbiology. Abstract Background: Carbapenemase-producing Enterobacteriaceae (CPE) has been increasingly reported worldwide in the past 10 years, which is an important infection control concern. Since the epidemiology and characteristics of these CPEs vary according to institutes, we aimed to characterize CPEs in a university hospital during the recent 4 years.

Original article PDF Si Hyun Kim1*, Eun Hee Park2*, In Yeong Hwang2, Hyukmin Lee3, Sae Am Song4, Miae Lee5, Seungok Lee6, Soo Young Kim6, Jin Ju Kim7, Jong Hee Shin8, Seong Geun Hong9, Kyeong Seob Shin10, Sunjoo Kim11, Nam Hee Ryoo12, Woonhyoung Lee13, Sook Jin Jang14, Jeong Hwan Shin4,15 1Department of Clinical Laboratory Science, Semyung University, Jecheon, 2Department of Infectious Disease, Busan Institute of Health and Environment, Busan, 3Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, 4Department of Laboratory Medicine, Inje University College of Medicine, Busan, 5Department of Laboratory Medicine, Ewha Womans University College of Medicine, Seoul, 6Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Seoul, 7Department of Laboratory Medicine, Inha University College of Medicine, Incheon, 8Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, 9Department of Laboratory Medicine, CHA Bundang Medical Center, CHA University, Seongnam, 10Department of Laboratory Medicine, Chungbuk National University

Original article Chorong Hahm1,2, Min-Kyung So1, Hae-Sun Chung1, Miae Lee1 1Department of Laboratory Medicine, Ewha Womans University College of Medicine, Seoul, 2Eone Laboratories, Seoul, Korea. Corresponding to Miae Lee, E-mail: miae@ewha.ac.kr  Ann Clin Microbiol 2020;23(2):105-116. https://doi.org/10.5145/ACM.2020.23.2.7Received on 5 March 2020, Revised on 28 April 2020, Accepted on 14 May 2020, Published on 20 June 2020.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The AdvanSure TB/NTM plus real-time PCR (AdvanSure plus PCR; LG Chem., Korea) assay has been developed to increase the diagnostic sensitivity of nontuberculous mycobacteria (NTM) compared with the currently used the AdvanSure TB/NTM real-time PCR (AdvanSure PCR; LG Chem., Korea) assay. In this study, we aimed to evaluate the performance of the AdvanSure plus PCR comparing the results with mycobacterial culture and the AdvanSure PCR. Methods: Patients (n=199) with suspected NTM or Mycobacterium tuberculosis complex (MTC) were tested using AdvanSure plus PCR, AdvanSure PCR, acid-fast bacilli staining,

Original article Jooyoung Cho1,2*, Jung-Hyun Byun1,3,4*, Sang-Guk Lee1, Kyeongjin Oh1, Beomchan Jeon1, Dongeun Yong1,3, Jeong-Ho Kim1,5 1Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, 2Department of Laboratory Medicine, Wonju Severance Christian Hospital, Yonsei University Wonju College of Medicine, Wonju, 3Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 4Department of Laboratory Medicine, Gyeongsang National University Hospital, Gyeongsang National University College of Medicine, Jinju, 5Department of Laboratory Medicine, Yongin Severance Hospital, Yonsei University College of Medicine, Yongin, Korea *These authors contributed equally to this work. Corresponding to Dongeun Yong, E-mail: deyong@yuhs.ac; Jeong-Ho Kim, E-mail: jeongho@yuhs.ac Ann Clin Microbiol 2021;24(3):83-96. https://doi.org/10.5145/ACM.2021.24.3.3Received on 1 April 2021, Revised on 15 July 2021, Accepted on 19 July 2021, Published on 20 September 2021.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Although urine culture is considered a reference standard for the diagnosis of urinary tract infection (UTI), it is