Case report PDF Mi Yeon Choi, M.D., Seong Soo Jang, M.D., Jung Oak Kang, M.D., and Myung Ju Ahn, M.D. Department of Clinical Pathology, and Internal Medicine*, College of Medicine, Hanyang University, Seoul, Korea Corresponding to Jung Oak Kang Ann Clin Microbiol 1998;1(1):104-108.Copyright © Korean Society of Clinical Microbiology. Abstract The major cause of pseudomembranous colitis is known to be Clostridium difficile (C. difficile). There are few reports that Clostridium species other than C. difficile has caused pseudomembranous colitis. We report a case of pseudomembranous colitis caused by Clostridium glycolicum (C. glycolicum). A 47-year-old woman who had operational history for rectal cancer 3 months ago, was readmitted with diarrhea of 3 days duration. Seven weeks before admission, she had received ornidazole and ceftriaxone due to diarrhea and abdominal pain, and her symptoms were improved. She had received additional radiation therapy for rectal cancer during six weeks before the recent onset of diarrhea. On admission, she complained of watery

Original article PDF Chulhun L. Chang, M.D., Dae Young Seo, M.D.,* Tae Hee Park, M.D., Jeong Seon Park, M.D., and Weon Joo Hwang, M.T. Department of Clinical Pathology, Pusan National University Hospital, Pusan; Department of Clinical Pathology,* Dong-A University Hospital, Pusan, Korea Corresponding to Chulhun L. Chang, E-mail: cchl@hyowon.cc.pusan.ac.kr Ann Clin Microbiol 2001;4(1):40-44.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Mycobacterial false-positive cultures have rarely been recognized in Korea, even though the rate of false-positive cultures of Mycobaterium tuberculosis has ranged from 0.4% to 4.0%. We estimated the false-positive rates by the review of medical records from whom mycobacterial cultures were requested, retrospeaively, after a bout of false-positive cultures was discovered in specimens treated in a single day. Methods : Of the total 2,245 specimens, including 337 positive cultures of mycobacteria, during the period of January and June 1999, seventy-two specimens that showed colonies less than or equal to 5 colonies

Original article PDF Jong Hee Shin, M.D., Chang Jae Lee, M.D., Jee Yeon Lee, M.D., Jeong Won Song, M.D., Seong Jung Kee, M.D., Soon Pal Suh, M.D., and Dong Wook Ryang, M.D. Department of Clinical Pathology, Chonnam National University Medical School, Kwangju, Korea Corresponding to Jong Hee Shin, E-mail: shinjh@chonnam.ac.kr Ann Clin Microbiol 2001;4(1):33-39.Copyright © Korean Society of Clinical Microbiology. Abstract Background : Aspergillus species are second only to Candida species as the most commonly isolated fungi from clinical specimens. As well as the identification of the Aspergillus species, it has been necessary for epidemiological studies to differentiate between strains of the same species. We performed genotypic identification and characterization of species and strains within the genus Aspergillus by using RAPD. Methods : A total of 63 clinical strains of Aspergillus species (including 21 A. fumigatus, 12 A. flavus, 12 A. niger, 12 A. terreus, 3 A. nidulans, and 3 A. sydowii) from 63 patients was analyzed. For RAPD alanysis, M13 primer (5’GAGGGTGGCGGTTCT3’) and five random 10-mer

Case report PDF Gyung Tae Chung, Do Hyun Kang, Cheon Kwon Yoo, Jong Hyun Choi* and Won Keun Seong Research Center for Pathogen Control, Department of Bacteriology, National Institute of Health, Seoul, Daegu Metropolitan City*, Korea Corresponding to Gyung Tae Chung, E-mail: gtchung@nih.go.kr Ann Clin Microbiol 2003;6(2):160-163.Copyright © Korean Society of Clinical Microbiology. Abstract Botulism is a rare neuroparalytic disease caused by neurotoxins of Clostridium species. A tenyear-old girl and her mother were admitted to a hospital with symptoms of progressive dizziness, blurred vision, slurred speech, constipation and difficulty in swallowing. These characteristic manifestations and clinical course prompted an examination of the possibility of botulism. Mouse bioassay performed with mother’s stool demonstrated type A botulinum toxin and culture of the mother’s stool was positive for Clostridium botulinum type A. This is the first case of botulism in Korea. (Korean J Clin Microbiol 2003;6(2):160-163) Keywords Botulism, Neurotoxin, Clostridium botulinum

Original article PDF Seong Geun Hong1, Sunjoo Kim2, Seok Hoon Jeong3, Chulhun L. Chang4, Sung Ran Cho5, Ji Young Ahn5, Jong Hee Shin6, Hye Soo Lee7, Won Keun Song8, Young Uh9, Jong Hwa Yum10, Dongeun Yong10, Kyungwon Lee10, Yunsop Chong10 Department of Laboratory Medicine, Coll. of Med., Pochon CHA Univ.1, Gyeongsang Nat. Univ. School of Med.2, Coll. of Med. Kosin Univ.3, Pusan Nat. Univ. Coll. of Med.4, Sooncheonhyang Univ. Coll. of Med.5, Chonnam Nat. Univ. Med. School6, Chonbuk Univ. Med. School7, Hanllym Univ. Coll. of Med.8, Yonsei Univ. Wonju Coll. of Med.9, Yonsei Univ. Coll. of Med.10 Corresponding to Kyungwon Lee, E-mail: leekcp@yumc.yonsei.ac.kr Ann Clin Microbiol 2003;6(2):149-155.Copyright © Korean Society of Clinical Microbiology. Abstract Background：Increase in extended-spectrum β-lactamase(ESBL)-producing Escherichia coli and Klebsiella pneumoniae have been reported in Korea. The aim of this study was to determine the nationwide prevalence of ESBL-producing E. coli and K. pneumoniae, and to investigate the types of ESBLs. Methods：A total of 2,221 E. coli and

Original article PDF Seong Geun Hong1, Dongeun Yong2, Kyungwon Lee2, Eui-Chong Kim3, Wee Kyo Lee4, Seok Hoon Jeong5, Won Keun Song6, Yeon Jun Park7, Mi-Na Kim8, Young Uh9, Jong Hee Shin10, Jongwook Lee11, Ji Young Ahn12, Sun Wha Lee13, Jae Seok Kim5, Hee Bong Shin1 Departments of Laboratory Medicine, Coll. of Med., Pochon CHA Univ.1, Yonsei Univ. Coll. of Med.2 Seoul Nat. Univ. Coll. of Med.3, Ajou Univ. of Med.4, Coll. of Med. Kosin Univ.5, Hanllym Univ. Coll. of Med.6 The Catholic Univ. of Kor.7, Univ. of Ulsan Med. Coll. and Asan Med. Center8, Yonsei Univ. Wonju Coll. of Med.9 Chonnam Nat. Univ. Med. School10, Keonyang Univ. Med. Coll.11 Sooncheonhyang Univ. Coll. of Med.12; Neodin Med. Res. Center13 Corresponding to Kyungwon Lee, E-mail: leekcp@yumc.yonsei.ac.kr Ann Clin Microbiol 2003;6(1):29-36.Copyright © Korean Society of Clinical Microbiology. Abstract Background: A rapid increase of antimicrobial-resistant bacteria has become a serious problem in many countries. The aim of this

Original article PDF Seong Geun Hong1, Jongwook Lee2, Dongeun Yong3, Eui-Chong Kim4, Seok Hoon Jeong5, Yeon Jun Park6, Tae Yeal Choi7, Young Uh8, Jong Hee Shin9, Wee Kyo Lee10, Ji Young Ahn11, Sung-Hee Lee12, Gun-Jo Woo13, and Kyungwon Lee3 Departments of Laboratory Medicine, Coll. of Med., Pochon CHA Univ.1; Keonyang Univ. Med. Coll.2; Yonsei Univ. Coll. of Med.3; Seoul Nat. Univ. Coll. of Med.4; Coll. of Med. Kosin Univ.5; The Catholic Univ. of Kor.6; Hanyang Univ. Coll. of Med.7; Yonsei Univ. Wonju Coll. of Med.8; Chonnam Nat. Univ. Med. School9; Ajou Univ. Coll. of Med.10; Sooncheonhyang Univ. Coll. of Med.11; Cheju Hanmaeum Hospital12; Food Microbiology Division, Center for Food Safety Evaluation, Korea Food and Drug Administration13 Corresponding to Kyungwon Lee, E-mail: leekcp@yumc.yonsei.ac.kr Ann Clin Microbiol 2004;7(2):171-177.Copyright © Korean Society of Clinical Microbiology. Abstract Background : A rapid increase in antimicrobial-resistant bacteria has become a serious problem in Korea. Moreover, the

Original article PDF Joseph Jeong1, Seon Ho Lee1, Ue-Suk Jeong1, Chulhun L. Chang2, Sung-Ryul Kim1 Department of Laboratory Medicine1, Ulsan University Hospital, Ulsan, Korea; Department of Laboratory Medicine2, College of Medicine, Pusan National University, Busan, Korea Corresponding to Sung-Ryul Kim, E-mail: 690519@hitel.net Ann Clin Microbiol 2004;7(2):148-155.Copyright © Korean Society of Clinical Microbiology. Abstract Background : As tuberculous and nontuberculous mycobacterial infections are increasing, it is very important to differentiate the myobacterial species. High performance liquid chromatography (HPLC) method has been proven to be a useful technique for the identification of mycobacteria. The purpose of this study was to investigate the identification rate using HPLC and to know nontuberculous mycobacterial distribution in Ulsan University Hospital. Method : Mycobacteria grew in 959 clinical specimens, which were analyzed by HPLC, and their distribution was reviewed by retrospective studies. Results : The patterns of HPLC were divided into single, double, and triple cluster groups

Original article PDF Ue Suk Joung, Joseph Jeong, Seon Ho Lee, Sung-Ryul Kim Department of Laboratory Medicine, Ulsan University Hospital, University of Ulsan College of Medicine, Ulsan, Korea Corresponding to Sung-Ryul Kim, E-mail: srkimuuh@hitel.net Ann Clin Microbiol 2004;7(2):135-138.Copyright © Korean Society of Clinical Microbiology. Abstract Background : Mycobacterial disease is still greatly concerned in the developing and industrialized countries. Ogawa media has been used to diagnose mycobacterial disease in Korea in spite of a low sensitivity and long incubation time. Mycobacterium Growth Indicator Tube (MGIT) 960 system has been developed to overcome the pitfalls of Ogawa media. So, we investigated improvement in dectection rate and the detection time of mycobacteria using the MGIT 960 system along with 3% Ogawa media. Methods : A total of 8,045 clinical specimens referred to the department of laboratory medicine in Ulsan University Hospital from January in 2001 to June in 2002 were cultured for

Original article PDF Ue Suk Joung, Seon Ho Lee, Joseph Jeong, Sung-Ryul Kim Department of Laboratory Medicine, Ulsan University Hospital, University of Ulsan College of Medicine, Ulsan, Korea Corresponding to Joseph Jeong, E-mail: 690519@hitel.net Ann Clin Microbiol 2004;7(2):130-134.Copyright © Korean Society of Clinical Microbiology. Abstract Background : Non-O157 human isolates among enterohemorrhagic Escherichia coli (EHEC) serogroup have been reported with increasing frequency in recent years; the serotype O26 is the most common among the non-O157 isolates. We performed serotyping of E. coli isolates with O157, O26, and O111 antisera at Ulsan University Hospital and identified 27 isolates of O26. The purpose of this study was to investigate the clinical significance of E. coli O26 isolates. Methods : During the 24-month period from January 2002 to December 2003, E. coli isolates were serotyped when requested by the physician because of bloody diarrhea or when blood was noted in the stool specimen at the laboratory. The isolates were identified biochemically