Case report PDF Hyun Yong Hwang, M.D., Seok Hoon Jeong, M.D., Hark Rim*, M.D., Mi Hyang Kim, M.D., Tae Jeon Jeong, M.T., Byeong Gil Choi, M.T. Departments of Clinical Pathology and Internal Medicine*, Kosin University College of Medicine, Pusan, Korea Corresponding to Hyun Yong Hwang Ann Clin Microbiol 1998;1(1):113-116.Copyright © Korean Society of Clinical Microbiology. Abstract A 60-year-old male with continuous ambulatory peritoneal dialysis was admitted because of abdominal discomfort and turbid dialysate. He had a history of chronic renal failure due to diabetic nephropathy. His WBC count of peripheral blood was 8,500/mm³ (neutrophil 92%), and that of dialysate was 1,400/mm³ (polymorphonuclear leukocyte 69%, lymphocyte 31%). Pure growth of Leclercia adecarboxylata was isolated from dialysate. The L. adecarboxylata isolate was susceptible to ampicillin, ampicillin/sulbactam, cephalothin, cefoperazone, cefoxitin, cefotaxime, ceftazidime, aztreonam, imipenem, gentamicin, tobramycin, amikacin, tetracycline, trimethoprim-sulfamethoxazole and ciprofloxacin. Cephalothin & amikacin were added into dialysate, and his clinical symptoms and turbidity of dialysate were

Case report PDF Soo Youn Lee, M.D., Jung Soo Lee, M.D., Mi Ae Lee, M.D., Wha Soon Chung, M.D. and Seon Ju Kim, M.D.* Department of Clinical Pathology, College of Medicine, Ewha Womans University, Seoul, Korea and Department of Clinical Pathology, Gyeungsang National University School of Medicine, Chinju, Korea* Corresponding to Mi Ae Lee Ann Clin Microbiol 1998;1(1):109-112.Copyright © Korean Society of Clinical Microbiology. Abstract The group A streptococcus is capable of producing exotoxins that have been linked to a toxic shock-like syndrome. Streptococcal toxic shock syndrome is a rapidly progressive associated with injury to multiple organ systems and a 30-60% mortality rate. These cases are very rare in Korea. We present a case of 32-year-old pregnant woman who developed streptococcal toxic shock syndrome following intrauterine fetal death. She manifested hypotension, shock, increased level of creatinine, disseminated intravascular coagulopathy, liver impairment, renal failure, pulmonary edema and adult respiratory distress syndrome.

Case report PDF Jang Su Kim, M.D., Chang Kyu Lee, M.D., In Bum Suh, M.D., Hyeun Ah Lee, Young Kee Kim, M.D. and Kap No Lee, M.D. Department of Clinical Pathology, Korea University Medical College, Seoul, Korea Corresponding to Chang Kyu Lee Ann Clin Microbiol 1999;2(2):216-219.Copyright © Korean Society of Clinical Microbiology. Abstract Purpura fulminans is a potentially disabling and life-threatening disorder characterized by acute onset of progressive cutaneous hemorrhage and necrosis on distal extremities, and disseminated intravascular coagulopathy. We experienced a case of purpura fulminans due to Streptococcus pneumoniae. A 42-year-old woman presented with skin petechiae, ecchymosis and gangrene on distal extremities with laboratory evidence of DIC. The latex agglutination test with CSF was positive at Streptococcus pneumoniae. To our knowledge, this is the first report of purpura fulminans caused by Streptococcus pneumoniae in Korea. (Korean J Clin Microbiol 1999;2:216-219) Keywords Purpura fulminans, Streptococcus pneumoniae, Septicemia

Original article PDF Sook Won Ryu, M.D., Chang Kyu Lee, M.D., Chang Su Kim M.D., Do Hyun Lee, M.T., Young Kee Kim, M.D. and Kap No Lee, M.D. Department of Clinical Pathology, College of Medicine, Korea University, Seoul, Korea Corresponding to Chang Kyu Lee Ann Clin Microbiol 1999;2(2):199-206.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The incidence of reported nontyphoidal Salmonellosis has increased during last decade in Korea. Salmonella typhimurium and Salmonella enteritidis are two major serotypes in nontyphoidal Salmonella. To determine the nature of potential outbreak S. typhimurium infection in a community, we retrospectively evaluated clinical and epidemiologic features of S. typhimurium infections and performed pulsed-field gel electrophoresis (PFGE) to investigate a genetic relatedness of S. typhimurium isolated in Guro Hospital. Methods: From May 1998 to April 1999, a total of 20 S. typhimurium strains were isolated from 18 patients. PFGE patterns were analyzed for 20 S. typhimurium strains. Clinical and epidemiological features were evaluated from their medical records. Results:

Original article PDF Jae Lim Chung, M.D., Young Ah Kim, M.D., Hee Bong Shin, M.D., Jeong Won Shin, M.D., Kyungwon Lee, M.D., Yunsop Chong, Ph.D., Jang Hyeon Park, Ph.D.* and Won Bae Kim, Ph.D.* Department of Clinical Pathology and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Research Laboratories, Dong-A Pharm. Co., Ltd., Yongin, Kyunggi-do, Korea* Corresponding to Kyungwon Lee Ann Clin Microbiol 1999;2(2):182-193.Copyright © Korean Society of Clinical Microbiology. Abstract Background: β-lactam antibiotics are one of the most frequently used antimicrobial agents. However, with the increase of β-lactamase-producing bacteria, penicillins and 1st generation cephalosporins have become less useful. Cefatrizine and clavulanic acid combination (CTCA) was developed to restore the activity. The aim of this study was to determine the activities of CTCA against major recent clinical isolates. Methods: Aerobic and anaerobic bacteria tested were isolated from clinical specimens in Severance Hospital during 1996 to 1999. Antimicrobial

Original article PDF Eui-Chong Kim*1,2, Jung-Hee Lee2, Hyun-Jin Jung1, and Young-Joon Lee1 Department of Clinical Pathology, Seoul National University College of Medicine1; Clinical Research Institute, Seoul National University Hospital2, Seoul, Korea Corresponding to Eui-Chong Kim, E-mail: euichong@plaza.snu.ac.kr Ann Clin Microbiol 1999;2(2):172-176.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Hand-foot-mouth disease (HFMD) is mainly caused by the infection of coxsackievirus A16. But recently several epidemics of HFMD with meningitis or myocarditis due to enterovirus 71 have been reported in Southeast Asia. It was necessary that the possibility of enterovirus 71 epidemic in Korea should be ruled out. This study was designed for the determination of causative agents of HFMD in Cheju province in the spring of 1998. Methods: Serum specimens were collected from 45 pediatric patients with HFMD at Cheju Hankook Hospital in March and April, 1998. Virus isolation was performed with RD cell culture through up to three passages.

Original article PDF Hyukmin Lee, M.D., Young Ah Kim, M.D., Kwang Il Park, M.D., Kyungwon Lee, M.D. and Yunsop Chong, Ph.D. Department of Clinical Pathology and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea Corresponding to Kyungwon Lee Ann Clin Microbiol 1999;2(1):77-81.Copyright © Korean Society of Clinical Microbiology. Abstract Background：Clostridium difficile causes antibiotic-associated diarrhea or pseudomembranous colitis by producing of toxins in patients treated with antimicrobial agents. Stool cultures for C. difficile and tests for the presence of its toxin are the most widely used methods for the diagnosis of infection. The aim of this study was to determine the usefulness of polymerase chain reaction for the detection of toxin B gene from C. difficile isolates. Methods：In this study, 85 strains of C. difficile were used, which were isolated from stool specimens of patients with suspected antibiotic-associated diarrhea or pseudomembranous colitis from 1987 to 1994 using cefoxitin-cycloserine-fructose agar. DNA of the C. difficile isolates was

Case report PDF Ji Soo Kim, M.D., Soo Yeon Park, M.D., Yeoung Chul Kil, M.T., Hee Joo Lee, M.D., and Jin Tae Suh, M.D. Department of Clinical Pathology, College of Medicine, Kyunghee University, Seoul, Korea Corresponding to Ji Soo Kim, E-mail: jakesu@yahoo.co.kr Ann Clin Microbiol 2000;3(2):147-150.Copyright © Korean Society of Clinical Microbiology. Abstract V. parahaemolyticus or V. alginolyticus infections are usually associated with consumption of raw or undercooked shellfish, contaminated food, and exposure of wounds to warm seawater. V. parahaemolyticus causes gastroenteritis (the most common syndrome), wound infections, and septicemia. V. alginolyticus occasionally causes extraintestinal infections in humans. So far, the authors have not found the report of V. parahaemolyticus and V. alginolyticus isolation from a patient. So, we report a case of concurrent isolation of V. parahaemolyticus and V. alginolyticus from a patient who had a history of intestinal diarrhea and vomiting. (Korean J Clin Microbiol 2000;3:147-150) Keywords V. parahaemolyticus, V. alginolyticus, stool culture