Original article PDF Young Uh, Soon Duk Park, Hyun Mi Cho, Hyeun Gyeo Lee, An Suk Jeoung, Mi Kyung Lee, and Kap Jun Yoon Department of Clinical Pathology, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Young Uh, E-mail: u931018@wonju.yonsei.ac.kr Ann Clin Microbiol 2002;5(1):42-46.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The test for hippurate hydrolysis is critical for differentiation of C. jejuni and other thermophilic Campylobacter species. So, we evaluated the disk method for detection of hippurate hydrolysis by C. jejuni. Methods: Twenty-eight Campylobacter species isolated from stool culture were simultaneously tested with disk method for detection of hippurate hydrolysis and polymerase chain reaction (PCR) for hippuricase specific gene. Disk method was tested with difference in incubation time (2 hours vs. 4 hours), hippurate concentration (1%, 2%, and 4%), amount of ninhydrin (50 μL vs. 100 μL), and inoculation method (colony vs. suspension of organism adjusted by turbidity), finally, 24 types of disk

Original article PDF Young Uh, Hyun Mi Cho, In Ho Jang, Kap Jun Yoon, and Dong Min Seo* Departments of Clinical Pathology and Medical Information Development*, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Young Uh, E-mail: u931018@wonju.yonsei.ac.kr Ann Clin Microbiol 2002;5(1):26-34.Copyright © Korean Society of Clinical Microbiology. Abstract Background:The accurate and rapid identification (ID) of nonfermentative gram-negative bacilli (NFB) is essential for diagnostic and therapeutic purposes and for epidemiologic studies of hospital infections. Commercial identification systems of NFB are easy to use but too expensive. The aim of the study was to develop a simple system for the identification of NFB species which are frequently isolated from clinical specimens. Methods:Eighteen biochemical tests used in NFB microplate ID system were pyocyanin in Tech media; pyoverdin in Flo media; glucose fermentation, acid formation from glucose, maltose, lactose, sucrose, and mannitol in oxidation-fermentation media; Nitrate and nitrite reduction in nitrate

Original article PDF Seong Geun Hong1, Sunjoo Kim2, Seok Hoon Jeong3, Chulhun L. Chang4, Sung Ran Cho5, Ji Young Ahn5, Jong Hee Shin6, Hye Soo Lee7, Won Keun Song8, Young Uh9, Jong Hwa Yum10, Dongeun Yong10, Kyungwon Lee10, Yunsop Chong10 Department of Laboratory Medicine, Coll. of Med., Pochon CHA Univ.1, Gyeongsang Nat. Univ. School of Med.2, Coll. of Med. Kosin Univ.3, Pusan Nat. Univ. Coll. of Med.4, Sooncheonhyang Univ. Coll. of Med.5, Chonnam Nat. Univ. Med. School6, Chonbuk Univ. Med. School7, Hanllym Univ. Coll. of Med.8, Yonsei Univ. Wonju Coll. of Med.9, Yonsei Univ. Coll. of Med.10 Corresponding to Kyungwon Lee, E-mail: leekcp@yumc.yonsei.ac.kr Ann Clin Microbiol 2003;6(2):149-155.Copyright © Korean Society of Clinical Microbiology. Abstract Background：Increase in extended-spectrum β-lactamase(ESBL)-producing Escherichia coli and Klebsiella pneumoniae have been reported in Korea. The aim of this study was to determine the nationwide prevalence of ESBL-producing E. coli and K. pneumoniae, and to investigate the types of ESBLs. Methods：A total of 2,221 E. coli and

Original article PDF Seong Geun Hong1, Dongeun Yong2, Kyungwon Lee2, Eui-Chong Kim3, Wee Kyo Lee4, Seok Hoon Jeong5, Won Keun Song6, Yeon Jun Park7, Mi-Na Kim8, Young Uh9, Jong Hee Shin10, Jongwook Lee11, Ji Young Ahn12, Sun Wha Lee13, Jae Seok Kim5, Hee Bong Shin1 Departments of Laboratory Medicine, Coll. of Med., Pochon CHA Univ.1, Yonsei Univ. Coll. of Med.2 Seoul Nat. Univ. Coll. of Med.3, Ajou Univ. of Med.4, Coll. of Med. Kosin Univ.5, Hanllym Univ. Coll. of Med.6 The Catholic Univ. of Kor.7, Univ. of Ulsan Med. Coll. and Asan Med. Center8, Yonsei Univ. Wonju Coll. of Med.9 Chonnam Nat. Univ. Med. School10, Keonyang Univ. Med. Coll.11 Sooncheonhyang Univ. Coll. of Med.12; Neodin Med. Res. Center13 Corresponding to Kyungwon Lee, E-mail: leekcp@yumc.yonsei.ac.kr Ann Clin Microbiol 2003;6(1):29-36.Copyright © Korean Society of Clinical Microbiology. Abstract Background: A rapid increase of antimicrobial-resistant bacteria has become a serious problem in many countries. The aim of this

Case report PDF Young Uh1 , Soon Deok Park1, Gyu Yul Hwang1, Kap Jun Yoon1, Hwang Min Kim2, and Hyo Youl Kim3 Departments of Laboratory Medicine1, Pediatrics2 and Infectious Disease3, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Young Uh, E-mail: u931018@wonju.yonsei.ac.kr Ann Clin Microbiol 2004;7(2):186-189.Copyright © Korean Society of Clinical Microbiology. Abstract Verotoxin-producing Escherichia coli O157 is a primary cause of severe and bloody diarrhea. Campylobacter spp. are one of the commonly reported bacterial cause of gastrointestinal infections throughout the world. Only a few cases involving both E. coli O157 and Campylobacter species have been reported. The authors simultaneously isolated verotoxin-producing E. coli O157 and Campylobacter species from the stool of a 3 year-old male with bloody diarrhea, fever and abdominal pain. (Korean J Clin Microbiol 2004;7(2):186-189) Keywords Escherichia coli O157, Campylobacter, Gastroenteritis, Verotoxin

Original article PDF Seong Geun Hong1, Jongwook Lee2, Dongeun Yong3, Eui-Chong Kim4, Seok Hoon Jeong5, Yeon Jun Park6, Tae Yeal Choi7, Young Uh8, Jong Hee Shin9, Wee Kyo Lee10, Ji Young Ahn11, Sung-Hee Lee12, Gun-Jo Woo13, and Kyungwon Lee3 Departments of Laboratory Medicine, Coll. of Med., Pochon CHA Univ.1; Keonyang Univ. Med. Coll.2; Yonsei Univ. Coll. of Med.3; Seoul Nat. Univ. Coll. of Med.4; Coll. of Med. Kosin Univ.5; The Catholic Univ. of Kor.6; Hanyang Univ. Coll. of Med.7; Yonsei Univ. Wonju Coll. of Med.8; Chonnam Nat. Univ. Med. School9; Ajou Univ. Coll. of Med.10; Sooncheonhyang Univ. Coll. of Med.11; Cheju Hanmaeum Hospital12; Food Microbiology Division, Center for Food Safety Evaluation, Korea Food and Drug Administration13 Corresponding to Kyungwon Lee, E-mail: leekcp@yumc.yonsei.ac.kr Ann Clin Microbiol 2004;7(2):171-177.Copyright © Korean Society of Clinical Microbiology. Abstract Background : A rapid increase in antimicrobial-resistant bacteria has become a serious problem in Korea. Moreover, the

Original article PDF Jung Hyun Lee1, Il Kwon Bae2, Su Bong Kwon2, Seok Hoon Jeong2,Gun Jo Woo3, Jongwook Lee4, Wee Gyo Lee5, Jung Oak Kang6, Ji Young Ahn7, Seong Geun Hong8, Jong Hee Shin9, Young Uh10, Yeon Jun Park11, Eui-Chong Kim12, Kyungwon Lee13, Dongeun Yong13 Departments of Pediatrics1 and Laboratory Medicine2, Kosin University College of Medicine; Division of Food Microbiology, Korea Food and Drug Administration3; Departments of Laboratory Medicine, Keonyang University College of Medicine4, Ajou University College of Medicine5, Hanyang University College of Medicine6, Sooncheonhyang University College of Medicine7, Pochon CHA University College of Medicine8, Chonnam National University Medical school9 , Yonsei University Wonju College of Medicine10, The Catholic University of Korea College of Medicine11, Seoul National University College of Medicine12, Yonsei University College of Medicine13 Corresponding to Seok Hoon Jeong, E-mail: kscpjsh@ns.kosinmed.or.kr Ann Clin Microbiol 2004;7(2):111-118.Copyright © Korean Society of Clinical Microbiology. Abstract Background : The aims of this study were

Original article PDF Young Uh1, In Ho Jang1, Kap Jun Yoon1, and Hyo Youl Kim2 Departments of Laboratory Medicine1 and Infectious Disease2, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Young Uh, E-mail: u931018@yonsei.wonju.ac.kr Ann Clin Microbiol 2004;7(1):43-47.Copyright © Korean Society of Clinical Microbiology. Abstract Background : Group B streptococci (GBS) is known to be major cause of neonatal meningitis and bacteremia, and the infections also has been increasing in adults, particularly those with serious underlying diseases. This study is designed to define the trend of infection rate by year, underlying conditions, clinical outcome of group B streptococcal bacteremia, and to compare the distribution of age, sex, and serotype and antimicrobial susceptibility between invasive and noninvasive GBS infections. Methods : We analyzed the medical records of 36 cases with group B streptococcal bacteremia between 1990 and 2003, and compared the distribution of age, sex, serotype and antimicrobial susceptibility between

Case report PDF Young Uh1*, Byoung Geun Han2, Gue Yel Hwang1, Hyeun Gyeo Lee1, Kap Jun Yoon1, and Hyo Youl Kim2 Departments of Laboratory Medicine1, and Internal Medicine2, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Young Uh, E-mail: u931018@wonju.yonsei.ac.kr Ann Clin Microbiol 2005;8(1):90-93.Copyright © Korean Society of Clinical Microbiology. Abstract Listeria monocytogenes is the causative agent in a spectrum of human disease ranging from gastroenteritis to invasive infections such as meningitis, encephalitis, and septicemia. Elderly patients or persons who have lower cell-mediated immunity with predisposing conditions such as transplants, lymphomas, and AIDS, are especially susceptible. The tropism of L. monocytogenes for the central nervous system leads to severe disease, often with high mortality. We report a case of L. monocytogenes meningitis in a 58-year old woman with end stage renal disease. The patient was discharged without neurological sequelae after antibiotic treatment. (Korean J Clin Microbiol 2005;8(1):90-93) Keywords Meningitis, Listeria monocytogenes, End stage renal disease

Original article PDF Ji Hae Kang1, Il Kwon Bae2, Su Bong Kwon2, Seok Hoon Jeong2*, Jongwook Lee3, Wee Gyo Lee4, Jung Oak Kang5, Ji Young Ahn6, Seong Geun Hong7, Jong Hee Shin8, Young Uh9, Yeon Jun Park10, Eui-Chong Kim11, Kyungwon Lee12, Dongeun Yong12, and Gun Jo Woo13 Departments of Pediatrics1and Laboratory Medicine2, Kosin University College of Medicine, Busan; Department of Laboratory Medicine3, Keonyang University College of Medicine, Daejeon; Department of Laboratory Medicine4, Ajou University College of Medicine, Suwon; Department of Laboratory Medicine5, Hanyang University College of Medicine, Guri; Department of Laboratory Medicine6, Sooncheonhyang University College of Medicine, Gumi; Department of Laboratory Medicine7, Pochon Cha University College of Medicine, Sungnam; Department of Laboratory Medicine8, Chonnam National University Medical School, Gwangju; Department of Laboratory Medicine9, Yonsei University Wonju College of Medicine, Wonju; Department of Laboratory Medicine10, The Catholic University of Korea College of Medicine, Seoul; Department of Laboratory Medicine11, Seoul National University