Original article PDF Jung-Hee Lee, M.S.¹,², Byoung-Yoon Ahn, Ph.D.², Sung-Hwan Ban, M.D.³, Sang-Hyun Kim, M.D.³, and Eui-Chong Kim, M.D.¹,⁴ Virus Research Center, Clinical Research Institute, Seoul National University Hospital¹; Graduate School of Natural Resources, Korea University²; Department of Pediatrics, Kumi Hospital, Soonchunhyang University College of Medicine, Kumi³; Department of Clinical Pathology, Seoul National University College of Medicine⁴, Seoul, Korea Corresponding to Eui-Chong Kim, E-mail: euichong@plaza.snu.ac.kr Ann Clin Microbiol 2000;3(2):121-131.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The determination of serotype of enteroviruses is useful for the discrimination between sporadic and epidemic infections. The conventional serotyping method is time-consuming and labor-intensive. Recently, molecular method was introduced for the serotyping of enteroviruses. The aim of this study was to establish a method to isolate and analyze enteroviruses from various specimens utilizing molecular biological techniques and to determine which strains were phylogenetically related to clinical samples. Methods: Clinical samples in this study

Original article PDF Jongwook Lee, M.D., Nam Keum Lee*, M.T., Soo Hwan Pai*, M.D., Pum Soo Kim**, M.D., Won Choi, M.D., Don Hang Lee, M.D., Hyung Gil**, M.D., and Young Soo Kim**, M.D. Department of Clinical Pathology, Konyang University Hospital, Taejon, Departments of Clinical Pathology*, Internal Medicine**, Inha University Medical College, Inchon, Korea Corresponding to Jongwook Lee Ann Clin Microbiol 2000;3(2):111-115.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Helicobacter pylori (H. pylori) is closely associated with gastritis, peptic ulcer and gastric carcinoma. We evaluated the reliability and usefulness of ¹³C-urea breath test (¹³C-UBT) for the detection of H. pylori infection and searched for the cut-off value of the test. Method: We investigated 45 patients, who underwent esophagogastroduodenoscopy with multiple biopsy specimens taken for culture, histology and rapid urease test, and ¹³C-UBT. Sensitivity and specificity of UBT were calculated against the combined biopsy-based test results. Result: Of 45 patients, 26 were found to be H.

Original article PDF Soo Jin Choi, M.D., Sang Hyun Hwang, M.D., Joon Seok Park, M.D., Mi-Na Kim, M.D. and Chik Hyun Pai, M.D. Department of Clinical Pathology, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Korea Corresponding to Mi-Na Kim, E-mail: mnkim@amc.seoul.kr Ann Clin Microbiol 2000;3(1):69-74.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Although enriched broth cultures have been recommended as an adjuvant to the direct plating of tissue and body fluid specimens, the cost-effectiveness of broth cultures has been questioned in regard with the clinical significance of “broth only isolates (BOI)”. The purpose of this study was to investigate the usefulness of thioglycollate broth (THIO) cultures. Methods: We reviewed retrospectively results in the culture specimens of body fluids, tissue biopsies, and puses received during the month of July 1997. All specimens were inoculated into THIO in addition to agar plates. We reviewed the medical records

Case report PDF Hyun Lim, Dal Sik Kim, Hye Soo Lee, and Sam Im Choi Department of Laboratory Medicine, Chonbuk National University Medical School, and Institute for Medical Sciences, Chonju, Korea Corresponding to Hye Soo Lee, E-mail: leehs@moak.chonbuk.ac.kr Ann Clin Microbiol 2002;5(2):151-154.Copyright © Korean Society of Clinical Microbiology. Abstract Systemic infection due to Trichosporon beigelii is uncommon but increasingly reported in immunocompromised patients. Trichosporonosis is often refractory to conventional antifungal therapy and frequently fatal. We report a case of systemic T. beigelii infection in a patient with acute leukemia. The 35-year-old male patient had been diagnosed as acute myelogenous leukemia with severe neutropenia and received cytotoxic drug therapy. As a fever developed on the day 21 of chemotherapy, broad spectrum antibiotics were administered empirically. Even though an antifungal drug, amphotericin B was replaced because the blood cultures resulted in T. beigelii, the patient died of the septic shock. We think that T. beigelii should be included as a

Original article PDF Jung Hyun Lee1, Il Kwon Bae2, Su Bong Kwon2, Seok Hoon Jeong2,Gun Jo Woo3, Jongwook Lee4, Wee Gyo Lee5, Jung Oak Kang6, Ji Young Ahn7, Seong Geun Hong8, Jong Hee Shin9, Young Uh10, Yeon Jun Park11, Eui-Chong Kim12, Kyungwon Lee13, Dongeun Yong13 Departments of Pediatrics1 and Laboratory Medicine2, Kosin University College of Medicine; Division of Food Microbiology, Korea Food and Drug Administration3; Departments of Laboratory Medicine, Keonyang University College of Medicine4, Ajou University College of Medicine5, Hanyang University College of Medicine6, Sooncheonhyang University College of Medicine7, Pochon CHA University College of Medicine8, Chonnam National University Medical school9 , Yonsei University Wonju College of Medicine10, The Catholic University of Korea College of Medicine11, Seoul National University College of Medicine12, Yonsei University College of Medicine13 Corresponding to Seok Hoon Jeong, E-mail: kscpjsh@ns.kosinmed.or.kr Ann Clin Microbiol 2004;7(2):111-118.Copyright © Korean Society of Clinical Microbiology. Abstract Background : The aims of this study were

Case report PDF Bong Joon Oh1 , Jong Hee Shin1, Dong Hyun Shin2, Sook In Jeong2, Hyung Joon Kim2, Soon Pal Suh1, and Dong Wook Ryang1 Departments of Laboratory Medicine1 and Internal Medicine2, Chonnam National University Medical School, Gwangju, Korea Corresponding to Jong Hee Shin, E-mail: shinjh@chonnam.ac.kr Ann Clin Microbiol 2005;8(2):179-184.Copyright © Korean Society of Clinical Microbiology. Abstract Trichosporon beigelii is often resistant to the fungicidal effect of amphotericin B and can cause fatal disseminated infections in immunocompromised patients. We report a case of a disseminated T. beigelii infection with a favorable outcome in a patient with acute erythroleukemia and neutropenia. The patient presented a persistent fever, multiple erythematous skin lesions, and pulmonary infiltrates. T. beigelii was isolated from blood cultures in four days and also from cultures of abdominal skin lesion, sputum, and stool. The isolate was resistant to amphotericin B (MIC, 2 μg/mL), and the respective fluconazole and itraconazole MICs were 4 and 1 μg/mL. The patient

Case report PDF Eun-Mi Koh1,3, Chang Ki Kim1,3, Myungsook Kim3, Shin-Moo Kim4, Seung-Woo Park2, Hyun Cheol Chung2, Dongeun Yong1,3, Kyungwon Lee1,3, Yunsop Chong1,3 Departments of 1Laboratory Medicine and 2Internal Medicine, and 3Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul; 4Department of Clinical Laboratory Science, Wonkwang Health Science College, Iksan, Korea Corresponding to Kyungwon Lee, E-mail: leekcp@yumc.yonsei.ac.kr Ann Clin Microbiol 2007;10(2):164-167.Copyright © Korean Society of Clinical Microbiology. Abstract Vibrio fluvialis is a haplophilic gram-negative bacterium normally found in coastal water and seafood and causes gastroenteritis. There have been a few reports on V. fluvialis gastroenteritis in Korea, but no previous report of isolation from blood. We isolated V. fluvialis from the blood of two patients undergoing cancer chemotherapy. (Korean J Clin Microbiol 2007;10:164-167) Keywords Vibrio fluvialis, Blood culture, Bacteremia

Original article PDF Seong Deok Lee1, Hye Young Lee2, Hyun Chul Kim2, Soo Young Kim1 1Department of Laboratory Medicine, The Catholic University of Korea, St. Vincent’s Hospital, Suwon; 2 Department of Biomedical Life Science, the Graduate School of Health and Environment, Yonsei University, Seoul, Korea Corresponding to Seong Deok Lee, E-mail: start2033@paran.com Ann Clin Microbiol 2007;10(2):135-142.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The purpose of this study was to evaluate the usefulness of direct PCR for a rapid detection of Mycobacterium tuberculosis (MTB), the differentiation of MTB from nontuberculous mycobacteria (NTM), and the identification of NTM species in acid-fast bacilli (AFB) smear-positive specimens. Methods: A total of 255 AFB smear-positive respiratory specimens were studied. For the differentiation of MTB from NTM, the bands of the 360 bp, which exists both in MTB and NTM, and the 190 bp, which exists only in MTB, were amplified using the one- tube nested PCR targeting

Original article PDF Ji-Hyun Cho1,2,3, Hye-Soo Lee4, Key-Earn Lee1,2, Do-Sim Park1,2,3, Young-Jin Lee1,2,3, Hyung-Bae Moon2,3,5, Chang-Soo Choi2,3,6, Eun-Young Cho2,3,6, Haak-Cheoul Kim2,3,6 Departments of 1Laboratory Medicine, 5Pathology and 6Internal Medicine, 2Institute of Wonkwang Medical Science, 3Wonkwang Clinical Medicine Research Center, Wonkwang University College of Medicine, Iksan; 4Department of Laboratory Medicine, Chonbuk National University College of Medicine, Jeonju, Korea Corresponding to Haak-Cheoul Kim, E-mail: kimpb@wonkwang.ac.kr Ann Clin Microbiol 2007;10(1):25-31.Copyright © Korean Society of Clinical Microbiology. Abstract Background: As oral antiviral treatment for chronic hepatitis B increases, quantitation of viral load has become an essential test for HBV management, and assays using real-time PCR principles have been introduced recently. Methods: We analysed the analytical performance (precision, linear range, and sensitivity) of RealArt HBV LC PCR Reagents (Artus GmbH, Hamburg, Germany), its correlation with COBAS AMPLICOR HBV MONITOR Test (Roche Diagnostics, Mannheim, Germany), and distribution of viral load in the patients’ sera according to antiviral treatment and presence of HBeAg.

Original article PDF Ja Young Lee1, Jeong Hwan Shin1,2, Hyun-Kyung Lee3, Seong-Mi Yu5, Eun Hee Park6, Hee Ryune Lee1, Jae Hyen Kim1, Hye Ran Kim1, Chi Sook Moon3, Young Jae Kim4, Jeong Nyeo Lee1,2 1Department of Laboratory Medicine, 2Paik Institute for Clinical Research, Departments of 3Internal Medicine and 4Anesthesiology, College of Medicine, Inje University, Busan; 5Department of Nursing, Gwangju Health College, Gwangju; 6Busan Metropolitan City Institute of Health and Environment, Busan, Korea Corresponding to Jeong Hwan Shin, E-mail: jhsmile@inje.ac.kr Ann Clin Microbiol 2008;11(1):29-34.Copyright © Korean Society of Clinical Microbiology. Abstract Background: We noticed a sudden increase in the isolation of Klebsiella oxytoca from bronchial washing specimens during May to June 2006. An epidemiological investigation was conducted to identify the cause of the outbreak and to implement appropriate infection control measures.  Methods: A total of 18 isolates of K. oxytoca were found. The 14 bronchial washing specimens that yielded K. oxytoca were taken in the outpatient bronchoscopy suite, and the other 4