Case report PDF Jong Hyun Kim1, Eun Gyoung Lim2, Hyun Chul Jang1, Ju Young Park2, Sun Jin Lee1, Mi Sun Park1, Gil Bae Choi2, Bok Kwon Lee1 1Division of Enteric Bacterial Infections, Center for Infectious Diseases, National Institute of Health, Seoul, 2Microbe Division, Ulsan Institute of Health and Environment, Ulsan, Korea Corresponding to Bok Kwon Lee, E-mail: bokrates@nih.go.kr Ann Clin Microbiol 2009;12(1):48-52.Copyright © Korean Society of Clinical Microbiology. Abstract Bacillus cereus causes two types of gastrointestinal diseases: emesis and diarrhea. It produces one emetic toxin and nine different enterotoxins. In March 2008, eight of a family became sick after eating slices of raw fish. We isolated emetic toxin producing B. cereus from the stools of 6 patients and 2 subclincal humans. In this study, the presence of enterotoxin genes, such as those of haemolysin BL (Hbl), nonhemolytic enterotoxin (Nhe), B. cereus enterotoxin T (BceT), enterotoxin FM (EntFM), cytotoxin K (cytK) and cereulide were assayed by polymerase

Original article PDF Soo Jin Yoo1, Choong-Hee Noh2,3, Hyeon Mi Yoo2, Won Chang Shin4, Soo Jeon Choi4, Baek-Nam Kim4, Chang Keun Kim5, Myoung-Jae Chey5, Kyunam Kim6, Sang-Lae Lee7, Eun-Young Kuak1, Bo-Moon Shin1,2 Departments of 1Laboratory Medicine, 2Infection Control Office, 3Urology, 4Internal Medicine, 5Pediatrics, 6Family Medicine, and 7Emergency Medicine, Sanggye Paik Hospital, Inje University College of Medicine, Seoul, Korea Corresponding to Bo-Moon Shin, E-mail: bmshin@unitel.co.kr Ann Clin Microbiol 2010;13(3):103-108. https://doi.org/10.5145/KJCM.2010.13.3.103Copyright © Korean Society of Clinical Microbiology. Abstract Background: The aim of this study is to clarify the epidemiology of swine-origin influenza A (H1N1) virus 2009 (S-OIV) during the first month of outbreak at one of influenza clinic in Seoul, Korea.  Methods: We documented the epidemiologic and clinical features of S-OIV-confirmed cases who visited a university hospital in Northeastern Seoul between August 21 and September 20, 2009. Nasopharyngeal swab of patients with acute febrile respiratory illnesses were evaluated with rapid influenza antigen

Original article PDF Hye-young Wang1,*, Hyunwoo Jin2,*, Hyeeun Bang2, Yeon-Im Choi2, Eun-mi Park3, Won-jung Koh4, Hyeyoung Lee2 1M&D, Inc. 2Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, 3YD Diagnostics, Yongin, 4Division of Pulmonary and Critical Care Medicine, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea Corresponding to Hyeyoung Lee, E-mail: hyelee@yonsei.ac.kr Ann Clin Microbiol 2011;14(3):103-109. https://doi.org/10.5145/KJCM.2011.14.3.103Copyright © Korean Society of Clinical Microbiology. Abstract Background: The differentiation of Mycobacterium tuberculosis (MTB) from nontuberculous mycobacteria (NTM) is of primary importance for infection control and choice of antimicrobial therapy. The diagnosis of diseases caused by NTM is difficult because NTM are prevalent in the environment and have fastidious properties. In this study, we evaluated the real-time PCR-based MTB/NTM detection kit for its usefulness in discrimination of MTB and NTM species. Methods: A total of 155 sputum specimens whose AFB staining smear and culture were

Review article PDF Mi Hee Jang1, Go Eun Choi1, Chulhun L. Chang1, Yeong Dae Kim2 Departments of 1Laboratory Medicine, and 2Thoracic and Cardiovascular Surgery, School of Medicine, Pusan National University, Busan, Korea Corresponding to Yeong Dae Kim, E-mail: domini@pnu.edu Ann Clin Microbiol 2011;14(2):41-47. https://doi.org/10.5145/KJCM.2011.14.2.41Copyright © Korean Society of Clinical Microbiology. Abstract Molecular strain typing of Mycobacterium tuberculosis is important for the detection of outbreaks of tuberculosis and laboratory cross contamination, as well as the differentiation between re-infection and reactivation of tuberculosis. In the present review, the authors investigated the currently available typing methods for M. tuberculosis and the current status of strain distribution in Korea. IS6110-restriction fragment length polymorphism (RFLP), which is considered a standard method, is based on numbers and positions of the insertion sequence, IS6110. The method has an excellent discriminatory power with a considerable amount of worldwide data, although it is time-consuming and labor-intensive. Spoligotyping is based

Original article PDF Dong-Hyun Lee1, Eun-Ha Koh1, Sae-Rom Choi1, Sunjoo Kim1, Dong Hoon Kim2, Nam Yong Lee3 Departments of 1Laboratory Medicine, 2Emergency Medicine, Gyeongsang Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju, 3Department of Laboratory Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea Corresponding to Sunjoo Kim, E-mail: sjkim8239@hanmail.net Ann Clin Microbiol 2013;16(4):168-173. https://doi.org/10.5145/ACM.2013.16.4.168Copyright © Korean Society of Clinical Microbiology. Abstract Background: This study compared the growth of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Streptococcus pneumoniae, and Haemophilus influenzae in blood culture bottles containing anticoagulants, sodium polyanethol sulfonate (SPS) and sodium citrate. Methods: One hundred and fifty colony forming units of five different bacterial species were inoculated into standard aerobic (SA) and standard anaerobic (SN) bottles and were combined with 5 mL of human blood in solution with SPS or sodium citrate. Time to detection (TTD) was then monitored using the BacT/ Alert 3D

Case report PDF Kwang-Sook Woo, Jae-Lim Choi, Bo-Ram Kim, Ji-Eun Kim, Kyeong-Hee Kim, Jeong-Man Kim, Jin-Yeong Han Department of Laboratory Medicine, Dong-A University College of Medicine, Busan, Korea Corresponding to Jin-Yeong Han, E-mail: jyhan@dau.ac.kr Ann Clin Microbiol 2014;17(1):20-22. https://doi.org/10.5145/ACM.2014.17.1.20Copyright © Korean Society of Clinical Microbiology. Abstract Shigella bacteremia is rare, occurring mainly in children. Shigella species often cause diarrhea or gastrointestinal inflammation in humans and are rarely associated with bacteremia. This report describes an unusual case of Shigella boydii bacteremia in an 84-year-old patient visiting our hospital after experiencing nausea, vomiting, and febrile sensation for 2 days. Peripheral blood cultures revealed S. boydii and 16S rDNA sequence analysis produced the same result. However, the organism was not isolated from the patient’s stool. She was started on ciprofloxacin, to which this organism is sensitive, and was subsequently discharged with instructions to complete a 14-day course of ciprofloxacin. Shigellosis is usually a

Original article PDF Hae In Bang1, Hyun Mi Lim1, Eui Young Jang1, Eun Su Park1, Eun Jung Lee2, Tae Hyong Kim2, Rojin Park1, Jeong Won Shin1, Tae Youn Choi1 1Department of Laboratory Medicine, 2Division of Infectious Diseases, Department of Internal Medicine, Soonchunhyang University Hospital, Seoul, Korea Corresponding to Tae Youn Choi, E-mail: choity@schmc.ac.kr Ann Clin Microbiol 2015;18(3):88-93. https://doi.org/10.5145/ACM.2015.18.3.88Copyright © Korean Society of Clinical Microbiology. Abstract Background: Blood culture is a critical test for diagnosing bloodstream infections. Frequent microbial contamination during sampling and testing leads to abuse of antimicrobial agents. We evaluated methods for reducing contamination and obtaining more reliable results. Methods: We analyzed blood cultures obtained between 2009 and 2015. We established 6 quality indicators: true positive rate, contamination rate, blood sampling volume, number of sets of blood cultures, delayed transportation rate, and percentage of samples collected from the femoral region, with reference to the CLSI guideline M47-A, 2007. Education