Case report PDF Yangsoon Lee, Eun-Mi Koh, Myungsook Kim, Dongeun Yong, Seok Hoon Jeong, Kyungwon Lee, Yunsop Chong Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea Corresponding to Kyungwon Lee, E-mail: leekcp@yuhs.ac Ann Clin Microbiol 2010;13(3):125-127. https://doi.org/10.5145/KJCM.2010.13.3.125Copyright © Korean Society of Clinical Microbiology. Abstract Clostridium citroniae is a novel species reclassified from C. clostridioforme. Clostridium species are obligate anaerobes and spore-forming gram-positive rods. However, C. citroniae stains gram negative and does not consistently produce spores, making it difficult to identify. We isolated C. citroniae from the blood and peritoneal fluid of one patient, and from the blood of another patient, both of whom were undergoing cancer chemotherapy. (Korean J Clin Microbiol 2010;13:125-127) Keywords Clostridium citroniae, Bacteremia

Original article PDF Younhee Park1, Hee Bong Shin2,7, Chang Ki Kim3,7, Kyoung Ho Roh4,7, Jong Hwa Yum5,7, Dongeun Yong6,7, Seok Hoon Jeong6,7, Kyungwon Lee6,7 Department of Laboratory Medicine, 1Kwandong University College of Medicine, Goyang, 2Soonchunhyang University College of Medicine, 4Korea University College of Medicine, Seoul, 5Department of Clinical Laboratory Science, Dongeui University, Busan, 6Department of Laboratory Medicine and 7Research Institute of Bacterial Resistance, Yonsei University College of Medicine, 3Korean Institute of Tuberculosis, Seoul, Korea Corresponding to Dongeun Yong, E-mail: deyong@yuhs.ac Ann Clin Microbiol 2010;13(1):34-39. https://doi.org/10.5145/KJCM.2010.13.1.34Copyright © Korean Society of Clinical Microbiology. Abstract Background: Accurate and rapid identification of pathogens is one of the most important tasks of the clinical microbiology laboratory, and, in cases of rare pathogens, the identification is difficult and time-consuming upon the use of conventional methods alone. Herein, we will report our molecular work involving the identification of bacteria and fungi.  Methods: Sixty bacterial isolates had been

Original article PDF Kyoung Ho Roh1, Chang-Ki Kim2, Jong Hwa Yum3, Dongeun Yong4,5, Seok Hoon Jeong4,5, Chae Seung Lim1, Chang Kyu Lee1, Yunjung Cho1, Kyungwon Lee4,5, Yunsop Chong4,5 1Department of Laboratory Medicine, Korea University College of Medicine, 2Korean Institute of Tuberculosis, Seoul, 3Department of Clinical Laboratory Science, Dongeui University, Busan, 4Department of Laboratory Medicine and 5Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea Corresponding to Kyungwon Lee, E-mail: leekcp@yuhs.ac Ann Clin Microbiol 2010;13(1):27-33. https://doi.org/10.5145/KJCM.2010.13.1.27Copyright © Korean Society of Clinical Microbiology. Abstract Background: Increasing numbers of Acinetobacter spp. resistant to multiple drugs, including carbapenem, has been a serious problem. The aims of this study were to determine carbapenem resistance patterns and mechanisms, as well as to study the molecular epidemiology of Acinetobacter spp. Methods: Clinical isolates of Acinetobacter spp. were collected from May to November in 2006. Antimicrobial susceptibility testing was performed using CLSI disk diffusion and

Original article PDF Hyukmin Lee1, Eun-Mi Koh2, Chang Ki Kim3, Jong Hwa Yum4, Kyungwon Lee5, Yunsop Chong5 1Department of Laboratory Medicine, Kwandong University College of Medicine, Goyang, 2 Incheon Blood Center, Korean Red Cross, Incheon, 3Korean Institute of Tuberculosis, Seoul, 4Department of Clinical Laboratory Science, Dongeui University, Busan, 5Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea Corresponding to Kyungwon Lee, E-mail: leekcp@yuhs.ac Ann Clin Microbiol 2010;13(1):19-26. https://doi.org/10.5145/KJCM.2010.13.1.19Copyright © Korean Society of Clinical Microbiology. Abstract Background: Blood culture is essential for diagnosis of sepsis. However, usually the available blood volume is not sufficient to meet the guidelines. Thus, periodic monitoring and feedback are essential to iBackground: Recently a novel plasmid-mediated resistant mechanism that conferred high-level resistance to aminoglycoside via methylation of 16S rRNA was reported. The aims of this study were to determine the prevalence of the 16S rRNA methylase genes and

Case report PDF Seri Jeong1, Ji Yeon Park1, Sang Hoon Han2, Yangsoon Lee1,3, Dongeun Yong1, Kyungwon Lee1, Yunsop Chong1 1Department of Laboratory Medicine and Research Institute of Bacterial Resistance, 2Department of Internal Medicine, Yonsei University College of Medicine, Seoul, 3Department of Laboratory Medicine, National Health Insurance Corporation Ilsan Hospital, Goyang, Korea Corresponding to Dongeun Yong, E-mail: deyong@yuhs.ac Ann Clin Microbiol 2011;14(4):144-147. https://doi.org/10.5145/KJCM.2011.14.4.144Copyright © Korean Society of Clinical Microbiology. Abstract A 60-year-old man presented with a 1-day history of fever, vomiting, and diarrhea. He was diagnosed with severe septic shock on the basis of a body temperature of 38.9℃, heart rate of 92/min, respiratory rate of 25/min, WBC count of 22,970/μL, C-reactive protein (CRP) level of 136 mg/L, blood urea nitrogen (BUN) of 34.0 mg/dL, and creatinine of 2.98 mg/dL. On blood culture, Gram-positive cocci were detected in all 6 bottles. Small grayish non-hemolytic colonies were found on blood agar plates

Case report PDF Yong Hyun Kim1, Eun-Mi Koh2, Yong-Wha Lee1, You Kyoung Lee1, Hee Bong Shin1, Kyungwon Lee2 1Department of Laboratory Medicine and Genetics, Soonchunhyang University College of Medicine, Bucheon, 2Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea Corresponding to Hee Bong Shin, E-mail: shinhb@schmc.ac.kr Ann Clin Microbiol 2011;14(3):115-117. https://doi.org/10.5145/KJCM.2011.14.3.115Copyright © Korean Society of Clinical Microbiology. Abstract Brevundimonas diminuta is a lactose non-fermenting Gram-negative rod associated with infection in immunocompromised patients. In three patients from two general wards, B. diminuta was isolated in blood culture sample. The clinical features of the patients did not coincide with the blood culture result, and pseudo-outbreak was suspected. These isolated were biochemically identified as Brevundimonas diminuta, and 16S rRNA sequencing confirmed their identification. The PFGE result showed a single pattern, and their clonality was assumed. (Korean J Clin Microbiol 2011;14:115-117) Keywords Brevundimonas diminuta, Pseudo-outbreak, Pulsed-field

Original article PDF Ji-Sook Yim, Seock-Mi Hwang, Myungsook Kim, Hee-Joung Lim, Saeam Shin, Hae-Sun Chung, Heejung Kim, Kyungwon Lee Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea Corresponding to Heejung Kim, E-mail: hjkim12@yuhs.ac Ann Clin Microbiol 2012;15(3):88-91. https://doi.org/10.5145/KJCM.2012.15.3.88Copyright © Korean Society of Clinical Microbiology. Abstract Background: Clostridium difficile is the main etiologic agent of antibiotic-associated diarrhea and the most common cause of hospital-acquired diarrhea. Recently, the incidence of C. difficile infections (CDI) has increased and new highly virulent C. difficile strains have emerged. Therefore, accurate and rapid diagnosis is needed. We compared the results of using chromID C. difficile (chromID CD, bioMeriéux, France) with the conventional C. difficile Selective Agar (CDSA; BD, USA) for the isolation of C. difficile. Methods: A total of 738 stool specimens of suspected CDI patients at the Severance Hospital from July to August 2011 were inoculated

Original article PDF Myungsook Kim, Mi Jung Kwon, Hae-Sun Chung, Yangsoon Lee, Dongeun Yong, Seok Hoon Jeong, Kyungwon Lee, Yunsop Chong Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea Corresponding to Dongeun Yong, E-mail: deyong@yuhs.ac Ann Clin Microbiol 2012;15(2):60-66. https://doi.org/10.5145/KJCM.2012.15.2.60Copyright © Korean Society of Clinical Microbiology. Abstract Background: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been used for the identification of bacteria worldwide. To our knowledge, the evaluation of MALDI-TOF MS for the identification of bacteria in Korea has not been studied. In this paper we compared the identification results of aerobic bacteria using MALDI-TOF MS to those results using conventional biochemical methods. Methods: We evaluated the performance of a MALDI- TOF MS system (Bruker Daltonics, Leipzig, Germany) on consecutive aerobic isolates collected from January to February of 2011 which were identified using conventional methods (biochemical testing

Original article PDF Hae-Sun Chung1, Hyukmin Lee2, Yangsoon Lee1, Dongeun Yong1, Seok Hoon Jeong1, Bok-Kwon Lee3, Suk-Chan Jung4, Suk-Kyung Lim4, Kyungwon Le1, Yunsop Chong1 1Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 2Department of Laboratory Medicine, Kwandong University College of Medicine, Goyang, 3Division of Enteric Bacterial Infections, Center for Infectious Diseases, Korea National Institute of Health, Cheongwon, 4Animal, Plant and Fisheries Quartine and Inspection Agency, Anyang, Korea Corresponding to Kyungwon Lee, E-mail: leekcp@yuhs.ac Ann Clin Microbiol 2012;15(1):14-20. https://doi.org/10.5145/KJCM.2012.15.1.14Copyright © Korean Society of Clinical Microbiology. Abstract Background: The emergence of non-typhoidal Salmonella (NTS) with decreased susceptibilities to fluoroquinolone, ampicillin, or ceftriaxone has been reported worldwide. However, current surveillance studies of resistance among NTS in Korea are limited. Thus, the antimicrobial susceptibilities; resistance mechanisms such as extended-spectrum β-lactamase (ESBL), plasmid-mediated AmpC β-lactamase (PABL), and plasmid-mediated quinolone resistance (PMQR); and molecular epidemiologic characteristics were investigated