Original article PDF Young Uh, In Ho Jang, Gyu Yel Hwang, Kap Jun Yoon and Hyung-Hoan Lee* Department of Clinical Pathology, Yonsei University Wonju College of Medicine, Wonju, Korea, and Department of Biology, Kon-Kuk University, Seoul, Korea* Corresponding to Young Uh, E-mail: u931018@wonju.yonsei.ac.kr Ann Clin Microbiol 1999;2(1):58-63.Copyright © Korean Society of Clinical Microbiology. Abstract Background：The accurate and rapid identification of enterococci can provide clinician’s decision making of antimicrobial therapy because enterococci are usually multiresistant to commonly used antimicrobial agents and antimicrobial resistance patterns are different according to enterococcal species. Accuracy of identification system depends mainly on data base such as positive rate of biochemical reactions, relative frequency of occurrence of biotype, and isolation frequency of microorganisms. The purpose of this study was to analyze the isolation rate and biotype frequency of enterococci isolated from clinical specimens. Methods：We used a simplified identification system for the identification of the enterococci from clinical

Review article PDF Kyungwon Lee, M.D. and Yunsop Chong, Ph.D. Department of Clinical Pathology and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea Corresponding to Kyungwon Lee Ann Clin Microbiol 2000;3(2):83-88.Copyright © Korean Society of Clinical Microbiology. Abstract 감염 질환을 흔히 일으키는 세균 중에 항균제 내성의 혼합을 이미 많이 알려져 있다. 특히 트랜스포존이 세 널리 사용되는 항균제에 대한 다제 내성 세균이 대단히 흔해졌다. Pseudomonas 감염 치료를 위해서는 유효한 항균제 선택에 어려움을 겪는 경우가 흔하다(1). 그람음성 간균 내성 유전자는 plasmid, 염색체 혹은 transposon에 들어있는 것이 발견되어, 이들 중에는 여러 가지 내성 유전자를 가지고 있어 다제 내성을 나타낸다. 내성 유전자가 integron이라고 하는 구조 중에 들어 있는 경우가 있다. 즉, 세균의 염색체나 plasmid 혹은 transposon이 있고, plasmid나 transposon 구조 중에는 integron이 있고, integron 중에는 cassette가 있으며, cassette 중에는 내성 유전자가 들어있는 경우가 있다(2). Integron은 현재 4 종류가 알려져 있으며, 그 중 class 1, 2 및 3 integron은 항균제 내성

Original article PDF Young Uh, M.D., In Ho Jang, M.S., Kap Jun Yoon, M.D., Hyun Soo Kim*, M.D., and Hyo Youl Kim*, M.D. Departments of Clinical Pathology and Internal Medicine*, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Young Uh Ann Clin Microbiol 2001;4(1):28-32.Copyright © Korean Society of Clinical Microbiology. Abstract Background : Vibrio species may be classified as halophilic or nonhalophilic on the basis of their requirement of NaCl for optimal growth. Recently, attention has been focused on the halophilic vibrios and Vibrio cholerae non-O1/O139 causing extraintestinal infections such as septicemia. The aim of this study is to elucidate the isolation rate and clinical manifestations of Vibrio species isolated from clinical specimens between 1996 and 2000 at Wonju Christian Hospital. Methods : Stool specimens were inoculated onto the thiosulfate-citrate-bile salt-sucrose media, blood cultures were performed by automated blood culture systems with commercial bottles, and the others were cultured according to the routine procedures. Results :

Case report PDF Chong-Rae Cho, Tae-Hyun Um, and Jae-Won Jeong1 Departments of Laboratory Medicine and Internal Medicine1, College of Medicine, Inje University, Ilsanpaik Hospital Goyang, Korea Corresponding to Chong Rae Cho, E-mail: chocr@ilsanpaik.ac.kr Ann Clin Microbiol 2005;8(2):185-188.Copyright © Korean Society of Clinical Microbiology. Abstract There are two stages in the life circle of Plasmodium spp in humans: exoerythrocytic and erythrocytic stages. Hydroxychloroquine is the major chemotherapeutic agent against malarial parasites in their erythrocytic stage. The recurrence of Plasmodium vivax malaria, which is usually caused by an inadequate treatment or the presence of drug resistant parasites, has been reported frequently in the world, but rarely in Korea. We experienced two patients who recurred with P. vivax malaria after hydroxychloroquine therapy, and treating with insufficient doses of the drug was suspected as the cause of the recurrence. (Korean J Clin Microbiol 2005;8(2):185-188) Keywords Plasmodium vivax, Inadequate treatment, Recurrence

Original article PDF Chong Rae Cho, Tae Hyun Um, and In Ki Paik1 Department of Laboratory Medicine, College of Medicine, Inje University, Ilsanpaik Hospital, and Seoul Seobu Blood Center, Korea Red Cross1, Seoul, Korea Corresponding to Chong Rae Cho, E-mail: chocr@ilsanpaik.ac.kr Ann Clin Microbiol 2005;8(2):136-141.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Yersinia pseudotuberculosis is recognized throughout the world as a cause of water- or food born infections in human and animals. Although many attempts have been made to define optimal conditions for the isolation of the organism from water, their isolation yields remain low; therefore, we tried to find an effective method for the recovery of Y. pseudotuberculosis from water. Methods: Water samples were deliberately contaminated with Y. pseudotuberculosis at various levels and then processed by the following three isolation methods: centrifugation, direct filtration, and intracellular culture. For the centrifugation method, the water samples were centrifuged at 5000 rpm for 1 hr and the

Case report PDF Gyoung Yim Ha, and Moon Yeun Kim* Department of Laboratory Medicine, College of Medicine, Dongguk University, Kyongju, Korea Corresponding to Moon Yeun Kim, E-mail: kmy@dongguk.ac.kr Ann Clin Microbiol 2005;8(1):99-104.Copyright © Korean Society of Clinical Microbiology. Abstract The introduction of a new, fully automated system into the clinical microbiology laboratory contributes to a rapid identification of microorganisms with accurate and reliable results, but such a system requires a high cost and additional tests for identification of some species. For instance, additional tests on oxidase, indole, motility, hemolysis, and pigmentation are needed in the correct identification by using Vitek GNI+ system (bioMerieux Vitek Inc., MO, USA). In particular, Vibrio and Aeromonas species are occasionally identified incorrectly when an automated system is used, and thus conventional biochemical tests may be more reliable in the identification of such species. We experienced three cases of incorrect identification of Vibrio parahaemolyticus, Vibrio cholerae, and Aeromonas veronii biovar sobria as Vibrio alginolyticus by using

Original article PDF Mi-Kyung Lee, Bo-Rae G. Pa Department of Laboratory Medicine, Chung-Ang University College of Medicine, Seoul, Korea Corresponding to Mi-Kyung Lee, E-mail: cpworld@cau.ac.kr Ann Clin Microbiol 2007;10(2):150-153.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Colonial morphology of Candida albicans known as ‘spiking’ on a primary isolation blood agar plate (BAP) allows rapid and presumptive identification of C. albicans. We evaluated the ‘spiking’ appearance to identify C. albicans. Methods: A total of 144 fully identified clinical isolates of yeasts and 10 type strains of yeasts were tested. All isolates obtained from the 5% CO2 incubation on BAP and chocolate agar plate (CHOC) were macroscopically examined for the presence of an irregular margin (spiking). The germ tube test was performed by incubating test organisms in 0.5 mL of pooled human sera. Results: The sensitivity for BAP-spiking, CHOC-spiking and germ tube test were 93.7%, 91.1%, and 98.7%, respectively. The specificity for three methods was