Original article PDF Wonkeun Song1, Jae-Seok Kim1, Han-Sung Kim1, Tae Jae Lee1, Min-Jeong Park1, In-Heon Park2, and Kyu Man Lee1 Department of Laboratory Medicine1and Orthopedic Surgery2, Hallym University College of Medicine, Seoul, Korea Corresponding to Wonkeun Song, E-mail: swonkeun@hallym.or.kr Ann Clin Microbiol 2004;7(2):124-129.Copyright © Korean Society of Clinical Microbiology. Abstract Background : Klebsiella oxytoca strain exhibiting an unusual inducible β-lactam resistance phenotype was isolated from a wound specimen of a patient at a university hospital in August 2002. The isolate was resistant to ampicillin, ampicillin-sulbactam, cephalothin, cefoxitin, and demonstrated reduced inhibition zone diameters for ceftazidime in combination with clavulanate versus those for ceftazidime when tested alone. Methods : Antimicrobial susceptibilities were tested using the Etest and disk diffusion method. AmpC β-lactamase production was determined by modified Hodge test. The disk antagonism method was used to detect inducibility of β-lactamase. Conjugation experiments were performed by the filter mating method using the recipient Escherichia coli J53 Azir strain. PCR and

Original article PDF Jung Hyun Lee1, Il Kwon Bae2, Su Bong Kwon2, Seok Hoon Jeong2,Gun Jo Woo3, Jongwook Lee4, Wee Gyo Lee5, Jung Oak Kang6, Ji Young Ahn7, Seong Geun Hong8, Jong Hee Shin9, Young Uh10, Yeon Jun Park11, Eui-Chong Kim12, Kyungwon Lee13, Dongeun Yong13 Departments of Pediatrics1 and Laboratory Medicine2, Kosin University College of Medicine; Division of Food Microbiology, Korea Food and Drug Administration3; Departments of Laboratory Medicine, Keonyang University College of Medicine4, Ajou University College of Medicine5, Hanyang University College of Medicine6, Sooncheonhyang University College of Medicine7, Pochon CHA University College of Medicine8, Chonnam National University Medical school9 , Yonsei University Wonju College of Medicine10, The Catholic University of Korea College of Medicine11, Seoul National University College of Medicine12, Yonsei University College of Medicine13 Corresponding to Seok Hoon Jeong, E-mail: kscpjsh@ns.kosinmed.or.kr Ann Clin Microbiol 2004;7(2):111-118.Copyright © Korean Society of Clinical Microbiology. Abstract Background : The aims of this study were

Original article PDF Wonkeun Song*, Tae Jae Lee, Taek-Kyung Kim, Han-Sung Kim, Jae-Seok Kim, Min-Jeong Park, and Kyu Man Lee Department of Laboratory Medicine, Hallym University College of Medicine, Seoul, Korea Corresponding to Wonkeun Song, E-mail: swonkeun@hallym.or.kr Ann Clin Microbiol 2005;8(2):160-164.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The aim of this study was to evaluate the Vitek system and the disk diffusion method for susceptibility testing of Enterobacteriaceae against piperacillin-tazobactam (TZP) and methicillin-resistant Staphylococcus aureus(MRSA) against trimethoprim-sulfamethoxazole (SXT) using the broth microdilution method as the reference. Methods: Using the Vitek system and the disk diffusion method, we tested 96 isolates of Enterobacteriaceae (48 Escherichia coli, 26 Klebsiella pneumoniae, 8 Serratia marcescens, 6 Enterobacter cloacae, 2 E. aerogenes, 2 K. oxytoca, 2 Citrobacter freundii, 2 Pantoea agglomerans) and 61 isolates of MRSA for susceptibity against TZP and SXT, respectively; the broth microdilution of National Committee for Clinical Laboratory Standards was used as the reference method. Results: In the susceptibility testing of Enterobacteriaceae against TZP, Vitek system yielded

Original article PDF Ji Hae Kang1, Il Kwon Bae2, Su Bong Kwon2, Seok Hoon Jeong2*, Jongwook Lee3, Wee Gyo Lee4, Jung Oak Kang5, Ji Young Ahn6, Seong Geun Hong7, Jong Hee Shin8, Young Uh9, Yeon Jun Park10, Eui-Chong Kim11, Kyungwon Lee12, Dongeun Yong12, and Gun Jo Woo13 Departments of Pediatrics1and Laboratory Medicine2, Kosin University College of Medicine, Busan; Department of Laboratory Medicine3, Keonyang University College of Medicine, Daejeon; Department of Laboratory Medicine4, Ajou University College of Medicine, Suwon; Department of Laboratory Medicine5, Hanyang University College of Medicine, Guri; Department of Laboratory Medicine6, Sooncheonhyang University College of Medicine, Gumi; Department of Laboratory Medicine7, Pochon Cha University College of Medicine, Sungnam; Department of Laboratory Medicine8, Chonnam National University Medical School, Gwangju; Department of Laboratory Medicine9, Yonsei University Wonju College of Medicine, Wonju; Department of Laboratory Medicine10, The Catholic University of Korea College of Medicine, Seoul; Department of Laboratory Medicine11, Seoul National University

Original article PDF Il Kwon Bae1, Yu Nae Lee1, Seok Hoon Jeong1, Kyungwon Lee2, Dongeun Yong2, Jongwook Lee3, Seong Geun Hong4, Eui-Chong Kim5, Yeon Jun Park6, Tae Yeal Choi7, Young Uh8, Jong Hee Shin9, Wee Gyo Lee10, Ji young Ahn11, Sung-Hee Lee12, Gun-Jo Woo13, Hyo-Sun Kwak13 Department of Laboratory Medicine1, Kosin University College of Medicine, Busan; Department of Laboratory Medicine2, Yonsei University College of Medicine, Seoul; Department of Laboratory Medicine3, Keonyang University College of Medicine, Daejeon; Department of Laboratory Medicine4, Pochon Cha University College of Medicine, Sungnam; Department of Laboratory Medicine5, Seoul National University College of Medicine; Department of Laboratory Medicine6, The Catholic University College of Medicine; Department of Laboratory Medicine7, Hanyang University College of Medicine, Seoul; Department of Laboratory Medicine8, Yonsei University Wonju College of Medicine9, Wonju; Department of Laboratory , Chonnam National University College of Medicine, Gwangju; Department of Laboratory Medicine10, Ajou University College of Medicine, Suwon; Department

Original article PDF Sun Min Lee1 , Eun Joo Song1, Eun Kyoung Yang1, Il Kwon Bae2, Seok Hoon Jeong2, Jeong Man Kim3, Eun Yup Lee1, Chulhun L. Chang1 Department of Laboratory Medicine1, Pusan National University College of Medicine; Department of Laboratory Medicine2, Kosin University College of Medicine; and Department of Laboratory Medicine3, Dong-A University College of Medicine, Busan, Korea Corresponding to Chulhun L. Chang, E-mail: cchl@pusan.ac.kr Ann Clin Microbiol 2006;9(1):13-17.Copyright © Korean Society of Clinical Microbiology. Abstract Background: As clinical isolates of Staphylococcus aureus with reduced inhibition zone of arbekacin in disk diffusion susceptibility tests are observed frequently, we examined their susceptibility to the antibiotic by comparing the results of the agar dilution testing with those of disk diffusion testing. Methods: During the period of May through July, 2004, 88 isolates of methicillin-resistant and 11 methicillin-susceptible S. aureus were collected from clinical specimens in Pusan National University Hospital and Kosin University Gospel Hospital. Minimal inhibitory

Original article PDF Jeong Man Kim1, Kyeong Hee Kim1, Sun Min Lee2, Kwang Won Seo3, Joseph Jeong4, Sung-Ryul Kim4, Seon Ho Lee4, Eun Yup Lee2, Chulhun L. Chang2,5 1Department of Laboratory Medicine, Dong-A University College of Medicine, 2Department of Laboratory Medicine, Pusan National University School of Medicine, Busan; Departments of 3Internal Medicine and 4Laboratory Medicine, Ulsan University College of Medicine, Ulsan; 5Korean Institute of Tuberculosis, The Korean National Tuberculosis Association, Seoul, Korea Corresponding to Chulhun L. Chang, E-mail: cchl@pusan.ac.kr Ann Clin Microbiol 2007;10(2):143-149.Copyright © Korean Society of Clinical Microbiology. Abstract Background: It is recommended that all rapidly growing mycobacteria (RGM) isolated from patients with mycobacteriosis are subjected to antimicrobial susceptibility testing. The current study was aimed to perform susceptibility test on clinical strains of RGM isolated from patients with mycobacteriosis and to determine the clinical significance of the isolates. Methods: For 17 patients with RGM infection from 2002 to 2006 at Ulsan University

Original article PDF Hyukmin Lee1, Chang Ki Kim2, Jongwook Lee3, Sung-Hee Lee4, Ji Young Ahn5, Seong Geun Hong6, Yeon Jun Park7, Seok Hoon Jeong8, Eui-Chong Kim9, Wee Kyo Lee10, Young Uh11, Jong Hee Shin12, Tae Yeal Choi13, Hyo-Sun Kwak14, Kyungwon Lee2 Department of Laboratory Medicine, 1Kwandong University College of Medicine, Goyang; 2Yonsei University College of Medicine, Seoul; 3Keonyang University Medical College, Daejeon; 4Cheju Hanmaeum Hospital, Jeju; 5Sooncheonhyang University College of Medicine, Gumi; 6Pochon CHA University College of Medicine, Seongnam; 7 The Catholic University of Korea College of Medicine, Seoul; 8Kosin University College of Medicine, Busan; 9Seoul National University College of Medicine, Seoul; 10Ajou University College of Medicine, Suwon; 11Yonsei University Wonju College of Medicine, Wonju; 12Chonnam National Univeristy Medical School, Gwangju; 13Hanyang University College of Medicine, Seoul; 14Food Microbiology Division, Center for Food Safety Evaluation, Korea Food and Drug Administration, Seoul, Korea Corresponding to Kyungwon Lee, E-mail: leekcp@yumc.yonsei.ac.kr Ann Clin Microbiol 2007;10(1):59-69.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Emergence and spread of antimicrobial

Original article PDF Chang-Ki Kim1, Jong Hwa Yum2, Dongeun Yong3,4, Seok Hoon Jeong3,4, Kyungwon Lee3,4, Yunsop Chong3,4 1Korean Institute of Tuberculosis, The Korean National Tuberculosis Association, Seoul; 2Department of Biomedical Laboratory Science, Dong-Eui University College of Natural Science, Busan; 3Department of Laboratory Medicine and 4Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea Corresponding to Kyungwon Lee, E-mail: leekcp@yuhs.ac Ann Clin Microbiol 2008;11(2):90-97.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Clinical isolates of AmpC β-lactamase- producing Enterobacteriaceae were evaluated to determine the prevalence of CTX-M extended-spectrum β-lactamases (ESBLs) and their genetic environments. Methods: A total of 250 non-duplicate isolates of Eneterobacter aerogenes, E. cloacae, Citrobacter freundii, Serratia marcescens and Morganella morganii were collected at a Korean hospital. ESBL production was determined by double disk synergy test. For ESBL producers, bla genes were sequenced and blaCTX-Menvironment was characterized by PCR mapping and sequencing. Results: Among the 250 isolates 29 (11.6%) produced ESBL, and 14 of the 29 isolates produced CTX-M ESBLs, including CTX-M-9 by

Original article PDF Sue Jung Kim1, Heejung Kim1, Myung Sook Kim1, Eunmi Koh1, Chang-Ki Kim2, Seok Hoon Jeong1, Yunsop Chong1, Kyungwon Lee1 1Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine; 2Korean Institute of Tuberculosis, Seoul, Korea Corresponding to Kyungwon Lee, E-mail: leekcp@yuhs.ac Ann Clin Microbiol 2008;11(2):112-116.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Toxin immunoassay is widely used for rapid diagnosis of Clostridium difficile-associated diarrhea. The aim of this study was to evaluate the performance of Tox A/B Quik Chek test (TECHLAB, Blacksburg, VA, USA) compared to toxigenic culture. Methods: From September 2006 to August 2007, 959 stools were examined by Tox A/B Quik Chek test and toxigenic culture (C. difficile culture plus tcdB PCR using colonies obtained from culture). Results: Compared to the results of toxigenic culture, the sensitivity and specificity of Tox A/B Quik Chek test were 47.5% and 97.5%, respectively. Conclusion: The sensitivity