Original article PDF Choon-Mee Kim1*, In Sun Choi2*, Sook Jin Jang2, Na-Ra Yun3, Dong-Min Kim3, Donghoon Lim4, Young-Joon Ahn5, Seong Ho Kang2, Geon Park2, Dae Soo Moon2 1Premedical Science, College of Medicine, Chosun University, Departments of 2Laboratory Medicine, 3Internal Medicine, 4Urology, 5Medical Education, College of Medicine, Chosun University, Gwangju, Korea Corresponding to Sook Jin Jang, E-mail: sjbjang@chosun.ac.kr Ann Clin Microbiol 2017;20(4):90-96. https://doi.org/10.5145/ACM.2017.20.4.90Copyright © Korean Society of Clinical Microbiology. Abstract Background: Tigecycline resistance has emerged recently and has shown diverse mechanisms. The aim of this study was to assess the role of efflux activity in tigecycline resistance in 120 clinical isolates of A. baumannii using two methods: the H33342 accumulation assay and adeB real-time reverse transcriptase polymerase chain reaction. In addition, we analyzed the correlation between the expression level of adeB and H33342 accumulation level. Methods: A. baumannii clinical isolates was divided into tigecycline-resistant (49 strains), intermediate (40 strains), and susceptible (31 strains) groups. The H33342 accumulation was measured in the absence or

Original article PDF Si Hyun Kim1*, Eun Hee Park2*, In Yeong Hwang2, Hyukmin Lee3, Sae Am Song4, Miae Lee5, Seungok Lee6, Soo Young Kim6, Jin Ju Kim7, Jong Hee Shin8, Seong Geun Hong9, Kyeong Seob Shin10, Sunjoo Kim11, Nam Hee Ryoo12, Woonhyoung Lee13, Sook Jin Jang14, Jeong Hwan Shin4,15 1Department of Clinical Laboratory Science, Semyung University, Jecheon, 2Department of Infectious Disease, Busan Institute of Health and Environment, Busan, 3Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, 4Department of Laboratory Medicine, Inje University College of Medicine, Busan, 5Department of Laboratory Medicine, Ewha Womans University College of Medicine, Seoul, 6Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Seoul, 7Department of Laboratory Medicine, Inha University College of Medicine, Incheon, 8Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, 9Department of Laboratory Medicine, CHA Bundang Medical Center, CHA University, Seongnam, 10Department of Laboratory Medicine, Chungbuk National University

Original article Jooyoung Cho1,2*, Jung-Hyun Byun1,3,4*, Sang-Guk Lee1, Kyeongjin Oh1, Beomchan Jeon1, Dongeun Yong1,3, Jeong-Ho Kim1,5 1Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, 2Department of Laboratory Medicine, Wonju Severance Christian Hospital, Yonsei University Wonju College of Medicine, Wonju, 3Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 4Department of Laboratory Medicine, Gyeongsang National University Hospital, Gyeongsang National University College of Medicine, Jinju, 5Department of Laboratory Medicine, Yongin Severance Hospital, Yonsei University College of Medicine, Yongin, Korea *These authors contributed equally to this work. Corresponding to Dongeun Yong, E-mail: deyong@yuhs.ac; Jeong-Ho Kim, E-mail: jeongho@yuhs.ac Ann Clin Microbiol 2021;24(3):83-96. https://doi.org/10.5145/ACM.2021.24.3.3Received on 1 April 2021, Revised on 15 July 2021, Accepted on 19 July 2021, Published on 20 September 2021.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Although urine culture is considered a reference standard for the diagnosis of urinary tract infection (UTI), it is