Case report PDF Jeong Won Shin, M.D., Kyoung Ho Roh., M.D., Kyungwon Lee, M.D. and Yunsop Chong, Ph.D. Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea Corresponding to Kyungwon Lee Ann Clin Microbiol 1999;2(2):220-224.Copyright © Korean Society of Clinical Microbiology. Abstract Group B Streptococcus (GBS, S. agalactiae) is known to be the leading cause of neonatal sepsis and meningitis and the infection has been increasingly noted in adults, particularly in those with underlying diseases. Penicillin G is the drug of choice for GBS infection. However, the MIC of penicillin for GBS is greater than that for S. pyogenes. Therefore some GBS infections may be difficult to be treated. However, in Korea, our knowledge on GBS infection is limited. We observed 7 cases of GBS bacteremia during 1993-1996 in a hospital. Of the 7 patients, 3 were less than one month of age with no known

Original article PDF Yoon Hee Kang, M.D., Soo Jin Choi, M.D., Sang Hyun Hwang, M.D., Young Wook Cho, M.D., Duck-Hee Kim, M.T.*, Mi-Na Kim, M.D. and Chik Hyun Pai, M.D. Department of Clinical Pathology, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Korea Corresponding to Mi-Na Kim Ann Clin Microbiol 1999;2(2):158-166.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Escherichia coli and Klebsiella pneumoniae resistant to 3rd generation cephalosporin have been reported with increasing frequency in tertiary-care hospital in Korea. MicroScan Neg Combo Panel Type 21 (Type 21) contains a 1 µg/mL cepfoxodime (POD) in addition to other screen wells containing ceftazidime, cefotaxime, ceftriaxone, and aztreonam, which are designed for detecting extended-spectrum β-lactamase (ESBL)-producing E. coli and Klebsiella species. We evaluated the Type 21 panel for its ability to detect ESBL. Methods: From November to December in 1998, 496 E. coli and 326 K. pneumoniae strains isolated from clinical specimens were tested with Type 21 panel. The isolates

Original article PDF Eui Chong Kim, M.D.¹,², Dong Young Lee, M.D.¹, Hae Shim Choi, M.S.¹, Se Ik Joo, M.S.¹, Jung Hee Lee, M.S.³, Sang Hyun Kim, M.D.² and Sung Hwan Ban, M.D.³ ¹Department of Clinical Pathology, Seoul National University College of Medicine; ²Clinical Research Institute, Seoul National University Hospital, Seoul; ³Department of Pediatrics, Kumi Hospital, Soonchunhyang University College of Medicine, Kumi, Korea Corresponding to Eui Chong Kim, E-mail: euichong@plaza.snu.ac.kr Ann Clin Microbiol 2000;3(2):94-98.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Shiga toxin-producing Escherichia coli (STEC) was found in several serotypes of E. coli including O157 serotype. Sorbitol-MacConkey agar may be useful for the detection of E. coli O157, but is not helpful for the detection of sorbitol-fermenting STEC other than O157. Moreover, some strains of E. coli O157 can ferment sorbitol. In this study, in situ hybridization using DNA probe of shiga toxin was used for the isolation of STEC from the PCR-positive stool and sequence analysis

Original article PDF Jongwook Lee, M.D., Nam Keum Lee*, M.T., Soo Hwan Pai*, M.D., Pum Soo Kim**, M.D., Won Choi, M.D., Don Hang Lee, M.D., Hyung Gil**, M.D., and Young Soo Kim**, M.D. Department of Clinical Pathology, Konyang University Hospital, Taejon, Departments of Clinical Pathology*, Internal Medicine**, Inha University Medical College, Inchon, Korea Corresponding to Jongwook Lee Ann Clin Microbiol 2000;3(2):111-115.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Helicobacter pylori (H. pylori) is closely associated with gastritis, peptic ulcer and gastric carcinoma. We evaluated the reliability and usefulness of ¹³C-urea breath test (¹³C-UBT) for the detection of H. pylori infection and searched for the cut-off value of the test. Method: We investigated 45 patients, who underwent esophagogastroduodenoscopy with multiple biopsy specimens taken for culture, histology and rapid urease test, and ¹³C-UBT. Sensitivity and specificity of UBT were calculated against the combined biopsy-based test results. Result: Of 45 patients, 26 were found to be H.

Original article PDF Tai Yeal Choi*, Myoung-Hee Ahn, Seo Eun Ha, Han Kyu Choi, and Jae-Sook Ryu Departments of Parasitology and Clinical Pathology, Hanyang University College of Medicine, Seoul, Korea Corresponding to Myoung-Hee Ahn, E-mail: mhahn@hanyang.ac.kr Ann Clin Microbiol 2002;5(1):52-58.Copyright © Korean Society of Clinical Microbiology. Abstract Background: ELISA test is commonly used for diagnosis of parasite infection. This experiment was performed for detecting positive sera against Clonorchis sinensis, Paragonimus westermani, cysticercus, sparganum, Aisakis larvae, Toxoplasma gondii and Trichomonas vaginalis in patient’s sera with ELISA and Western blot analysis. Methods: Two hundred sera were collected from clinical laboratory of Hanyang University Hospital(Seoul, Kuri). Antigens of parasites were prepared from rabbit (C. sinensis), dog (P. westermani), hog (cysticercus, from Yonsei University), patient (sparganum), mackerel (Anisakis larvae), mouse (T. gondii), cultivation in Trypticase-Yeast extract-Maltose medium (T. vaginalis). ELISA and Western blot was conducted with several parasite antigens and patient’s sera. Results: Positive antibody titers of P. westermani, Anisakis, C. sinensis were observed 12.7%, 11.0%, and

Original article PDF Byung Ryul Jeon, Rojin Park, Jeong Won Shin, Tae Youn Choi, Hee Bong Shin1, You Kyung Lee1, Young Jin Choi2, Hwi Jun Kim2, and Jee Young Ahn3 Department of Laboratory Medicine, College of Medicine, Soonchunhyang University, Seoul, Bucheon1, Cheonan2and Gumi3, Korea Corresponding to Tae Youn Choi, E-mail: choity@hosp.sch.ac.kr Ann Clin Microbiol 2004;7(2):105-110.Copyright © Korean Society of Clinical Microbiology. Abstract Background : After an infection with HBV, HBsAg is the first virologic marker detectable in the serum. If anti-HBs against ‘a’ determinant of HBsAg appears, HBsAg will disappear and the patients will recover from the HBV infection in most cases. However, we encounter not infrequently concomitant cases of HBsAg and anti-HBs. In this study we evaluated HBV DNA levels in concomitant cases to aid in the interpretation of these serologic results. Methods : This study included 36 cases with positivity for both HBsAg and anti-HBs in an electrochemiluminescent

Original article PDF Hyun-Ouk Song, Myoung-Hee Ahn, Han-Kyu Choi, Jae-Sook Ryu, Duk-Young Min, and Han-Il Ree Department of Parasitology & Institute of Biomedicine, Hanyang University, College of Mediciene, Department of Parasitology & Institute of Tropical Medicine, College of Medicine, Yonsei University, Seoul, Korea Corresponding to Myoung-Hee Ahn, E-mail: mhahn@hanyang.ac.kr Ann Clin Microbiol 2004;7(1):66-71.Copyright © Korean Society of Clinical Microbiology. Abstract Background : We analysed 205 cases of parasitic infection that were confirmed at Dept. of Parasitology, Hanyang University, College of Medicine from January 1986 to December 2003. Methods : Parasitic worms were observed on gross examination or light microscope after treatment with lactophenol. Stool was examined with formalin-ether method for detection of eggs or protozoal cysts and scotch tape method was used for E. vermicularis eggs. In case of S. stercoralis, stool sample was incubated at 26℃ for 48 to 72 h to confirm filariform larvae. Commercial ELISA kit for T. canis and ELISA test

Original article PDF Jeong Man Kim1, Seok Hoon Jeong2, Daeyoung Seo3, Eun Hee Park4, Eun Ju Song5, Jae-Cheol Choi6, Eun Yup Lee6, and Chulhun L. Chang6 Department of Laboratory Medicine1, Dong-A University College of Medicine; Department of Laboratory Medicine2, Kosin University College of Medicine; Department of Laboratory Medicine3, Dong-Eui Medical Center; Microbiology Division4, Busan Metropolitan City Institute of Health and Environment; Department of Microbiology5, Pusan National University College of Natural Sciences; and Department of Laboratory Medicine6, Pusan National University College of Medicine Corresponding to Chulhun L. Chang, E-mail: cchl@pusan.ac.kr Ann Clin Microbiol 2004;7(1):38-42.Copyright © Korean Society of Clinical Microbiology. Abstract Background : It is important to define a source of infection when outbreak of Legionella infections has occurred. The performance of a molecular strain typing method was evaluated for environmental and clinical isolates of Legionella pneumophila.  Methods : Thirteen environmental strains, eleven clinical isolates and one type strain (ATCC 33152) of Legionella pneumophila were used

Original article PDF Jongwook Lee1*, Sun Moon Kim2, Euyi Hyeog Im2, Young Woo Choi2, Yoon Mee Kim3 , Pum Soo Kim4, and Jae Hag Lee5 Departments of Laboratory medicine1, Internal Medicine2, and Pathology3, Keonyang University Hospital, Daejeon; Department of Internal Medicine4, Inha University Hospital, Incheon; Department of Food and Nutrition5, Seoil College, Seoul, Korea Corresponding to Jongwook Lee, E-mail: lee4@kyuh.co.kr Ann Clin Microbiol 2005;8(1):47-50.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The most commonly used regimen for the eradication of Helicobacter pylori is combination of a proton pump inhibitor, clarithromycin, and two other antibiotics, metronidazole and amoxicillin. The increase in resistance to antibiotics seems to result in a decrease in eradication efficacy for H. pylori. We investigated the prevalence of antibiotic resistance in H. pylori isolated in Daejeon area. Methods: A total of 31 clinical isolates of H. pylori were collected from the patients who underwent upper gastrointestinal endoscopy in Keonyang University Hospital during the period from

Original article PDF Il Kwon Bae1, Yu Nae Lee1, Seok Hoon Jeong1, Kyungwon Lee2, Dongeun Yong2, Jongwook Lee3, Seong Geun Hong4, Eui-Chong Kim5, Yeon Jun Park6, Tae Yeal Choi7, Young Uh8, Jong Hee Shin9, Wee Gyo Lee10, Ji young Ahn11, Sung-Hee Lee12, Gun-Jo Woo13, Hyo-Sun Kwak13 Department of Laboratory Medicine1, Kosin University College of Medicine, Busan; Department of Laboratory Medicine2, Yonsei University College of Medicine, Seoul; Department of Laboratory Medicine3, Keonyang University College of Medicine, Daejeon; Department of Laboratory Medicine4, Pochon Cha University College of Medicine, Sungnam; Department of Laboratory Medicine5, Seoul National University College of Medicine; Department of Laboratory Medicine6, The Catholic University College of Medicine; Department of Laboratory Medicine7, Hanyang University College of Medicine, Seoul; Department of Laboratory Medicine8, Yonsei University Wonju College of Medicine9, Wonju; Department of Laboratory , Chonnam National University College of Medicine, Gwangju; Department of Laboratory Medicine10, Ajou University College of Medicine, Suwon; Department