Serotyping and Antimicrobial Susceptibility of Salmonella Isolated in Korea in 2015

시현 김†1   은희 박†2   인영 황2   혁민 이3   새암 송4   미애 이5   승옥 이6   수영 김6   진주 김7   종희 신8   성근 홍9   경섭 신10   선주 김11   남희 류12   운형 이13   운형 이14   정환 신*415   

1 Department of Clinical Laboratory Science, Semyung University, Jecheon
2 Department of Infectious Disease,Busan Institute of Health and Environment, Busan,
3 Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul
4 Department of Laboratory Medicine, Inje University College of Medicine, Busan
5 Department of Laboratory Medicine, Ewha Womans University College of Medicine, Seoul
6 Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Seoul
7 Department of Laboratory Medicine, Inha University College of Medicine, Incheon,
8 Department of Laboratory Medicine,Chonnam National University Medical School, Gwangju
9 Department of Laboratory Medicine, CHA Bundang Medical Center, CHA University, Seongnam,
10 Department of Laboratory Medicine, Chungbuk National University College of Medicine, Cheongju,
11 Department of Laboratory Medicine, Gyeongsang National University College of Medicine, Jinju
12 Department of Laboratory Medicine, Keimyung University College of Medicine, Daegu
13 Department of Laboratory Medicine, Kosin University College of Medicine, Busan
14 Department of Laboratory Medicine, College of Medicine, Chosun University, Gwangju
15 Paik Institute for Clinical Research, Inje University, Busan,
16 Department of Laboratory Medicine, Seoul National University Boramae Medical Center, Seoul

Abstract

Background: Salmonella is an important pathogen that causes gastroenteritis and sepsis in humans. Recently, changes in serotype prevalence and an increase in antimicrobial resistance have been reported. This study investigated the distribution of Salmonella serotypes and determined the antimicrobial susceptibility of various strains. Methods: We collected 113 Salmonella isolates other than Salmonella serotype Typhi from 18 university hospitals in 2015. The serotypes were identified by Salmonella antisera O and H according to the Kauffman White scheme. Antimicrobial susceptibility tests for 12 antibiotics were performed using the disk diffusion method or E-test. Results: We identified 22 serotypes. Serotype group B (44.2%) was the most common, followed by groups C (34.5%) and D (21.2%). Salmonella I 4,[5],12:i:- (23.0%), S. Enteritidis (16.8%), and S. Typhimurium (12.4%) were the most common species. Resistance rates for ampicillin, chloramphenicol, ceftriaxone, and trimethoprim/sulfamethoxazole were 46.9%, 18.5%, 8.8%, and 5.3%, respectively. The intermediate resistance rate to ciprofloxacin was 29.2%. Six isolates were extended-spectrum β-lactamase (ESBL) producers, including 5 blaCTX-M-15 and 1 blaCTX-M-55. Conclusion: There have been changes in the serotype prevalence and antimicrobial resistance of Salmonella in Korea, with a high prevalence of CTX-M 15-positive strains. Continuous monitoring of Salmonella serotypes and antimicrobial resistance is warranted. (Ann Clin Microbiol 2019;22:55-60)

Keywords

Antimicrobial resistance   Salmonella   Serotype         


Figures & Tables

Table 1. Primers used for PCR amplification and sequencing of ESBL genes

Target genePrimer namePrimer sequences (5’-3’)Amplicon size (bp)
blaCTX-M-1CTX-M-1 FGACTATTCATGTTGTTGTTAWTTC973
CTX-M-1 RTAAGGCGATAAACAAAAACGGA
blaCTX-M-2CTX-M-2 FGAGGGATAATACTAATAGAGGA964
CTX-M-2 RCTGAAGTTCAGGAGCACATT
blaCTX-M-9CTX-M-9 FGAATACTGATGTAACACGGATT962
CTX-M-9 RCTGATCCTTCAACTCAGCAA
blaCTX-M-25CTX-M-25 FGGAATTTAGGCTTCACTCAC972
CTX-M-25 RTTAGAATACAAATAGTAAGTGGAG