Ann Clin Microbiol 2024;27(3):205-214. Verification of the Mycoplasma IST3 for urogenital mycoplasma culture in comparison to the Mycoplasma IST2

Table 1. Evaluation of the performance of the Mycoplasma IST3 kit for analysis of speciation and enumeration of genital mycoplasmas compared to the Mycoplasma IST2 kit
CCU by Mycoplasma IST2 CCU by Mycoplasma IST3
Up ≥ 106 Up ≥ 104 Mh ≥ 104 & Up ≥104 Mh < 104 & Up ≥104 Up ≥ 103 Up < 103 Mh ≥ 106 Mh ≥ 104 No growth Subtotal
Up ≥ 104 5 23 1 3 2 34
Mh < 104 & Up ≥104 4 3a 1 8
Mh ≥ 104 & Up ≥104 2a 1 1 4
Up < 104 1 1
Mh < 104 1 1
Subtotal 5 29 3 1 2 3 1 1 3 48

aFor both IST2 and IST3, one sample in each kit resulted in a color change to red in all cupules, and for these samples, the results for that kit were assigned as Mh ≥ 104 and Up ≥ 104.
Abbreviations: CCU, color-changing units; Up, Ureaplasma species; Mh, Mycoplasma hominis.