Ann Clin Microbiol 2025;29(1):5. Diagnostic performance evaluation of the PowerChek Respiratory Virus Panels 1, 2, 3, and 4 for the detection of respiratory viruses, including SARS-CoV-2
| Discordant target | Specimen ID | Comparator assay | PowerChek (Ct) | Comparator (Ct) | Other concordant targets |
|---|---|---|---|---|---|
| ADV | S45 | Allplex | + (31.80) | – | RSV |
| S55 | Allplex | + (32.49) | – | MPV | |
| S78 | Allplex | + (33.13) | – | HCoV-OC43 | |
| S25 | Allplex | – | + (40.48) | INF A | |
| BoV, EV/HRV | S73 | Allplex | –a) | + (34.82, 40.75) | RSV |
| EV/HRV | S59 | Allplex | + (32.08) | – | RSV |
| S74b) | Allplex | + (26.91) | – | HCoV-NL63 | |
| S80 | Allplex | – | + (40.38) | ADV | |
| HCoV-OC43 | S11 | Allplex | – | + (36.97) | INF B |
| S53 | Allplex | – | + (39.95) | None | |
| S63 | Allplex | – | + (27.60) | None | |
| S77 | Allplex | – | + (24.41) | None | |
| INF A | S12 | Allplex | – | + (39.90) | EV/HRV, HPIV-2 |
| S29 | Allplex | – | + (36.85) | ADV, EV/HRV, HCoV-OC43, RSV | |
| S74b) | Allplex | – | + (36.50) | HCoV-NL63 | |
| RSV | S14 | Allplex | – | + (36.89) | EV/HRV |
| SARS-CoV-2 | S90 | Alinity m | Incc) | + (34.09) | None |
A plus sign (+) indicates a positive result (target detected), whereas a minus sign (–) indicates a negative result (target not detected). Ct values are provided for discordant targets only. For S73, in which more than one discordant viral target was identified, both Ct values are listed in the cell, separated by a comma.
a)EV/HRV (Ct, 34.71) was detected in the PowerChek assay despite a negative (–) result.
b)Both assays detected HCoV-NL63; PowerChek also detected EV/HRV, whereas Allplex detected INF A.
c)PowerChek was inconclusive for SARS-CoV-2 (N gene: Ct, 30.92; ORF1ab gene: Ct, 34.15).
Abbreviations: Ct, cycle threshold; ADV, adenovirus; RSV, respiratory syncytial virus; MPV, human metapneumovirus; HCoV-OC43, human coronavirus OC43; INF, influenza virus; BoV, human bocavirus; EV/HRV, enterovirus/human rhinovirus; HPIV, human parainfluenza virus; Inc, inconclusive; ORF, open reading frame; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2.