Abstract
We report a case of cellulitis caused by a novel Cupriavidus species identified using whole-genome sequence analysis. Subcutaneous tissue biopsies from the left lower leg of a 67-year-old man who suffered from cellulitis were cultured. Round, convex, gray and non-hemolytic colonies were recovered after 72-h incubation. 16S rRNA sequence analysis showed 98.6% similarity with Cupriavidus basilensis DSM 11853(T) in the NCBI database and 99.9% similarity with C. basilensis KF708 in the EzBioCloud database. Genomic analysis using the MiSeq platform (Illumina, USA) and the TrueBac ID database (ChunLab, Korea) revealed that the average nucleotide identity (ANI) of this strain with C. basilensis DSM 11853(T) was 87.6%. The patient was treated with oral cefditoren pivoxil for 9 weeks. This study is the first to report cellulitis caused by Cupriavidus species strain J1218. (Ann Clin Microbiol 2019;22:105 -109)
Keywords
Cellulitis Cupriavidus Whole-genome sequencing 16S rRNA
Figures & Tables
Fig. 1. Circular, convex, gray non-hemolytic colonies of Cupriavidus sp. strain J1218 were grown on BAP after 72 h incubation at 35°C.
Figures & Tables
Table 1. The antimicrobial susceptibility test results of
| Antimicrobial agents | MIC (μg/mL) | Interpretation* | |
| MicroScan | Sensititre | ||
| Amikacin | ≤8 | ≤4 | S |
| Aztreonam | 16 | 8 | S or I |
| Cefepime | ≤1 | - | S |
| Cefotaxime | ≤1 | ≤0.5 | S |
| Ceftazidime | ≤1 | 1 | S |
| Chloramphenicol | ≤8 | - | S |
| Ciprofloxacin | ≤0.5 | ≤0.06 | S |
| Gentamicin | ≤2 | ≤0.5 | S |
| Imipenem | ≤1 | ≤0.5 | S |
| Levofloxacin | ≤1 | - | S |
| Meropenem | ≤1 | ≤0.12 | S |
| Minocycline | ≤4 | - | S |
| Piperacillin-tazobactam | ≤8 | ≤1/4 | S |
| Piperacillin | ≤8 | - | S |
| Tetracycline | ≤4 | - | S |
| Tobramycin | ≤2 | ≤2 | S |
| Trimethoprimsulfamethoxazole | ≤2/38 | ≤1/19 | S |
