Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

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Indexed in KCI, KoreaMed, Synapse, DOAJ
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pISSN 2288-0585 eISSN 2288-6850

Home-made Rapid Urease Test for Detection of Helicobacter pylori Infection

Original article

Annals of Clinical Microbiology (Ann Clin Microbiol) 1999 June Volume 2, Issue 1, pages 54-57.

Home-made Rapid Urease Test for Detection of Helicobacter pylori Infection

Jongwook Lee, M.D., Soo Hwan Pai, M.D., Jong Hyun Nahm, M.D., Jong Won Choi M.D., Bum Su Kim*, M.D., Won Choi*, M.D., Don Haeng Lee*, M.D., Hyung Gil Kim*, M.D. and Young Soo Kim*, M.D.

Departments of Clinical Pathology, Internal medicine*, Inha University Medical College, Inchon, Korea

Abstract

Background:The rapid urease test has been widely used for detection of Helicobacter pylori infection because it is easy, simple and rapid result. The CLO test and PyloriTek test observe color changes after the gastric biopsy specimens are inerted into the test kits. Because H. pylori is not evenly distributed in the gastric mucosa, grinding or mincing of gastric specimens prior to culture enhances isolation rate of H. pylori. Therefore, we evaluated the effects of rubbing the gastric biopsy specimens onto the urea soaked filter paper in this home-made rapid urease test.

Methods:Forty-three patients referred for upper gastrointestinal tract endoscopy were evaluated for H. pylori infection. The home-made rapid urease test was prepared by soaking a piece of Whatman No. 2 filter paper in 2% urea agar. We compared the results of the home-made rapid urease test with histologic examination, gram stain, CLO test and culture.

Results:Of forty-three patients, 28 were found to be H. pylori-positive either by gram stain or by culture. The sensitivity and specificity of CLO test, based on the results of gram stain and culture were 85.7% and 92.9%. The sensitivity and specificity of home-made rapid urease test, based on the results of gram stain and culture were 92.9% and 92.9%.

Conclusions:The home-made rapid urease test is faster and shows a high sensitivity and specificity. (Korean J Clin Microbiol 1999;2:54~57)

Keywords

Helicobacter pylori, CLO test, Home-made rapid urease test