Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

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Indexed in KCI, KoreaMed, Synapse, DOAJ
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pISSN 2288-0585 eISSN 2288-6850

Microplate Identification System of Enterobacteriaceae

Original article

Annals of Clinical Microbiology (Ann Clin Microbiol) 1999 December Volume 2, Issue 2, pages 135-143.

Microplate Identification System of Enterobacteriaceae

Young Uh, Jeong Seog Son, Gyu Yel Hwang, In Ho Jang, Kap Jun Yoon and Dong Min Seo*

Departments of Clinical Pathology and Medical Information Development*, Yonsei University Wonju College of Medicine, Wonju, Korea

Abstract

Background: To access the accuracy and clinical usefulness of microplate identification (ID) system for the identification of Enterobacteriaceae, we compared microplate ID system with API 20E (bioMérieux, Marcy I’Etoile, France)

Methods: Ninety-two cultures of Enterobacteriaceae and one isolate of Aeromonas species were simultaneously identified by microplate ID system and the API 20E. Twenty biochemical tests used in microplate ID system were lactose, sucrose, and H₂S in Kigler’s iron agar media; indole, sucrose, raffinose, arabinose, trehalose, adonitol, dulcitol, sorbitol, cellibiose, methy-red, phenylalanine deaminase, ornithine decarboxylase, lysine decarboxylase, arginine dihydrolase, urease, and citrate in microplate; and oxidase test. The identification was obtained by considering percent likelihood (%ID), modal frequency and ID score method.

Results: Among the 92 cultures of Enterobacteriaceae and one isolate of Aeromonas species, agreement rate of identification according to the %ID between microplate ID system and API 20E were 90.3% to the species level and 97.8% to the genus level.

Conclusions: For the identification of clinical Enterobacteriaceae isolates, the microplate ID system compares favorably with API 20E in identification accuracy and have the advantage of cost-saving and easy to use. (Korean J Clin Microbiol 1999;2:135-143)

Keywords

Enterobacteriaceae, Aeromonas, Microplate identification system, API 20E