Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

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Indexed in KCI, KoreaMed, Synapse, DOAJ
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pISSN 2288-0585 eISSN 2288-6850

Screening of Urine Culture Specimens by Gram Stain, Urinalysis and Urine Microscopic Examinations

Original article

Annals of Clinical Microbiology (Ann Clin Microbiol) 2000 June Volume 3, Issue 1, pages 53-56.

Screening of Urine Culture Specimens by Gram Stain, Urinalysis and Urine Microscopic Examinations

Chulhun L. Chang, M.D., Tae Hee Park, M.D., Joseph Jeong, M.D., Hyung Hoi Kim, M.D. and Weon Joo Hwang, M.T.*

Department of Clinical Pathology, College of Medicine, Pusan National University, Department of Clinical Pathology, Pusan National University Hospital*, Pusan, Korea

Abstract

Background: The purpose of this study was to discover ways to screen urine culture specimens through Gram stains, urine stick analyses and microscopic examinations for the laboratory cost saving.

Methods: One hundred and fifty-eight urine specimens for culture were included. Fifty uL of urine were inoculated onto one well each of 10-well slide, dried on the hot plate, and Gram-stained. The results combined with routine urinalyses including urine nitrite and leukocyte esterase, and pyuria, were compared with the routine culture results.

Results: The screening of bacteriuria by Gram stains, urinalyses and microscopic examinations revealed the high sensitivity (91.9%) and negative predictive value (95.5%) with cost saving of 41.8% of inoculating media. Not considering the Gram stains, the screening revealed 83.8% sensitivity and 92.5% negative predictive value, even if the cost saving of inoculating media were as high as 50.1%.

Conclusion: It was demonstrated that it was sensitive and economic and produced rapid preliminary results to screen bacteriuria by the Gram stains combined with urinalyses and microscopic examinations. (Korean J Clin Microbiol 2000;3(1):53-56)

Keywords

Gram stain, Urine, 10-well slide glass, Screening, Bacteriuria