Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

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pISSN 2288-0585 eISSN 2288-6850

Identification Results of Aerobic Gram-positive Bacteria Isolated from Blood Cultures Using BBL Crystal GP ID System

Original article

Annals of Clinical Microbiology (Ann Clin Microbiol) 2001 June Volume 4, Issue 1, pages 22-27.

Identification Results of Aerobic Gram-positive Bacteria Isolated from Blood Cultures Using BBL Crystal GP ID System

Young Uh, Gyu Yel Hwang, In Ho Jang, Hyeun Gyeo Lee, An Suk Jeoung, Soon Deok Park, Jeong Seog Son, and Kap Jun Yoon

Department of Clinical Pathology, Yonsei University Wonju College of Medicine, Wonju, Korea

Abstract

Background: Although most of aerobic gram-positive bacilli have been considered to be contaminants, gram-positive bacilli should be identified to the species level if they are isolated from sterile body sites such as blood, and from adequately collected clinical specimens if they are the predominant organisms. However, identification of gram-positive bacilli are difficult due to the enormous diversity of these organisms and the small number of readily available commercial identification systems in clinical laboratories. Gram-positive bacilli and coccorods isolated from blood cultures were tested with BBL Crystal Gram-Positive (GP) Identification (ID) system in order to evaluate the system’s usefulness of identifying these bacteria.

Methods : Thirty-seven stock strains of aerobic gram-positive bacteria isolated from blood cultures between October 1998 and November 1999 at Wonju Christian Hospital were simultaneously tested by BBL Crystal GP ID system and API system. Three kinds of API system (API Coryne, API 50 CHB, and API 20 Strep) were tested according to gram stain results. Gram-positive bacilli or gram-positive coccorods consecutively isolated from blood cultures from May to November in 2000 were identified by BBL Crystal GP ID system.

Results : Among the 37 stock strains of aerobic gram-positive bacteria, agreement rate of identification between Crystal GP ID system and API system were 88% to the genus level and 63% to the species level in Bacillus species, and 90% to the genus level in Corynebacterium species. The isolation rate of gram-positive bacteria from blood cultures from May to November in 2000 to the genus level were: Bacillus; 41.9%(18/43), Corynebacterium; 37.2%(16/43), and the other grampositive coccorods; 20.9%(9/43).

Conclusions: Crystal GP ID system is a useful identification system which, when combined with basic microbiological tests, should lead to satisfactory identification results for gram-positive bacteria isolated from blood cultures. (Korean J Clin Microbiol 2001;4:22-27)

Keywords

Gram-positive bacilli, Gram-positive coccorod, Identification, Crystal GP ID System