Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

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pISSN 2288-0585 eISSN 2288-6850

Usefulness of a Disk Method for Detection of Hippurate Hydrolysis by Campylobacter jejuni

Original article

Annals of Clinical Microbiology (Ann Clin Microbiol) 2002 June Volume 5, Issue 1, pages 42-46.

Usefulness of a Disk Method for Detection of Hippurate Hydrolysis by Campylobacter jejuni

Young Uh, Soon Duk Park, Hyun Mi Cho, Hyeun Gyeo Lee, An Suk Jeoung, Mi Kyung Lee, and Kap Jun Yoon

Department of Clinical Pathology, Yonsei University Wonju College of Medicine, Wonju, Korea

Abstract

Background: The test for hippurate hydrolysis is critical for differentiation of C. jejuni and other thermophilic Campylobacter species. So, we evaluated the disk method for detection of hippurate hydrolysis by C. jejuni.

Methods: Twenty-eight Campylobacter species isolated from stool culture were simultaneously tested with disk method for detection of hippurate hydrolysis and polymerase chain reaction (PCR) for hippuricase specific gene. Disk method was tested with difference in incubation time (2 hours vs. 4 hours), hippurate concentration (1%, 2%, and 4%), amount of ninhydrin (50 μL vs. 100 μL), and inoculation method (colony vs. suspension of organism adjusted by turbidity), finally, 24 types of disk methods were performed.

Results: By using hippuricase PCR method as the reference for the detection of hippurate hydrolysis, the disk method showed a sensitivity of 91.7% and a specificity of 100% when two kinds of disk methods were simultaneously performed.

Conclusions: The disk method for detection of hippurate hydrolysis is simple to use and require fewer cells than the tube method do, and should be useful as a routine diagnostic test in clinical laboratory for rapid identification of C. jejuni. (Korean J Clin Microbiol 2002;5(1):42-46)

Keywords

Campylobacter jejuni, Hippuricase, PCR, Hippurate hydrolysis, Disk