Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

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pISSN 2288-0585 eISSN 2288-6850

Antibiogram of Escherichia coli and Klebsiella spp. Detected by Vitek ESBL Test

Original article

Annals of Clinical Microbiology (Ann Clin Microbiol) 2003 June Volume 6, Issue 1, pages 47-51.

Antibiogram of Escherichia coli and Klebsiella spp. Detected by Vitek ESBL Test

Eun Hae Cho, Nam Yong Lee

Department of Laboratory Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea

Abstract

Background:Extended-spectrum β-lactamase (ESBL) producing Escherichia coli and Klebsiella spp. isolates are clinically resistant to all the β-lactams except carapenems and cephamycins. This study was to determine the prevalence of ESBL producing E. coli and Klebsiella spp. and the rates and trends of resistance to extended-spectrum β-lactams and other antimicrobial agents in ESBL producing E. coli and Klebsiella spp.. 

Methods:During the periods of 2002, a total 2,551 clinical isolates of E. coli & Klebsiella spp. were collected from patients of the Samsung medical center, Seoul, Korea. Antimicrobial susceptibility test and determination of ESBL production were performed by Vitek GNS-433 card. 

Results:151/1,594 (9.5%) of E. coli isolates, 128/896 (14.3%) of K. pneumoniaeisolates and 11/61 (18.0%) of K. oxytica were ESBL producing strains. Resistance to cefoxitin and cefepime were 2.4% and 13.4% in ESBL producing isolates. Imipenem had excellent activity against E .coli and Klebsiella spp. (100% susceptible). 

Conclusion: In this study, ESBL-producing E. coli and Klebsiella spp were more resistant to β-lactams including cefepime than ESBL non-producing E. coli and Klebsiella spp.. ESBL producing E. coli and Klebsiella spp. showed a high level of co-resistance with aminoglycosides and fluoroquinolones. Imipenem showed the highest level of activity against E. coli and Klebsiella spp.. (Korean J Clin Microbiol 2003;6(1):47-51)

Keywords

Vitel GNS 433 Card, ESBL, Cefepime, Cefoxitin, Imipenem