Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

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pISSN 2288-0585 eISSN 2288-6850

Prevalence of PER-1 Extended-Spectrum β-Lactamase-Producing Clinical Isolates of Acinetobacter baumannii in a University Hospital, Busan, Korea

Original article

Annals of Clinical Microbiology (Ann Clin Microbiol) 2004 June Volume 7, Issue 1, pages 20-26.


https://doi.org/10.5145/ACM.2004.07.1.20

Prevalence of PER-1 Extended-Spectrum β-Lactamase-Producing Clinical Isolates of Acinetobacter baumannii in a University Hospital, Busan, Korea

Jeong Man Kim1, Hyun Kyung Kang2, Seok Hoon Jeong3.4, Il Kwon Bae3, Su Bong Kwon3, Byung Kyu Cho3, Dool Man Kim3, Hyun Joo Kim4, and A Sung Kim1
Department of Laboratory Medicine, Dong-A University College of Medicine1, Department of Periodontology, Pusan National University Hospital2, Department of Laboratory Medicine, Kosin University, College of Medicine3, Department of Quaility Improvement, Kosin University Gospel Hospital4

Abstract

Background : In recent years, Acinetobacter baumannii isolates acquired resistance to cefepime have increased significantly. The aim of this study was to survey the prevalence of PER-1 extendedspectrum β-lactamase (ESBL)-producing A. baumannii isolates in a University Hospital, Busan, Korea.

Methods :Antimicrobial susceptibilities were tested by the disk diffusion method, and double disk synergy test was performed for screening of ESBL-production. MICs were determined by agar dilution method. The isoelectric points of β-lactamases were determined by isoelectric focusing. Transferability of cefepime-resistance were tested by conjugation. blaPER-1 and blaPER-2 alleles were detected by PCR, and the DNA sequences of amplified products were determined by using the dideoxy-chain termination method.

Results :Among 51 clinical isolates of A. baumannii intermediate or resistant to cefepime, 10 isolates (19.6%) showed positive results in double disk synergy test. PCR-based experiments detected blaPER-1 gene in all the 10 isolates. All the isolates contained three β-lactamase bands: pI 5.3, 7.9, and 9.4. MICs of ampicillin, piperacillin, cephalothin, cefoxitin, cefoperazone, ceftazidime, cefotaxime, cefepime, and aztreonam were >256 mg/L, respectively, and them of imipenem were 8-16 mg/L.

Conclusion :The prevalence of PER-1-producing  strains in Busan was less than that of in Seoul. But an outbreak of infection caused by this strain in an intensive care unit shows that spread of PER-1-producing A. baumannii strains can be anticipated in a near future. Prevention of hospital infection by these resistant microorganisms are needed. (Korean J Clin Microbiol 2004;7(1):20-26)

Keywords

Acinetobacter baumannii, PER-1, Extended-Spectrum β-Lactamase