Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

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pISSN 2288-0585 eISSN 2288-6850

Clonality Analysis Using Pulsed-Field Gel Electrophoresis of Erythromycin Resistant Group A Streptococci

Original article

Annals of Clinical Microbiology (Ann Clin Microbiol) 2004 June Volume 7, Issue 1, pages 27-30.


https://doi.org/10.5145/ACM.2004.07.1.27

Clonality Analysis Using Pulsed-Field Gel Electrophoresis of Erythromycin Resistant Group A Streptococci

Nam Yong Lee, Eun Ha Koh1, and Sunjoo Kim1
Department of Laboratory Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul; Department of Laboratory Medicine1, Institute of Health Sciences1, Gyeongsang National University School of Medicine, Jinju, Korea

Abstract

Background :Antibiotic resistance of group A streptococci (GAS) is increasing nationwide. Pulsed-field gel electrophoresis (PFGE) is useful for investigating genetic relationship among outbreaks of bacterial infection. Erythromycin (EM) resistance is mediated by either ermB, ermTR,or mefA gene. The emm gene encodes M protein which is the most important virulence factor of GAS.

Methods :The clonal relationship among 56 EM resistant GAS isolated from the children with acute pharyngitis in Jinju was investigated by analysis of chromosomal DNA restriction pattern with SmaI enzyme. The ermB and mefA genes were amplified and emm genotype was identified with PCR and sequencing. Their relationship with PFGE pattern was investigated.

Results :The emm genotyes were identified as 2, 3, 12, 18, and 75. Mostly emm 12 had ermB gene, while emm 3, 18 and 75 had mefA resistance gene. All strains with mefA gene were not restricted with SmaI. The emm12 strains showed 5 different PFGE patterns.

Conclusions :The emm genotypes were closely related with resistance genes. Analysis of macrorestriction fragment patterns by PFGE showed that EM resistant GAS were polyclonal at least in Jinju. GAS strains with mefAgene were not restricted with SmaI suggesting mefA gene might inhibit chromosomal digestion with SmaI. (Korean J Clin Microbiol 2004;7(1):27-30)

Keywords

Group A streptococci, PFGE, Erythromycin resistance, Clonality