Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

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pISSN 2288-0585 eISSN 2288-6850

Molecular Strain Typing of Legionella Isolates from Water in Cooling Towers of Big Buildings in Busan, Korea

Original article

Annals of Clinical Microbiology (Ann Clin Microbiol) 2004 June Volume 7, Issue 1, pages 38-42.


https://doi.org/10.5145/ACM.2004.07.1.38

Molecular Strain Typing of Legionella Isolates from Water in Cooling Towers of Big Buildings in Busan, Korea

Jeong Man Kim1, Seok Hoon Jeong2, Daeyoung Seo3, Eun Hee Park4, Eun Ju Song5, Jae-Cheol Choi6, Eun Yup Lee6, and Chulhun L. Chang6
Department of Laboratory Medicine1, Dong-A University College of Medicine; Department of Laboratory Medicine2, Kosin University College of Medicine; Department of Laboratory Medicine3, Dong-Eui Medical Center; Microbiology Division4, Busan Metropolitan City Institute of Health and Environment; Department of Microbiology5, Pusan National University College of Natural Sciences; and Department of Laboratory Medicine6, Pusan National University College of Medicine

Abstract

Background : It is important to define a source of infection when outbreak of Legionella infections has occurred. The performance of a molecular strain typing method was evaluated for environmental and clinical isolates of Legionella pneumophila. 

Methods : Thirteen environmental strains, eleven clinical isolates and one type strain (ATCC 33152) of Legionella pneumophila were used for the analysis of pulsed field gel electrophoresis.

Results : All 25 strains were discriminated into 21 types. Two strains isolated from different locations in a same building showed different types. Each two, four, and two strains were shown as the same PFGE patterns.

Conclusions : Even though PFGE typing of Legionella pneumophila is excellent for strain differentiation, the same pattern does not necessarily indicate the same source of isolates. (Korean J Clin Microbiol 2004;7(1):38-42)

Keywords

Legionella, Pulsed field gel electrophoresis