Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

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pISSN 2288-0585 eISSN 2288-6850

Prevalence of CTX-M-type Extended-Spectrum β-Lactamase-Producing Esherichia coli and Klebsiella pneumoniae Isolates in Korea

Original article

Annals of Clinical Microbiology (Ann Clin Microbiol) 2004 June Volume 7, Issue 1, pages 48-54.


https://doi.org/10.5145/ACM.2004.07.1.48

Prevalence of CTX-M-type Extended-Spectrum β-Lactamase-Producing Esherichia coli and Klebsiella pneumoniae Isolates in Korea

Il Kwon Bae1, Gun Jo Woo2, Seok Hoon Jeong1.4, Kwang Ok Park3, Byung Kyu Cho1, Dool Man Kim1, Su Bong Kwon1, Hyun Joo Kim4, and Hyun Kyung Kang5
Department of Laboratory Medicine, Kosin University College of Medicine1, Division of Food Microbiology, Korea Food and Drug Administration2, Department of Quaility Improvement, Pusan National University Hospital3, Department of Quaility Improvement, Kosin University Gospel Hospital4, Department of Periodontology, Pusan National University Hospital5

Abstract

Background : The aim of this study was to survey the nation wide susceptibilities of Esherichia coli and Klebsiella pneumoniae against cefotaxime and to determine the prevalence of CTX-M-type extended-spectrum β-lactamases(ESBLs).

Methods : During the period of April to June, 2002, E. coli and K. pneumoniae isolates were collected from 13 hospitals. Antimicrobial susceptibilities to cefotaxime were tested by the disk diffusion method. ESBL production was determined by double disk synergy test. Cefotaxime-resistance of the ESBL-producers was transferred to azide-resistant E. coli J53 by conjugation. MICs of β-lactam antibiotics were determined by agar dilution method. Searches for blaCTX-M genes were performed by PCR amplication. pIs of β-lactamases were determined by isoelectric focusing.

Results : Ten percents of E. coli and 35 percents of K. pneumoniae isolates among 260 strains of each were intermediate or resistant to cefotaxime. Twenty-three isolates of E. coli and 78 K. pneumoniae isolates showed positive results in the double disk synergy test. One isolate of E. coli and 2 K. pneumoniae isolates harbored blaCTX-M-3 gene, 2 E. coli isolates harbored blaCTX-M-15 gene, and 2 E. coli and 2 K. pneumoniae isolates harbored blaCTX-M-14 gene.

Conclusion : E. coli and K. pneumoniae isolates producing CTX-M-type ESBLs are not uncommon in Korean hospitals. The spread of CTX-M-type ESBL genes could compromise the future usefulness of 3rd generation cephalosporins and aztreonam for the treatment of E. coli and K. pneumoniae infections. (Korean J Clin Microbiol 2004;7(1):48-54)

Keywords

Extended-Spectrum β-Lactamase, CTX-M-3, CTX-M-14, CTX-M-15.