Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

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Prevalence of CTX-M-type Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Isolates in Korea, 2003

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Original article

Annals of Clinical Microbiology (Ann Clin Microbiol) 2004 December Volume 7, Issue 2, pages 111-118.


https://doi.org/10.5145/ACM.2004.07.2.111

Prevalence of CTX-M-type Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Isolates in Korea, 2003

Jung Hyun Lee1, Il Kwon Bae2, Su Bong Kwon2, Seok Hoon Jeong2,Gun Jo Woo3, Jongwook Lee4, Wee Gyo Lee5, Jung Oak Kang6, Ji Young Ahn7, Seong Geun Hong8, Jong Hee Shin9, Young Uh10, Yeon Jun Park11, Eui-Chong Kim12, Kyungwon Lee13, Dongeun Yong13
Departments of Pediatrics1 and Laboratory Medicine2, Kosin University College of Medicine; Division of Food Microbiology, Korea Food and Drug Administration3; Departments of Laboratory Medicine, Keonyang University College of Medicine4, Ajou University College of Medicine5, Hanyang University College of Medicine6, Sooncheonhyang University College of Medicine7, Pochon CHA University College of Medicine8, Chonnam National University Medical school9 , Yonsei University Wonju College of Medicine10, The Catholic University of Korea College of Medicine11, Seoul National University College of Medicine12, Yonsei University College of Medicine13

Abstract

Background : The aims of this study were to survey nationwide susceptibilities of Escherichia coli and Klebsiella pneumoniae isolates against cefotaxime and to determine the prevalences of CTX-Mtype extended-spectrum β-lactamases(ESBLs).

Methods : During the period of February to July, 2003, E. coli and K. pneumoniae isolates were collected from 12 hospitals. Antimicrobial susceptibilities to cefotaxime were tested by the disk diffusion method. ESBL production was determined by the double disk synergy test. Cefotaxime-resistance of the ESBL-producers was transfered to E. coli DH5αand E. coli Top10-F by transformation. MICs of β-lactam antibiotics were determined by the agar dilution method. Searches for blaCTX-M genes was performed by PCR amplication; pIs of β-lactamases were determined by isoelectric focusing.

Results : Among 230 isolates of E. coli and 232 isolates of K. pneumoniae, 27 (11.7%) and 79 (34.1%) were intermediate or resistant to cefotaxime, respectively. Twenty-four (10.4%) isolates of E. coli and 58 (25.0%) K. pneumoniae isolates showed positive results in the double disk synergy test. Three isolates of E. coli and 13 K. pneumoniae isolates harbored blaCTX-M-3 gene, 4 E. coli isolates harbored blaCTX-M-15 gene, and 1 E. coli and 5 K. pneumoniae isolates harbored blaCTX-M-14 gene.

Conclusion : E. coli and K. pneumoniae isolates producing CTX-M-type ESBLs were not uncommon in Korean hospitals. It is thought that periodical surveys are necessary for inspecting the spread of CTX-M-type ESBL genes are necessary. (Korean J Clin Microbiol 2004;7(2):111-118)

Keywords

Extended-Spectrum β-Lactamase, CTX-M-3, CTX-M-14, CTX-M-15.