Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology
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Ji Hae Kang1, Il Kwon Bae2, Su Bong Kwon2, Seok Hoon Jeong2*, Jongwook Lee3, Wee Gyo Lee4, Jung Oak Kang5, Ji Young Ahn6, Seong Geun Hong7, Jong Hee Shin8, Young Uh9, Yeon Jun Park10, Eui-Chong Kim11, Kyungwon Lee12, Dongeun Yong12, and Gun Jo Woo13
Departments of Pediatrics1and Laboratory Medicine2, Kosin University College of Medicine, Busan; Department of Laboratory Medicine3, Keonyang University College of Medicine, Daejeon; Department of Laboratory Medicine4, Ajou University College of Medicine, Suwon; Department of Laboratory Medicine5, Hanyang University College of Medicine, Guri; Department of Laboratory Medicine6, Sooncheonhyang University College of Medicine, Gumi; Department of Laboratory Medicine7, Pochon Cha University College of Medicine, Sungnam; Department of Laboratory Medicine8, Chonnam National University Medical School, Gwangju; Department of Laboratory Medicine9, Yonsei University Wonju College of Medicine, Wonju; Department of Laboratory Medicine10, The Catholic University of Korea College of Medicine, Seoul; Department of Laboratory Medicine11, Seoul National University College of Medicine, Seoul; Department of Laboratory Medicine12, Yonsei University College of Medicine, Seoul; Division of Food Microbiology13, Center for Food Safety Evaluation, Korea Food and Drug Administration, Seoul, Korea
Corresponding to Seok Hoon Jeong, E-mail: kscpjsh@ns.kosinmed.or.kr
Ann Clin Microbiol 2005;8(1):17-25.
Copyright © Korean Society of Clinical Microbiology.
Background: The aim of this study is to determine the nationwide prevalence of Ambler class A extended-spectrum β-lactamase (ESBL)-producing Escherichia coliand Klebsiella pneumoniae and to characterize genotypes of ESBLs.
Methods: During the period of February through July, 2003, E. coli and K. pneumoniae isolates were collected from 12 hospitals in Korea. Antimicrobial susceptibilities were tested by disk diffusion method, and ESBL-production was determined by the double-disk synergy test. MICs of β-lactam antibiotics were tested by agar dilution method. Searches for blaTEM , blaSHV , blaCTX-M , blaPER-1 , blaVEB , blaIBC , blaGES and blaTLA genes were performed by PCR amplification, and the genotypes of ESBLs were determined by direct nucleotide sequence analysis of amplified products.
Results: Resistance rates of E. coli (n=246) and K. pneumoniae (n=239) isolates to ceftazidime were 8.5% and 20.1%, respectively. Most prevalent Ambler class A ESBL genotypes in E. coli isolates were blaCTX-M-15 (n=4) and blaCTX-M-3 (n=3), and each of blaCTX-M-14 , blaSHV-12 , and blaTEM-52 gene was also found in one isolate. Most prevalent ESBL genotypes in K. pneumoniae were blaSHV-12 (n=30) and blaCTX-M-3 (n=13), and blaCTX-M-14 (n=5). blaSHV-2a (n=3), blaSHV-5 (n=2), blaTEM-52 (n=1), blaGES-3 (n=2) genes were also found.
Conclusion: CTX-M-type ESBL-producing E. coli and K. pneumoniae isolates are spreading, and a GES-type ESBL has emerged in Korea. (Korean J Clin Microbiol 2005;8(1):17-25)
Extended-spectrum β-lactamase, CTX-M, GES-3, E. coli, K. pneumoniae