Nosocomial opportunistic infections including fungal infections continue to increase with a longer survival of immunocompromised patients. Disseminated candidiasis is the most common nosocomial fungal infection and the frequency of isolation of non-Candida albicans organisms besides C. albicans is increasing as causative organisms. We detected numerous yeast cells incidentally in a peripheral blood smear of an infant with congenital heart disease who was treated with total parenteral nutrition and catheterization, and had a history of antibiotics use during a long hospitalization period. Pichia anomala was isolated from the blood and pleural effusion. (Korean J Clin Microbiol 2005;8(1):94-98)