Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

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pISSN 2288-0585 eISSN 2288-6850

Association of T Antigens with emm Genotypes of Group A Streptococci

Original article

Annals of Clinical Microbiology (Ann Clin Microbiol) 2006 April Volume 9, Issue 1, pages 18-23


https://doi.org/10.5145/ACM.2006.09.1.23

Association of T Antigens with emm Genotypes of Group A Streptococci

Hyun-Ju Jung1 , Eun-Ha Koh2 , Sunjoo Kim2 , Kook-Young Maeng2, and Sung-Ha Kang3
Department of Laboratory Medicine1, Masan Medical Center, Masan; Department of Laboratory Medicine, and Institute of Health Sciences2, Gyeongsang National University School of Medicine, Jinju; and Department of Laboratory Medicine3, Cheju National University School of Medicine, Cheju, Korea

Abstract

Background: T typing has been used as a screening test for epidemiologic studies of group A streptococci (GAS) infections or carriers, and M typing has been performed for virulence studies. However, M typing is difficult to perform in routine laboratories. Recently, genotyping of the emm gene, which encodes the M protein, has become available. We investigated which T antigen is closely associated with a certain emm genotype.

Methods: GAS were collected from the children in Jinju who were asymptomatic carriers (N=349) or had acute pharyngitis (N=122) during the 3 year-period from 2002 through 2004. T typing was performed by a slide aggulutination, and emm genotyping by PCR and DNA sequencing.

Results: More than 90% of T1, T3, T6, T12, T25, and T5/27/44 antigens were associated with emm1, emm3, emm6, emm12 and 22, emm75, and emm44/61 genotypes, respectively; however, other T antigens, such as T2, T4, T7, T11, and B3264, were not associated with any particular emm genotypes.

Conclusion: Several T antigens are so closely associated with particular emm genotypes that one could predict emm genotypes based on the result of T typing. (Korean J Clin Microbiol 2006;9(1):18-23)

Keywords

Group A streptococci, Streptococcus pyogenes, T antigen, emm genotype, Epidemiology, Pharyngitis