Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

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Emergence of CTX-M-12, PER-1 and OXA-30 β-Lactamase-Producing Klebsiella pneumoniae

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Original article

Annals of Clinical Microbiology (Ann Clin Microbiol) 2006 December Volume 9, Issue 2, pages 102-109.

https://doi.org/10.5145/ACM.2006.09.2.102

Emergence of CTX-M-12, PER-1 and OXA-30 β-Lactamase-Producing Klebsiella pneumoniae

Il Kwon Bae1 , Yu Nae Lee1 , Seok Hoon Jeong1, Kyungwon Lee2, Dongeun Yong2, Jongwook Lee3, Seong Geun Hong4, Eui-Chong Kim5, Yeon Jun Park6, Tae Yeal Choi7, Young Uh8, Jong Hee Shin9, Wee Gyo Lee10 , Ji young Ahn11, Sung-Hee Lee12, Gun-Jo Woo13, and Hyo-Sun Kwak13
Department of Laboratory Medicine1, Kosin University College of Medicine, Busan; Department of Laboratory Medicine2, Yonsei University College of Medicine, Seoul; Department of Laboratory Medicine3, Keonyang University College of Medicine, Daejeon; Department of Laboratory Medicine4, Pochon Cha University College of Medicine, Sungnam; Department of Laboratory Medicine5, Seoul National University College of Medicine; Department of Laboratory Medicine6, The Catholic University College of Medicine; Department of Laboratory Medicine7, Hanyang University College of Medicine, Seoul; Department of Laboratory Medicine8, Yonsei University Wonju College of Medicine9, Wonju; Department of Laboratory , Chonnam National University College of Medicine, Gwangju; Department of Laboratory Medicine10, Ajou University College of Medicine, Suwon; Department of Laboratory Medicine11, Sooncheonhyang University College of Medicine, Gumi; Department of Laboratory Medicine12, Cheju Hanmaeum Hospital, Cheju; and Center for Food Safety Evaluation13, Korea Food and Drug Administration, Seoul, Korea

Abstract

Background: The aim of this study was to determine a nation-wide prevalence of Ambler class A and D extended-spectrum β-lactamase (ESBL) in Klebsiella pneumoniae isolates in Korea.

Methods: During the period of April to May 2005, 189 isolates of K. pneumoniae were collected from 11 Korean hospitals. Antimicrobial susceptibilities to ceftazidime and cefotaxime were tested by the disk diffusion method, and ESBL production was determined by double-disk synergy test. Determinants of ceftazidime or cefotaxime-resistance were transferred to Escherichia coli J53 (azide-resistant) by transconjugation. Genotypes of class A and D ESBL genes were determined by PCR amplification and sequencing.

Results: One hundred-sixty isolates of K. pneumoniae showed positive results in double-disk synergy test. The most prevalent ESBL was SHV-12 (n=148). Also detected were genes encoding ESBLs including TEM-52 (n=1), SHV-2a (n=2), CTX-M-3 (n=15), CTX-M-9 (n=6), CTX-M-12 (n=2), CTX-M-14 (n=9), CTX-M-15 (n=1), PER-1 (n=1), GES-5 (n=3), and OXA-30 (n=2) β-lactamases.

Conclusion: With the emergence of CTX-M-12, PER-1, and OXA-30 β-lactamases, the ESBLs in K. pneumoniae isolates are becoming more diverse in Korea. (Korean J Clin Microbiol 2006;9(2):102-109)

Keywords

Klebsiella pneumoniae, CTX-M-12, PER-1, OXA-30, Extended-Spectrum lactamase