Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

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Indexed in KCI, KoreaMed, Synapse, DOAJ
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pISSN 2288-0585 eISSN 2288-6850

Identification of Candida Species by Multiplex Polymerase Chain Reaction

Original article

Annals of Clinical Microbiology (Ann Clin Microbiol) 2006 December Volume 9, Issue 2, pages 119-124.


https://doi.org/10.5145/ACM.2006.09.2.119

Identification of Candida Species by Multiplex Polymerase Chain Reaction

Mi-Kyung Lee1 , Hye-Ryoun Kim1, and Young-Jo Lee2
Department of Laboratory Medicine1, Chung-Ang University College of Medicine; and Seegene Inc.2, Seoul, Korea

Abstract

Background: Polymerase chain reacation (PCR)-based methods have been described for rapid detection and identification of Candida spp. Multiplex PCR assay was developed using internal transcribed spacers and topoisomerase II gene for the accurate identification of Candida species.

Methods: We designed Dual Specificity Oligo (DSO) primers for multiplex PCR. Multiplex PCR was followed by agarose gel electrophoresis to test 8 type strains (C. albicans, C. parapsilosis, C. glabrata, C. tropicalis, C. krusei, C. guilliermondii, C. lusitaniae, C. dubliniensis) and 96 clinical isolates (C. albicans 51 isolates, C. parapsilosis 10 isolates, C. glabrata 10 isolates, C. tropicalis 9 isolates, C. krusei 6 isolates, C. guilliermondii 5 isolates, C. lusitaniae 5 isolates) of Candida spp.

Results: With multiplex PCR using DSO primers, the eight Candida type strains each could be easily differentiated and all 96 clinical isolates were identified as the same species as were identified by the conventional method.

Conclusion: Multiplex PCR followed by electrophoresis can be useful for the simple and rapid identification of Candida species in routine laboratories. (Korean J Clin Microbiol 2006;9(2):119-124)

Keywords

Candida species identification, Multiplex PCR, Internal transcribed spacers, Topoisomerase II