Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

6

Weeks in Review

2

Weeks to Publication
Indexed in KCI, KoreaMed, Synapse, DOAJ
Open Access, Peer Reviewed
pISSN 2288-0585 eISSN 2288-6850

Frequency of Mutation of Codon 249, Overexpression of p53, and Hepatitis B Virus DNA Positivity in Hepatocellular Carcinoma

Original article

Annals of Clinical Microbiology (Ann Clin Microbiol) 2007 October Volume 10, Issue 2, pages 84-89.


https://doi.org/10.5145/ACM.2007.10.2.84

Frequency of Mutation of Codon 249, Overexpression of p53, and Hepatitis B Virus DNA Positivity in Hepatocellular Carcinoma

Geon Park, M.D.1, Sook-Jin Jang, M.D.1,2, Ho-Jong Jeon, M.D.3, Seong-Hwan Kim, M.D.4, Mi-Ja Lee, M.D.3, Jin-Hee Kim, M.S.1, Sung-Heui Shin, M.D.2, Bidur Prasad Chaulagain, M.S.1,2, Dong-Min Kim, M.D.5, Dae-Soo Moon, M.D.1, Young-Jin Park, M.D.1
Departments of 1Laboratory Medicine, 3Pathology, 4General Surgery, 5Internal Medicine, 2Research Center for Resistant Cells, Chosun University Medical School, Gwangju, Korea

Abstract

Background: In hepatocellular carcinoma (HCC), the frequency of p53 mutation and the association with hepatitis B virus (HBV) infection varies with geographic locations and risk factors. The aim of this study was to determine the frequency of codon 249 mutation of p53, p53 overexpression, and HBV DNA positivity and to observe the relationship between them in Korean HCC.

Methods: We analyzed overexpression of p53 in hepatoma tissue from 17 HCC patients by immunohistochemistry (IHC), specific mutations at the third base position of codon 249 by PCR-restriction fragment length polymorphism (PCR-RFLP) method, and presence of HBV by nested PCR.

Results: Although a point mutation at codon 250 was seen in one (5.8%) of 17 patients, no codon 249 mutations were found in the patient cohort. The p53 protein was overexpressed in 4 (23.5%) of 17 HCCs. PCR for HBV DNA from HCCs showed a positivity rate of 82.4% (14 of 17 specimens).

Conclusion: In HCC of this study, HBV infection was not associated with either 249 mutation or overexpression of p53, and overexpression of p53 protein seemed to be related to other than this mutation. (Korean J Clin Microbiol 2007;10:84-89)

Keywords

Hepatocellular carcinoma, Hepatitis B virus, p53 mutation, p53 overexpression, Immunohistochemistry