Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

6

Weeks in Review

2

Weeks to Publication
Indexed in KCI, KoreaMed, Synapse, DOAJ
Open Access, Peer Reviewed
pISSN 2288-0585 eISSN 2288-6850

Comparison of Two Enzyme-Linked Immunosorbent Assays for Detecting Parasitic Diseases

Original article

Annals of Clinical Microbiology (Ann Clin Microbiol) 2008 April Volume 11, Issue 1, pages 56-62.

https://doi.org/10.5145/ACM.2008.11.1.56

Comparison of Two Enzyme-Linked Immunosorbent Assays for Detecting Parasitic Diseases

Hye Ryoun Kim1, Mi Kyung Lee1, Sung Tae Hong2, Jong Yil Chai2
1Department of Laboratory Medicine, Chung-Ang University College of Medicine; 2Department of Parasitology and Tropical Medicine, Seoul National University, Seoul, Korea

Abstract

Background: Serologic tests for specific antibody nowadays are widely employed for the diagnosis of parasitic diseases. Recently, an increasing numbers of kits have adopted enzyme-linked immunosorbent assay (ELISA) for the detection of parasitic antibodies. In this study, we evaluated two ELISA reagents for the diagnosis of parasitic diseases.

Methods: A total of 553 serum and 156 CSF samples were assayed using an in-house micro-ELISA and Genedia Ab ELISA (Green cross PBM, Korea) for Cysticercus, Paragonimus westermani, Clonorchis sinensis, and Sparganum. We reviewed the medical records of all patients. The results from Genedia Ab ELISA kit were compared with those from the in- house micro-ELISA method.

Results: The overall concordance rate between the two ELISA tests was 95.5%. When compared with the clinical information, the sensitivity, specificity, positive predictive value, and negative predictive value of the in-house micro-ELISA were 100%, 99.0∼99.6%, 82.4∼96.4%, and 100%, and the respective figures for Genedia Ab ELISA kit were 92.9~100%, 88.0∼ 97.3%, 41.7∼50%, and 99.9~100% with kappa agreement of 0.53-0.63. Comparison of two ELISA methods showed a significant difference (P<0.05). Retesting of 85 discordant samples showed that the concordance rate of the in-house ELISA was 97.7% and that of Genedia Ab ELISA was 28.2%.

Conclusion: Genedia Ab ELISA kit showed an intermediate level of kappa agreement compared with the in-house ELISA. Further studies are necessary to improve the concordance rate of the two methods, and a careful interpretation of these results is required for a precise diagnosis. (Korean J Clin Microbiol 2008;11:56-62)

Keywords

Enzyme-Linked Immunosorbent Assay (ELISA), Cysticercus, Paragonimus westermani, Clonorchis sinensis, Sparganum