Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

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Indexed in KCI, KoreaMed, Synapse, DOAJ
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pISSN 2288-0585 eISSN 2288-6850

Evaluation of Rapid Assay (Tox A/B Quik Chek) for the Detection of Clostridium difficile Toxins A and B

Original article

Annals of Clinical Microbiology (Ann Clin Microbiol) 2008 December Volume 11, Issue 2, pages 112-116.

https://doi.org/10.5145/ACM.2008.11.2.112

Evaluation of Rapid Assay (Tox A/B Quik Chek) for the Detection of Clostridium difficile Toxins A and B

Sue Jung Kim1, Heejung Kim1, Myung Sook Kim1, Eunmi Koh1, Chang-Ki Kim2, Seok Hoon Jeong1, Yunsop Chong1, Kyungwon Lee1
1Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine; 2Korean Institute of Tuberculosis, Seoul, Korea

Abstract

Background: Toxin immunoassay is widely used for rapid diagnosis of Clostridium difficile-associated diarrhea. The aim of this study was to evaluate the performance of Tox A/B Quik Chek test (TECHLAB, Blacksburg, VA, USA) compared to toxigenic culture.

Methods: From September 2006 to August 2007, 959 stools were examined by Tox A/B Quik Chek test and toxigenic culture (C. difficile culture plus tcdB PCR using colonies obtained from culture).

Results: Compared to the results of toxigenic culture, the sensitivity and specificity of Tox A/B Quik Chek test were 47.5% and 97.5%, respectively.

Conclusion: The sensitivity of Tox A/B Quik Chek test was not high, but the specificity was high. Although Tox A/B Quik Chek test alone is not sufficient to diagnose Clostridium difficile-associated diarrhea, it may aid rapid diagnosis, early treatment and prevention of nosocomial spread of the infection, if supplemented by C. difficile culture or tissue culture cytotoxin assay. (Korean J Clin Microbiol 2008;11: 112-116)

Keywords

Clostridium difficile, Enzyme immunoassay, Clostridium difficile Toxin A, Clostridium difficile Toxin B