Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

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Indexed in KCI, KoreaMed, Synapse, DOAJ
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pISSN 2288-0585 eISSN 2288-6850

Comparison of Two Sputum Processing Methods for Detecting Mycobacterium tuberculosis by Culture and PCR: Universal Sample Processing (USP) and NALC-NaOH Methods

Original article

Annals of Clinical Microbiology (Ann Clin Microbiol) 2009 June, Volume 12, Issue 2, pages 67-71.

https://doi.org/10.5145/ACM.2009.12.2.67

Comparison of Two Sputum Processing Methods for Detecting Mycobacterium tuberculosis by Culture and PCR: Universal Sample Processing (USP) and NALC-NaOH Methods

Hyeong-Kee Yun1, Soo-Hyun Kim1, Duck Cho1, Seung-Jung Kee1, Myung-Geun Shin1, Jong-Hee Shin1,2, Soon-Pal Suh1, Dong-Wook Ryang1
1Department of Laboratory Medicine, Chonnam National University Medical School and 2Clinical Trial Center, Chonnam National University Hospital, Gwangju, Korea

Abstract

Background: The universal sample processing (USP) method has recently been introduced as a simple technique that is applicable to smear microscopy, culture, and polymerase chain reaction (PCR) for the detection of Mycobaterium tuberculosis (MTB). The present study evaluated the utility of the USP method for detecting MTB by culture and PCR, and the results were compared with that of the N-acetyl L-cysteine (NALC)-NaOH (6%) method.

Methods: All sputum specimens were digested and decontaminated by both the USP and NALC-NaOH methods, and the processed samples were inoculated for MTB culture and PCR. Culture was performed (252 samples) by using the MGIT system (Becton Dickinson Microbiology Systems, Sparks, Md, USA), and PCR test was conducted (281 samples) by using Amplicor MTB kit (Roche Molecular Systems, Branchburg, N.J., USA).

Results: MTB culture positive rates by NALC-NaOH and USP methods were 13.5% (34/252) and 11.9% (30/252), respectively (P>0.05). There were no significant differences between the two methods for detecting MTB by PCR: the MTB PCR sensitivities by USP and NALC-NaOH methods were 77.8% (49/63) and 82.5% (52/63), respectively, and the specificities were 95.9% (209/218) and 96.3% (210/218), respectively (P>0.05).

Conclusion: There were no significant differences between USP and NALC-NaOH methods of sample processing in enhancing the detection of MTB by culture or PCR. (Korean J Clin Microbiol 2009;12: 67-71)

Keywords

Mycobacterium tuberculosis, NALC-NaOH, MGIT, PCR