Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

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pISSN 2288-0585 eISSN 2288-6850

Evaluation of the Vitek 2 AST-N055 Card for the Susceptibility Testing of Acinetobacter baumannii Isolates to Amikacin

Research note

Annals of Clinical Microbiology (Ann Clin Microbiol) 2009 September, Volume 12, Issue 3, pages 144-145

https://doi.org/10.5145/ACM.2009.12.3.144

Evaluation of the Vitek 2 AST-N055 Card for the Susceptibility Testing of Acinetobacter baumannii Isolates to Amikacin

Dong-Jin Park1, Wonkeun Song1, Taek-Kyung Kim2, Jae-Seok Kim1, Han-Sung Kim1, Kyu Man Lee1
Department of Laboratory Medicine, 1Hallym University College of Medicine, 2Kangnam Sacred Heart Hospital, Seoul, Korea

Abstract

We collected 76 clinical isolates of Acinetobacter baumannii (amikacin MIC by Vitek 2 AST-N055 card: ≤2μg/mL, 11 isolates; 4μg/mL, 19 isolates; 8μg/ mL, 17 isolates; 16μg/mL, 27 isolates; and ≥64μg/ mL, 2 isolates) from a university hospital and evaluated the Vitek 2 AST-N055 card vs the broth microdilution as a reference method for testing susceptibility to amikacin. Vitek 2 AST-N055 card yielded very major errors in 15 isolates (19.7%) and minor errors in 26 isolates (34.2%). Of the 15 isolates shown very major errors, 14 had Vitek 2 MICs ranging from 8 to 16μg/mL. The results of our study suggest strongly that it is unreliable to test the amikacin susceptibility by Vitek 2 AST-N055 card of A. baumannii with the Vitek 2 MICs ranging from 8 to 16μg/mL. In those cases, another susceptibility test, such as broth microdilution (BMD), should be performed to confirm the results. (Korean J Clin Microbiol 2009;12: 144-145)

Keywords

Amikacin, Acinetobacter baumannii, Vitek 2