Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

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pISSN 2288-0585 eISSN 2288-6850

A Case of a 63-bp Deletion in the mpt64 Gene of Mycobacterium tuberculosis Strains Which Showed False Negativity in the Immunochromatographic Assay

Case report

Annals of Clinical Microbiology (Ann Clin Microbiol) 2011 March, Volume 14, Issue 1, pages 36-38.

https://doi.org/10.5145/ACM.2011.14.1.36

A Case of a 63-bp Deletion in the mpt64 Gene of Mycobacterium tuberculosis Strains Which Showed False Negativity in the Immunochromatographic Assay

Chang Eun Yoon, Young Joon Hong, Jin Kyung Lee, Yoon Hwan Chang, Seok-Il Hong

Department of Laboratory Medicine, Korea Cancer Center Hospital, Seoul, Korea

Abstract

Mycobacterium tuberculosis complex (MTBC) is discriminated from non-tuberculous mycobacteria (NTM) via an immunochromatographic assay (ICA) which is based on the reactions of monoclonal antibodies against MPT64, one of the predominant proteins excreted by MTBC. Recently, the authors of the present study discovered SD TB-negative Mycobacterium tuberculosis strains. In addition, sequence analysis of the mpt64 genes in these strains was performed and showed a deletion of 63 bp from nucleotides 196 to 258. In cases of MPT64-negative mycobacterium, the authors recommend performing TB PCR for correct diagnosis. (Korean J Clin Microbiol 2011;14:36-38)

Keywords

Mycobacterium tuberculosis, mpt64 gene, Mutation