Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology


Weeks in Review


Weeks to Publication
Indexed in KCI, KoreaMed, Synapse, DOAJ
Open Access, Peer Reviewed
pISSN 2288-0585 eISSN 2288-6850

A Multicentre Study about Pattern and Organisms Isolated in Follow-up Blood Cultures

Original article

Annals of Clinical Microbiology (Ann Clin Microbiol) 2013 March, Volume 16, Issue 1, pages 8-12.

A Multicentre Study about Pattern and Organisms Isolated in Follow-up Blood Cultures

Jeong Hwan Shin1, Eui Chong Kim2, Sunjoo Kim3, Eun-Ha Koh3, Dong-Hyun Lee3, Sun-Hoi Koo4, Ji-Hyun Cho5, Jae-Seok Kim6, Nam Hee Ryoo7
1Department of Laboratory Medicine, Inje University College of Medicine, Busan, 2Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, 3Department of Laboratory Medicine, Gyeongsang National University School of Medicine, Jinju, 4Department of Laboratory Medicine, Chungnam National University College of Medicine, Daejeon, 5Department of Laboratory Medicine, Wonkwang University Medical School, Iksan, 6Department of Laboratory Medicine, Hallym University College of Medicine, Chuncheon, 7Department of Laboratory Medicine, Keimyung University School of Medicine, Daegu, Korea


Background: This study analysed patterns of requests for repeated blood cultures and the microorganisms isolated in follow-up cultures.

Methods: The frequencies and intervals of repeated blood cultures performed during January and February of 2010 at seven university-affiliated hospitals in Korea were evaluated. Results of microbiological cultures at follow-up were analysed with respect to pathogen replication, immune clearance, appearance of new pathogens, and skin contaminants.

Results: Among 3,072 patients who received repeated blood cultures, the average number of requests was 3.2. Of the 5,241 follow-up blood culture events recorded, durations of 1, 2, and 3 days between cultures were identified for 23.1%, 21.4%, and 15.0% of events, respectively. Relative to each initial culture, persistent pathogen growth in subsequent culture(s) accounted for 2.3% of events, whereas immune clearance was confirmed in 8.5% of events. Previously undetected pathogens were isolated in 5.2% of the follow-up cultures, the majority of which grew after an interval of six days. Skin contaminants were detected in 7.6% of the repeated cultures, and 76.1% of the follow-ups displayed no growth of microorganisms.

Conclusion: The most common numbers of repeat culture requests were two and three, and these were typically performed within three days of the initial culture. Among the follow-up cultures, new pathogens were identified in 5.2%, and the majority of this group likely presented for follow-up during a new disease episode. (Ann Clin Microbiol 2013;16:8-12)


Bacteraemia, Follow-up study, Sepsis