Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

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pISSN 2288-0585 eISSN 2288-6850

Molecular Characteristics of blaOXA-23-Producing Acinetobacter baumannii Isolated from a University Hospital

Original article

Annals of Clinical Microbiology (Ann Clin Microbiol) 2013 September, Volume 16, Issue 3, pages 126-133.

https://doi.org/10.5145/ACM.2013.16.3.126

Molecular Characteristics of blaOXA-23-Producing Acinetobacter baumannii Isolated from a University Hospital

In-Ho Jang1, Soon Deok Park1, Young Uh1, Gyu-sang Lee2, Jong-Bae Kim2, Il Choi3
1Department of Laboratory Medicine, Yonsei University Wonju College of Medicine, 2Department of Biomedical Laboratory Science, College of Health Science, Yonsei University, 3Department of Animal Science and Technology, College of Life Science and Natural Resources, Sangi University, Wonju, Korea

Abstract

Background: Multi-drug resistant (MDR) Acinetobacter baumannii has emerged as a significant infectious agent in hospitals worldwide. The purpose of this study was to determine the molecular characterization of MDR A. baumannii clinical isolates.

Methods: Two hundred eighty-five strains of non-duplicated A. baumannii collected from March to November 2011 from a university hospital laboratory located in the Wonju area of the Gangwon province of Korea were analyzed for MDR genes.

Results: All of the 285 imipenem-resistant A. baumannii isolates were encoded by a blaOXA-23-like gene, and all isolates with the blaOXA-23-like gene had the upstream element ISAba1. The 16S rRNA methylase gene armA was detected in 153 (50.2%) clinical isolates, but rmtA, rmtB, rmtC, rmtD and npmA were not detected in any isolates in the present study. The gene encoding aac(6′)-Ib was the most prevalent aminoglycoside-modifying enzyme. The sequencing data for the quinolone resistance-determining region of gyrA and parC revealed the presence of Ser (TCA) 83 to Leu (TTA) and Ser (TCG) 80 to Leu (TTG) substitutions. All but one of the 285 A. baumannii isolates showed similar band patterns on repetitive extragenic palindromic-PCR profiles.

Conclusion: The molecular characteristics of the resistance genes of MDR A. baumannii isolates obtained from the Wonju area of Gangwon province were similar to those of other areas in Korea. (Ann Clin Microbiol 2013;16:126-133)

Keywords

Acinetobacter baumannii; Beta-lactamases; Genes, Multidrug resistance; Imipenem