Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

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Indexed in KCI, KoreaMed, Synapse, DOAJ
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pISSN 2288-0585 eISSN 2288-6850
Original article

Annals of Clinical Microbiology (Ann Clin Microbiol) 2013 December, Volume 16, Issue 4, pages 168-173.

https://doi.org/10.5145/ACM.2013.16.4.168

Effect of Sodium Citrate on Growth of Bacteria in Blood Culture

Dong-Hyun Lee1, Eun-Ha Koh1, Sae-Rom Choi1, Sunjoo Kim1, Dong Hoon Kim2, Nam Yong Lee3
Departments of 1Laboratory Medicine, 2Emergency Medicine, Gyeongsang Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju, 3Department of Laboratory Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea

Abstract

Effect of Sodium Citrate on Growth of Bacteria in Blood Culture

Background: This study compared the growth of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Streptococcus pneumoniae, and Haemophilus influenzae in blood culture bottles containing anticoagulants, sodium polyanethol sulfonate (SPS) and sodium citrate.

Methods: One hundred and fifty colony forming units of five different bacterial species were inoculated into standard aerobic (SA) and standard anaerobic (SN) bottles and were combined with 5 mL of human blood in solution with SPS or sodium citrate. Time to detection (TTD) was then monitored using the BacT/ Alert 3D system (bioMerieux Inc.).

Results: Compared to the bacteria-only controls, cultures containing S. aureus, E. coli, P. aeruginosa, and S. pneumoniae plus SPS blood or citrated blood trended toward reduced TTD in both SA and SN bottles; however, there was no significant difference in TTD between SPS and sodium citrate anticoagulant. Although H. influenzae showed a remarkable difference in TTD between SPS (SA 14.8 h, SN 15.0 h) and sodium citrate (SA 23.5 h, SN 18.3 h), this difference was not statistically significant (P=0.10).

Conclusion: Addition of blood enhanced growth of bacteria. All experimental bacteria except H. influenzae showed similar TTD in SPS blood and citrated blood. These results support the usefulness of sodium citrate anticoagulant for artificial inoculation in blood culture bottles. (Ann Clin Microbiol 2013;16: 168-173)

Keywords

Anticolagulants, Bacteremia, Blood, Culture, Sodium citrate