Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

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pISSN 2288-0585 eISSN 2288-6850

Characterization of Salmonella spp. Clinical Isolates in Gyeongsangbuk-do Province, 2012 to 2013

Original article

Annals of Clinical Microbiology (Ann Clin Microbiol) 2014 June, Volume 17, Issue 2, pages 50-57.

https://doi.org/10.5145/ACM.2014.17.2.50

Characterization of Salmonella spp. Clinical Isolates in Gyeongsangbuk-do Province, 2012 to 2013

Oh-Geun Kweon1, Jin Seok Kim2, Gou-Ok Kim1, Chang-Il Lee1, Kwang-Hyeon Jeong1, Junyoung Kim2
1Division of Microbiology, Gyeongsangbuk-do Provincial Government Institute of Health and Environment, 2Division of Enteric Diseases, Center for Infectious Diseases, Korea National Institute of Health, Cheongwon, Korea

Abstract

Background: Extended-spectrum cephalosporins and fluoroquinolones are important antimicrobials for treating invasive salmonellosis, and emerging resistance to these antimicrobials is of paramount concern.

Methods: A total of 30 Salmonella spp. clinical isolates recovered in Gyeongsangbuk-do from 2012 to 2013 were characterized using antibiotic resistance profiles and pulsed-field gel electrophoresis (PFGE).

Results: A high prevalence of multidrug-resistant isolates, mainly showing an ampicillin, nalidixic acid, chloramphenicol resistance pattern, was observed. Four extended-spectrum β-lactamase (ESBL)-producing isolates (3 CTX-M-15 isolates and 1 CTX-M-27 isolate) were found. The blaCTX-M-27 gene was carried by an IncF conjugative plasmid in the S. Infantis isolate. The blaCTX-M-15 gene were carried by an IncF (2 isolates) or IncHI2 (1 isolate) conjugative plasmid in S. Enteritidis. In addition, a single mutation of GyrA, Ser83Thr (1 isolates), Asp87Tyr (9 isolates), Asp87Gly (4 isolates), and Asp87Leu (3 isolates), was detected in nalidixic acid-resistant Salmonella spp. isolates. XbaI PFGE analysis of all isolates revealed more than 19 different pulsotypes. The most common S. Enteritidis PFGE pattern (SEGX01.003) was associated with a larger number of cases of invasive salmonellosis than all other patterns.

Conclusion: The information from our study can assist in source attribution, outbreak investigations, and tailoring of interventions to maximize disease prevention. (Ann Clin Microbiol 2014;17:50-57)

Keywords

Drug resistance, Extended-spectrum beta-lactamase, Nalidixic acid, Pulsed-Field Gel Electrophoresis, Salmonella