Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

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pISSN 2288-0585 eISSN 2288-6850

A Study of Efflux Pump Genes in Mycobacterium tuberculosis Clinical Isolates

Research note

Annals of Clinical Microbiology (Ann Clin Microbiol) 2014 June, Volume 17, Issue 2, pages 65-68.

https://doi.org/10.5145/ACM.2014.17.2.65

A Study of Efflux Pump Genes in Mycobacterium tuberculosis Clinical Isolates

Sun Young Cho1, You Sun Nam2, Hyung-Seok Yang1, Yun Soo Soh3, Min Jin Kim4, Hee Joo Lee1
1Department of Laboratory Medicine, School of Medicine, Kyung Hee University, 2Department of Biomedical Science, 3Department of Medicine, Kyung Hee University Graduate School, 4Seegene Medical Foundation, Seoul, Korea

Abstract

The efflux pump system has been suggested as an important mechanism in the drug resistance of Mycobacterium tuberculosis (MTB). In this study, molecular analysis of five genes in the efflux pump system of MTB isolates from Korean patients was performed in order to identify appropriate molecular targets. In this study, 35 culture-positive specimens were included. PCR was performed for five efflux genes, mmpL7, efpA, mmr, p55 and tap-like gene. In the 35 clinical isolates, molecular analysis of five kinds of efflux pump genes was performed. Only one clinical isolate showed negative PCR results for all five efflux pump genes. All the rest 34 isolates presented concurrent positive results for the five efflux pump genes. In the near future, gene expression study with quantitative PCR should be performed using these genes. (Ann Clin Microbiol 2014;17:65-68)

Keywords

Multidrug efflux pump genes, Mycobacterium tuberculosis, Drug resistance