Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

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pISSN 2288-0585 eISSN 2288-6850

Identification of Erysipelothrix rhusiopathiae by DNA Sequencing in a Culture-Negative Intra-Abdominal Abscess

Case report

Annals of Clinical Microbiology (Ann Clin Microbiol) 2014 December, Volume 17, Issue 4, pages 132-135.

https://doi.org/10.5145/ACM.2014.17.4.132

Identification of Erysipelothrix rhusiopathiae by DNA Sequencing in a Culture-Negative Intra-Abdominal Abscess

Hee Jae Huh1, Hyun-Young Kim1, Young Eun Ha2, Chang-Seok Ki1, Nam Yong Lee1
1Department of Laboratory Medicine & Genetics, 2Division of Infectious Diseases, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea

Abstract

Erysipelothrix rhusiopathiae is a Gram-positive bacillus that causes infections primarily in animals. In humans, the bacteria usually cause localized or generalized cutaneous infections. A 75-year-old man with chronic alcoholism presented with abdominal pain. Abdominal computed tomography and laboratory findings suggested an intra-abdominal abscess in the periaortic soft tissue. While no definitive infectious source was identified, E. rhusiopathiae was identified by 16S rRNA-based gene sequencing from culture-negative, periaortic necrotic tissue, subsequent to empiric antibiotic treatment. It is suggested that E. rhusiopathiae has the potential to cause intra-abdominal abscesses. This case report highlights the usefulness of DNA sequencing to identify pathogens in patients pretreated with antibiotics. (Ann Clin Microbiol 2014;17:132-135)

Keywords

Abscess, Erysipelothrix rhusiopathiae, Sequencing, 16S rRNA