Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

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An Effective Method of RNA Extraction from Mycobacterium tuberculosis

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Research note

Annals of Clinical Microbiology (Ann Clin Microbiol) 2016 March, Volume 19, Issue 1, pages 20-23.

https://doi.org/10.5145/ACM.2016.19.1.20

An Effective Method of RNA Extraction from Mycobacterium tuberculosis

Tae Sang Oh1, Hee Yoon Kang2, You Sun Nam1, Young Jin Kim2, Eun Kyung You2, Min Young Lee2, Sun Young Cho2, Hee Joo Lee2
1Department of Biomedical Science, Graduate School, Kyung Hee University, 2Department of Laboratory Medicine, Kyung Hee University School of Medicine, Seoul, Korea

Abstract

In the RNA-based study, it is important to extract high-quality RNA. However, RNA extraction from Mycobacterium tuberculosis is problematic due to its thick, waxy cell wall rich in mycolic acid, which renders the cells resistant to lysis. Using TRIzol reagent and several powerful bead-beating steps, a high quantity of RNA was obtained. (Ann Clin Microbiol 2016; 19:20-23)

Keywords

Mycobacterium tuberculosis, RNA extraction, Quantitative reverse transcription polymerase chain reaction