Tae Sang Oh1, Hee Yoon Kang2, You Sun Nam1, Young Jin Kim2, Eun Kyung You2, Min Young Lee2, Sun Young Cho2, Hee Joo Lee2
1Department of Biomedical Science, Graduate School, Kyung Hee University, 2Department of Laboratory Medicine, Kyung Hee University School of Medicine, Seoul, Korea
Corresponding to Hee Joo Lee, E-mail: leehejo@khmc.or.kr
Ann Clin Microbiol 2016;19(1):20-23. https://doi.org/10.5145/ACM.2016.19.1.20
Copyright © Korean Society of Clinical Microbiology.
In the RNA-based study, it is important to extract high-quality RNA. However, RNA extraction from Mycobacterium tuberculosis is problematic due to its thick, waxy cell wall rich in mycolic acid, which renders the cells resistant to lysis. Using TRIzol reagent and several powerful bead-beating steps, a high quantity of RNA was obtained. (Ann Clin Microbiol 2016; 19:20-23)
Mycobacterium tuberculosis, RNA extraction, Quantitative reverse transcription polymerase chain reaction
