Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology


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Indexed in KCI, KoreaMed, Synapse, DOAJ
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pISSN 2288-0585 eISSN 2288-6850

Nationwide Survey of Blood Culture Protocol in Clinical Microbiology Laboratories in Korea

Original article

Annals of Clinical Microbiology (Ann Clin Microbiol) 2016 December, Volume 19, Issue 4, pages 97-104.

Nationwide Survey of Blood Culture Protocol in Clinical Microbiology Laboratories in Korea

Young Ah Kim1, Dokyun Kim2, Dongeun Yong2, Kyungwon Lee2
1Department of Laboratory Medicine, National Health Insurance Service Ilsan Hospital, Goyang, 2Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea


Background: The recovery of bacteria from blood can be affected by many factors. Standardization of blood culture methods is important for reliability. Herein, we aimed to investigate blood culture protocols in Korea.

Methods: We performed a multicenter survey with a questionnaire about blood culture practices, which was sent by email to directors and clinical physicians in charge of clinical microbiology laboratories in May 2014. Total data from 18 participating hospitals were analyzed to be used as current baseline data, which is necessary to optimize blood culture protocols.

Results: Many laboratories included recommended blood volume, which is a major factor for bacteria recovery rate. This varied across participating laboratories. For adults, blood sampling of 10 mL was recommended by 10 laboratories and 20 mL was recommended by 5 laboratories. For children who weighed 14-36 kg and less than 14 kg, blood sampling of 10 mL (n=8) and 5 mL (n=7) was recommended, respectively. For neonates, less than 1 mL was recommended by 12 laboratories.

Conclusion: Substantial variations in blood culture protocols were seen across participating clinical microbiology laboratories. Efforts to standardize this protocol should be undertaken. (Ann Clin Microbiol 2016;19:97-104)


Blood culture, Procedure, Sepsis, Standardization