Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

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Indexed in KCI, KoreaMed, Synapse, DOAJ
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pISSN 2288-0585 eISSN 2288-6850

Evaluation of EuDxTM-PN MLC Detection Kit for Detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella pneumophila in Respiratory Specimens

Original article

Annals of Clinical Microbiology (Ann Clin Microbiol) 2017 December, Volume 20, Issue 4, pages 97-102.

https://doi.org/10.5145/ACM.2017.20.4.97

Evaluation of EuDxTM-PN MLC Detection Kit for Detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella pneumophila in Respiratory Specimens

Mi-Kyung Lee1, Heungsup Sung2, Ah Ra Cho3, Hyun Young Chi4
1Department of Laboratory Medicine, Chung-Ang University College of Medicine, 2Department of Laboratory Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, 3 Seoul Clinical Laboratories (SCL), Yongin, 4Samkwang Medical Laboratories, Seoul, Korea

Abstract

Background: Infection by the intracellular bacteria Mycoplasma pneumoniae, Chamydophila pneumoniae, and Legionella pneumophila are common causes of community-acquired pneumonia (CAP). This study describes the evaluation of a new multiplex real-time PCR test, EuDxTM-PN MLC Detection Kit (EUDIPIA), which allows the simultaneous detection of M. pneumoniae, C. pneumoniae, and L. pneumophila in respiratory samples.

Methods: A total of 353 samples were tested using three PCR kits: multiplex PCR (Seeplex PneumoBacter ACE Detection Kit) and two multiplex real-time PCR (EuDxTM-PN MLC Detection Kit and AnyplexTM II RB5 Detection Kit). The results were considered true positives (expanded standard) for M. pneumoniae, C. pneumoniae, and L. pneumophila if they were positive according to any of the three tests.

Results: The sensitivity and specificity of EuDxTM-PN MLC Detection Kit were 93.3-100% and 100%, respectively. The agreement rate and Cohen’s kappa coefficient (value) between EuDxTM-PN MLC Detection Kit and AnyplexTM II RB5 Detection Kit for M. pneumoniae, C. pneumoniae, and L. pneumophila were 70-100% and 0.82-1, respectively.

Conclusion: These results demonstrate that the EuDxTM-PN MLC Detection Kit is a sensitive, specific, and useful screening tool for the detection of atypical pathogens in respiratory samples and can be helpful in selecting appropriate antimicrobial therapy for patients with respiratory infection. (Ann Clin Microbiol 2017;20:97-102)

Keywords

Chamydophila pneumoniae, Legionella pneumophila, Real-time polymerase chain reaction, Mycoplasma pneumoniae, Respiratory tract infection